PLANT BIOLOGY
ANIMAL BIOLOGY
SUBSCRIPTION
E-SUBSCRIPTION
 
MAP
MAIN PAGE

 

 

 

 

Selionova M.I., Trukhachev V.I., Aybazov A-M.M., N.V. Bogolyubova, Sermyagin A.A., Rykov R.A., Zinovieva N.A., Zagarin Metabolic and antioxidant profile of Nubian and Karachay goats (Capra hircus L.) Sel'skokhozyaistvennaya Biologiya [Agricultural Biology], 2024, Vol. 59, № 6, p. 1145-1159.
(how to cite this article)

doi: 10.15389/agrobiology.2024.6.1145eng

UDC: 636.39:591.1

Acknowledgements:
Supported financially by the Russian Science Foundation under Project No. 21-76-20008

 

METABOLIC AND ANTIOXIDANT PROFILE OF NUBIAN AND KARACHAY GOATS (Capra hircus L.)

M.I. Selionova1 ✉, V.I. Trukhachev1, A-M.M. Aybazov1,
N.V. Bogolyubova2, A.A. Sermyagin2, R.A. Rykov2, N.A. Zinovieva2,
A.Yu. Zagarin1

1Russian State Agrarian University — Timiryazev Moscow Agricultural Academy, 49, ul. Timiryazevskaya, Moscow, 127550 Russia, e-mail m_selin@mail.ru (✉ corresponding author), rector@rgau-msha.ru, velikii-1@yandex.ru, azagarin@rgau-msha.ru;
2Ernst Federal Research Center for Animal Husbandry, 60, pos. Dubrovitsy, Podolsk District, Moscow Province, 142132 Russia, e-mail 652202@mail.ru, alex_sermyagin85@mail.ru, brukw@bk.ru, n_zinovieva@mail.ru

ORCID:
Selionova M.I. orcid.org/0000-0002-9501-8080
Sermyagin A.A. orcid.org/0000-0002-1799-6014
Trukhachev V.I. orcid.org/0000-0002-4650-1893
Rykov R.A. orcid.org/0000-0003-0228-8901
Aybazov A-M.M. orcid.org/0000-0002-3704-3210
Zinovieva N.A. orcid.org/0000-0002-6926-2055
Bogolyubova N.V. orcid.org/0000-0002-0520-7022
Zagarin A.Yu. orcid.org/0000-0002-3782-3903

Final revision received September 11, 2023
Accepted October 23, 2023

When assessing animals’ health status under different physiological conditions and under the influence of various factors, it is important to know the reference values of biochemical indicators and parameters of antioxidant defense. Furthermore, to understand the functioning mechanism of the antioxidant system elements in the body, we should investigate the nature of their correlation. This study has revealed for the first time that the metabolic and antioxidant profile in dual-purpose goat breeds depends on their age and on the number of lactations, as well as on the breeding area in relation to the altitude. With an increase in the number of lactations in animals, indicators related to oxidative stress increased, along with the activation of the antioxidant defense system. Our objective was to evaluate the biochemical and antioxidant status of goats depending on the breed and the number of lactations, as well as to determine the correlation between the biochemical parameters of blood and indicators of the antioxidant system of Karachay and Nubian goats. The study was conducted in 2023 on lactating Nubian (n = 43) and Karachay (n = 93) goats (Capra hircus L.). Karachay goats belong to meat-milk breeds, while Nubian goats belong to milk-meat breeds. Animals of different breeds were divided into four groups according to the number of lactations (group 1, group 2, group 3, and group 4). Blood samples for the study were collected during the summer period. Karachay goats were kept on mountain pastures of the North Caucasus (1000 m above sea level), while Nubian goats were kept in the forest-steppe zone of the Moscow region (180 m above sea level). The former were raised in the pasture-stall system and the latter in the stall-pasture system. The diets of goats, consisting mainly of bulky feeds, did not contain components with pronounced antioxidant properties. Biochemical blood parameters were determined using the ChemWell automatic analyzer (Awareness Technology, USA) with reagents from Analyticon Biotechnologies AG (Germany), Spinreact S.A, (Spain), and AO Diakon (Russia). Lipid peroxidation biomarkers were identified based on the concentration of products reacting with thiobarbituric acid (TBA-AP) with kits from OOO Agat-Med (Russia). Antioxidant defense was determined by ceruloplasmin (CP) activity using Revin’s method. The total amount of water-soluble antioxidants (TAWSA) was assessed by the amperometric method on the Color Yauza-01-AA device with a detector (NPO Khimavtomatika, Russia). The TBA-AP/CP ratio was calculated. Statistical analysis of the results was carried out using the software package Microsoft Office Excel 2003. The influence of factors on the variability of characteristics was assessed using multivariate analysis of variance (MANOVA) with generalized linear models (GLM). We found that breed origin significantly affects the concentration of urea, bilirubin, phosphorus, ceruloplasmin, and, to a lesser extent, the activity of alanine aminotransferase (ALT), the content of calcium, TBA-AP, total protein, globulins, phospholipids, and cholesterol. The blood of Karachay goats had a higher content of total protein, globulins, urea, a higher ALT activity, a higher concentration of total bilirubin, calcium, phosphorus, TBA-AP, and the TBA-AP/CP ratio (4.96 to 85.90 %, p ≤ 0.05, p ≤ 0.001), while that of Nubian goats was higher in ceruloplasmin (80.0 %, p ≤ 0.001), cholesterol, and phospholipids (8.64 and 9.58 %). The study identified the dynamics of biochemical indices and indicators of antioxidant defense for dual-purpose goat breeds depending on the number of lactations. The indicators of protein metabolism and magnesium content in blood serum were minimal during the first lactation and maximal during the fourth lactation. The activity of aspartate aminotransferase (AST), the concentration of TBC-AP, the TBC-AP/CP ratio increased significantly during the second lactation. Then, during the third and the fourth lactations, they decreased to the values of the first lactation. The differences in phosphorus concentration, ceruloplasmin and alkaline phosphatase activity were the opposite: decreasing in the second lactation and increasing in subsequent lactations. TAWSA was higher during the first two lactations and decreased in the third and fourth lactations. The nature of changes in the content of TBA-AP and antioxidant defense indicators (TAWSA and CP) showed the specifics of their interaction to ensure antioxidant homeostasis in goats of different ages. For both breeds, correlation analysis revealed a positive correlation between the concentration of TBA-AP and the amount of albumin (r = 0,34-0,44, p ≤ 0,01), cholesterol (r = 0,30-0,43, p ≤ 0,01), phospholipids (r = 0,30-0,41, p ≤ 0,01), and calcium (r = 0,35-0,64, p ≤ 0,01, p ≤ 0,001) and a negative correlation between the content of ceruloplasmin and TAWSA (r = -0,30…-0,40, p ≤ 0,01). The results of the study are relevant for establishing a database of reference intervals for blood biochemical indicators and indicators of antioxidant defense for different dual-purpose goat breeds of different ages.

Keywords: goats, lactation, lipid peroxidation, antioxidant system, blood biochemical parameters, metabolism, correlation.

 

REFERENCES

  1. Getaneh G., Mebrat A., Wubie A., Kendie H. Review on goat milk composition and its nutritive value. Journal of Nutrition and Health Sciences, 2016, 3(4): 1-10 CrossRef
  2. Erokhin A.I., Karasev E.A., Erokhin S.A. Ovtsy, kozy, sherstyanoe delo, 2020, 4: 22-25 CrossRef (in Russ.).
  3. Sakowski T., Kuczyńska B., Puppel K., Metera E., Słoniewski K., Barszczewski J. Relationships between physiological indicators in blood, and their yield, as well as chemical composition of milk obtained from organic dairy cows. Journal of the Science of Food and Agriculture, 2012, 92(14): 2905-2912 CrossRef
  4. Pogorelova N.A., Arkhipenko Yu.A. Vestnik Omskogo gosudarstvennogo agrarnogo universiteta, 2013, 1(9): 65-67 (in Russ.).
  5. Vysokogorskiy V.E., Voronova T.D., Pogorelova N.A. Fundamental’nye issledovaniya, 2014, 3-1: 81-85 (in Russ.).
  6. Sengaliev E.M., Avdeenko V.S., Molchanov A.V., Kozin A.N. Ovtsy, kozy, sherstyanoe delo, 2017, 4: 44-45 (in Russ.).
  7. Molchanov A.V., Avdeenko V.S., Sengaliev E.M. Ovtsy, kozy, sherstyanoe delo, 2018, 3: 58-60 (in Russ.).
  8. Gür S., Türk G., Demirci E., Yüce A., Sönmez M., Özer Ş., Aksu E. Effect of pregnancy and foetal number on diameter of corpus luteum, maternal progesterone concentration and oxidant/antioxidant balance in ewes. Reproduction in Domestic Animals, 2011, 46(2): 289-295 CrossRef
  9. Al-Gubory K.H., Bolifraud P., Germain G., Nicole A., Ceballos-Bicot I. Antioxidant enzymatic defense systems in sheep corpus luteum throughout pregnancy. Reproduction, 2004, 128(6): 767-774 CrossRef
  10. Polkovnichenko P.A. Vestnik NITs MISI: aktual’nye voprosy sovremennoy nauki, 2019, 20: 48-53 (in Russ.).
  11. Celi P., Gabai G. Oxidant/antioxidant balance in animal nutrition and health: the role of protein oxidation. Frontiers in Veterinary Science, 2015, 2: 48 CrossRef
  12. Halliwell B., Gutteridge J.M.C. Free radicals in biology and medicine. 5th edition. Oxford University Press, 2015 CrossRef 
  13. Wang Y., Chun O.K., Song W.O. Plasma and dietary antioxidant status as cardiovascular disease risk factors: a review of human studies. Nutrients, 2013, 5(8): 2969-3004 CrossRef
  14. Surai P.F., Fisinin V.I. Antioxidant-prooxidant balance in the intestine: applications in chick placement and pig weaning. Journal of Veterinary Science & Medicine, 2015, 3(1): 16 CrossRef
  15. Christensen L.L., Selman C., Blount J.D., Pilkington J.G., Watt K.A., Pemberton J.M., Reid J.M., Nussey D.H. Marker-dependent associations among oxidative stress, growth and survival during early life in a wild mammal. Proc. R. Soc. B, 2016, 283(1840): 20161407 CrossRef
  16. Bakhta A.A., Karpenko L.Yu., Andreeva A.B., Enukashvili A.I. Materialy II Mezhdunarodnogo veterinarnogo kongressa «Vetinstanbul group-2015» [Proc. IIInt. Veterinary Congress «Vetinstanbul group-2015»]. St. Petersburg, 2015: 46 (in Russ.).
  17. Al-Hassan M.J., Mohamed H.E., Al-Samawi K.A., Al-Badawi M.A. The influence of pregnancy and lactation on plasma antioxidant status in Aardi goats. International Journal of Veterinary Science and Research, 2016, 2(1): 032-035 CrossRef
  18. Celi P., Di Trana A., Quaranta A. Metabolic profile and oxidative status in goats during the peripartum period. Australian Journal of Experimental Agriculture, 2008, 48(7): 1004-1008 CrossRef
  19. Radin L., Simpraga M., Vince S., Kostelic A., Milinkovic-Tur S. Metabolic and oxidative status of Saanen goats of different parity during the peripartum period. The Journal of Dairy Research, 2015, 82(4): 426-433 CrossRef
  20. Karapehlivan M., Kaya I., Sağ A., Akin S., Özcan A. Effects of early and late lactation period on plasma oxidant/antioxidant balance of goats. Kafkas Universitesi Veteriner Fakultesi Dergisi, 2013, 19: 529-533 CrossRef
  21. Mohammed M.E.A., Brima E.I., Alasidy A., Qurishi N., Algarni M., Alshehri B.M.A. Physicochemical properties and some mineral concentration of milk samples from different animals and altitudes. Open Chemistry, 2022, 20(1): 494-504 CrossRef
  22. Kumar P., Bharti V.K., Mukesh M. Chemometric analysis of antioxidant and mineral elements in colostrum of native and non-native goat breeds to hypoxic conditions at high altitude. Biological Trace Element Research, 2020, 196: 446-453 CrossRef
  23. Çelik H.T., Aslan F.A., Altay D.U., Kahveci M.E., Konanç K., Noyan T., Ayhan S. Effects of transport and altitude on hormones and oxidative stress parameters in sheep. PLoS ONE, 2021, 16(2): e0244911 CrossRef
  24. Kondrakhin I.P., Arkhipov A.V., Levchenko V.I., Talanov G.A., Frolova L.A., Novikov V.E. Metody veterinarnoy klinicheskoy laboratornoy diagnostiki: spravochnik /Pod redaktsiey I.P. Kondrakhina [Methods of veterinary clinical laboratory diagnostics: a handbook. I.P. Kondrakhin (ed.)]. Moscow, 2004 (in Russ.).
  25. Aybazov M.M., Selionova M.I., Seitov M.S., Bikteev Sh.M. Izvestiya Orenburgskogo gosudarstvennogo agrarnogo universiteta, 2022, 5(97): 300-306 CrossRef (in Russ.).
  26. Djuricic D., Dobranic T., Grizelj J., Gracner D., Harapin I., Stanin D., Folnozic I., Getz I., Cvitkovic D., Samardzija M. Concentrations of total proteins and albumins, and AST, AP, CK and GGT activities in the blood serum boer and Saanen goats during puerperium. Reproduction in Domestic Animals, 2010, 46(4): 674-677 CrossRef
  27. Park Y.S., Suzuki K., Mumby S., Taniguchi N., Gutteridge J.M. Antioxidant binding of caeruloplazmin to myeloperoxidase: myeloperoxidase is inhibited, bur oxidase, peroxidise and immunoreactive properties of caeruloplazmin remain intact. Free Radical Research, 2000, 33: 261-265 CrossRef
  28. Vasconcelos S.M.L., Goulart M.O.F., Moura J.B.F., Manfredini V., Benfato M.B., Kubota L.T. Reactive oxygen and nitrogen species, antioxidants and markers of oxidative damage in human blood: the main analytical methods for their determination. Química Nova, 2007, 30: 1323-1338 CrossRef
  29. Antunović Z., Erceg O., Klir Šalavardić Ž., Mioč B., Đidara M., Novoselec J. Hematological and biochemical parameters in the indigenous Croatian white goat in relation to age. Veterinarski Arhiv, 2022, 92(6): 713-722 CrossRef
  30. Milaeva I.V., Maksimov V.I., Zaytsev S.Yu., Dovzhenko N.A. Uchenye zapiski Kazanskoy gosudarstvennoy akademii veterinarnoy meditsiny im. N.E. Baumana, 2010, 201: 263-266 (in Russ.).
  31. Abdelsattar M.M., Vargas-Bello-Pérez E., Zhang N. Age-related changes in blood biochemical composition of Hu sheep. Italian Journal of Animal Science, 2022, 21(1): 1297-1306 CrossRef
  32. Gurgoze S.Y., Icen H. The influence of age on clinical biochemical parameters in pure-bred Arabian mares. Journal of Equine Veterinary Science, 2010, 30(10): 569-574 CrossRef
  33. Stojević Z., Piršljin J., Milinković-Tur S., Zdelar-Tuk M., Beer Ljubić B. Activities of AST, ALT and GGT in clinically healthy dairy cows during lactation and in the dry period. Veterinarski Arhiv, 2005, 75(1): 67-73.
  34. Milaeva I.V., Voronina O.A., Zaytsev S.Yu. Russian Journal of Agricultural and Socio-Economic Sciences, 2017, 2(62): 275-281 CrossRef
  35. Kurilova A.A., Bakhta A.A., Karpenko L.Y., Plemyashov K., Kozitcyna A.I., Balykina A.B., Enukashvili A.I., Polistovskaia P.A., Ershova O.N., Dushenina O.A. PSX-2 antioxidant system characteristics in Saanen goats depending on lactation period. Journal of Animal Science, 2020, 98(Supplement_4): 460-461 CrossRef
  36. Sokolova M.I., Kuz’mina S.S. Mezhdunarodnyy nauchno-issledovatel’skiy zhurnal, 2020, 8-2(98): 53-56 CrossRef (in Russ.).
  37. Sergina S.N., Ilyukha V.A., Baishnikova I.V., Antonova E.P. Zhurnal evolyutsionnoy biokhimii i fiziologii, 2019, 55(1): 51-58 CrossRef (in Russ.).
  38. Gerasimov F.M, Delenyan N.V., Shaov M.T. Formirovanie sistemy protivokislorodnoy zashchity organizma /Pod redaktsiey E.A. Kovalenko [Formation of the body’s antioxygen defense system. E.A. Kovalenko (ed.)]. Moscow, 1998 (in Russ.).

 

back