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Kalinkova L.V., Kuptsova N.A. Genetic characterization of the Russian population of German classic ponies by STR loci and genes affecting coat colour. Sel'skokhozyaistvennaya Biologiya [Agricultural Biology], 2024, Vol. 59, № 6, p. 1108-1117.
(how to cite this article)

doi: 10.15389/agrobiology.2024.6.1108eng

UDC: 636.16.082:575

Acknowledgements:
The authors are grateful to the animals’ owners who provided biological samples for the study, and to the photographer Ekaterina Druz for the exterior photos of the animals.
Supported financially by the Russian Science Foundation (project No. 24-26-00105, https://rscf.ru/en/project/24-26-00105/)

 

GENETIC CHARACTERIZATION OF THE RUSSIAN POPULATION OF GERMAN CLASSIC PONIES BY STR LOCI AND GENES AFFECTING COAT COLOUR

L.V. Kalinkova ✉, N.A. Kuptsova

All-Russian Research Institute for Horse Breeding, Divovo, Rybnoe District, Ryazan Province, 391105 Russia, e-mail genlab.horses.ru@gmail.com (✉ corresponding author), nadin6626@yandex.ru

ORCID:
Kalinkova L.V. orcid.org/0000-0002-7129-3133
Kuptsova N.A. orcid.org/0000-0001-8556-2309

Final revision received March 27, 2024
Accepted May 22, 2024

The German classic pony is a relatively new breed officially registered in 2000. The German classic pony was created in Germany with the aim of breeding horses that are ideal for children. The ponies are smart, good-tempered and energetic, elegant and handsome, looking like a riding mini-horse. The breed is characterized by presence of attractive silver dapple coat colour of various shades. The silver dapple coat colour in horses is the result of the dominant Silver mutation of the PMEL17 gene that affects the black pigment eumelanin. Typically, the Silver gene changes the black colour of hairs of mane and tail to white, flaxen or grey. German classic ponies have been bred in Russia since 2010. At present, the Russian population of German classic ponies is extremely small. At the beginning of 2023, there were 30 purebred brood mares and 9 stallions. The breeding stock is concentrated on one farm. The unique silver coat colour of the ponies is considered one of the most important traits of selection in the breed. The aim of the study was to evaluate the genetic variability using 17 STR markers and to investigate polymorphism of the 4 genes associated with the coat colours in German classic ponies of the Russian population. A total number of 32 animals were genotyped by using markers of genes MC1R, ASIP, PMEL17, MATP and 17 STR markers: AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HMS1, HMS2, HMS3, HMS6, HMS7, HTG4, HTG6, HTG7, HTG10, LEX3, VHL20. Hair samples were collected from the German classic ponies of the farm Koshelev V.V. in 2019. DNA was isolated from hair follicles using the commercial reagent kit ExtraGene™ DNA Prep 200 (Laboratory Isogen, Russia). Genotyping for STR markers was performed using the reagent kit for multiplex analysis COrDIS Horse (GORDIZ, Russia). Based on the testing results, the following parameters were calculated: allele frequencies, the number of observed alleles in each locus (A), the effective number of alleles (Ae), expected (He) and observed (Ho) heterozygosity, and the inbreeding coefficient (FIS). The results revealed a relatively high level of genetic variability in the most of studied STR loci. The number of alleles identified across all evaluated STR loci was 94. The number of alleles per locus varied from 3 to 8 with a mean value of 5.53±0.35. The level of the observed heterozygosity (Ho) for 16 autosomal loci was 0.7051±0.0434, the FIS coefficient was -0.0686±0.0196. It was found that the frequencies of the dominant A allele of the ASIP gene and the dominant E allele of the MC1R gene were 0.313 and 0.688, respectively. The frequency of the mutant Silver allele of the PMEL17 gene was 0,563. The mutant Cremello allele of the MATP gene was not found in the group of genotyped ponies. Given four genes that control pigmentation, 14 different genotype variants were identified in the tested animals, indicating a high level of genetic variability in the population. The most common genotypes in the studied population were associated with the most unusual and attractive coat colors.

Keywords: horses, German classic pony, DNA markers, polymorphism, STR markers, MC1R, ASIP, PMEL17, MATP.

 

REFERENCES

  1. Kuptsova N.A. Konevodstvo i konnyy sport, 2015, 3: 28-30 (in Russ.).
  2. Krasnikov A.S. Ekster’er loshadi [Horse exterior]. Moscow, 1957 (in Russ.).
  3. Castle W.E., Smith F.H. Silver Dapple, a unique color variety among Shetland ponies. Journal of Heredity, 1953, 44(4): 139-145 CrossRef
  4. Arriens H. Farben und Farbvererbung beim Pferd. Islandpferde. Teil III: Farbaufhellungen und Schimmelungen (E-Book-Version). Tierbuchverlag Irene Hohe, 2013.
  5. Brunberg E., Andersson L., Cothran G., Sandberg K., Mikko S., Lindgren G. A missense mutation in PMEL17 is associated with the Silver coat color in the horse. BMC Genetics,2006, 7: 46 CrossRef
  6. Reissmann M., Bierwolf J., Brockmann G.A. Two SNPs in the SILV gene are associated with silver coat colour in ponies. Animal Genetics, 2007, 38(1): 1-6 CrossRef
  7. Chowdhary B.P. Equine genomics. Wiley-Blackwell, 2013.
  8. Reissmann M. Die Farben der Pferde. Cadmos, 2009.
  9. Sponenberg D.P., Bellone R. Equine color genetics. Willey-Blackwell, 2017.
  10. Reissman M., Brockmann A. Analyse verschiedener Langhaaraufhellungen beim Pferd. Züchtungskunde, 2008, 80(6): 491-500.
  11. Marklund L., Moller M.J., Sandberg K., Andersson L. A missense mutation in the gene for melanocyte-stimulating hormone receptor (MC1R) is associated with the chestnut coat color in horses. Mammalian Genome, 1996, 7: 895-899 CrossRef
  12. Rieder S., Taourit S., Mariat D., Langlois B., Guérin G. Mutations in the agouti (ASIP), the extension (MC1R), and the brown (TYRP1) loci and their association to coat color phenotypes in horses (Equus caballus). Mammalian Genome, 2001, 12: 450-455 CrossRef
  13. Mariat D., Taourit S., Guérin G. A mutation in the MATP gene causes the cream coat colour in the horse. Genetics Selection Evolution, 2003, 35(1): 119-133 CrossRef
  14. Bailey E.F., Brooks S.A. Horse genetics. CABI, 2020.
  15. Cunningham E.P., Dooley J.J., Splan R.K., Bradley D.G. Microsatellite diversity, pedigree relatedness and the contributions of founder lineages to thoroughbred horses. Animal Genetics, 2001, 32(6): 360-364 CrossRef
  16. Leroy G., Callede L., Verrier E., Meriaux J.C., Ricard A., Danchin-Burge C., Rognon X. Genetic diversity of a large set of horse breeds raised in France assessed by microsatellite polymorphism. Genetics Selection Evolution, 2009, 41: 5 CrossRef
  17. Ellegren H., Johansson M., Sandberg K., Andersson L. Cloning of highly polymorphic microsatellites in the horse. Animal Genetics, 1992, 23(2): 133-142 CrossRef
  18. Marklund S., Ellegren H., Eriksson S., Sandberg K., Andersson L. Parentage testing and linkage analysis in the horse using a set of highly polymorphic microsatellites. Animal Genetics, 1994, 25(1): 19-23 CrossRef
  19. Bowling A.T., Eggleston-Stott M.L., Byrns G., Clark R.S., Dileanis S., Wictum E. Validation of microsatellite markers for routine horse parentage testing. Animal Genetics, 1997, 28(4): 247-252 CrossRef
  20. Tozaki T., Kakoi H., Mashima S., Hirota K.I., Hasegawa T., Ishida N., Miura N., Choi-Miura N.H., Tomita M. Population study and validation of paternity testing for Thoroughbred horses by 15 microsatellite loci. Journal of Veterinary Medical Science, 2001, 63(11): 1191-1197 CrossRef
  21. Isakova Zh.T., Toktosunov B.I., Kipen V.N., Kalinkova L.V., Talaibekova E.T., Aldasheva N.M., Abdurasulov A.H. Phylogenetic analysis of Kyrgyz horse using 17 microsatellite markers. Russian Journal of Genetics, 2019, 55(1): 100-104 CrossRef
  22. Rukavina D., Zahirović A., Ćrnkić C., Mačkić-Đurović M., Durmić-Pašić A., Kalamujić Stroil B., Pojskić N. Usefulness of the 17-Plex STR kit for Bosnian mountain horse genotyping. Genetika, 2019, 51(2): 619-627 CrossRef
  23. Van de Goor L.H.P., van Haeringen W.A., Lenstra J.A. Population studies of 17 equine STR for forensic and phylogenetic analysis. Animal Genetics, 2011, 42(6): 627-633 CrossRef
  24. Isakova Zh.T., Isaev M.A., Kipen V.N., Kalinkova L.V., Aitbaev K.A., Arzybaev M.A., Mukeeva S.B., Meerim O.K., Aldasheva N.M. Genetic diversity of the Kyrgyz horse breed using microsatellite markers—extended genogeographic study. Russian Journal of Genetics, 2021, 57(4): 438-445 CrossRef
  25. Seo J.-H., Park K.-D., Lee H.-K., Kong H.-S. Genetic diversity of Halla horses using microsatellite markers. Journal of Animal Science and Technology, 2016, 58: 40 CrossRef
  26. Vdovina N.V., Yuryeva I.B. Monitoring for the genetic structure of Mezen breed of horses in terms of DNA microsatellites. Vavilov Journal of Genetics and Breeding, 2021, 25(2): 202-207 CrossRef
  27. Ye J., Coulouris G., Zaretskaya I., Cutcutache I., Rozen S., Madden T. Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction. BMC Bioinformatics, 2012, 13: 134 CrossRef
  28. Brunberg E., Gille S., Mikko S., Lindgren G., Keeling L.J. Icelandic horses with the Silver coat colour show altered behavior in a fear reaction test. Applied Animal Behaviour Science, 2013, 146(1-4): 72-78 CrossRef

 

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