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Chaickaya K.D., Lemyakin A.D., Tyazhchenko A.N., Chaitsky A.A., Schiogolev P.O., Nikiforova A.I., Kofiadi I.A. Association of the leptin gene allelic variants with dairy productivity parameters in cows of Kostroma, Yaroslavl and black-and-white breeds of the Kostroma region. Sel'skokhozyaistvennaya Biologiya [Agricultural Biology], 2024, Vol. 59, № 6, p. 1091-1107.
(how to cite this article)

doi: 10.15389/agrobiology.2024.6.1091eng

UDC: 636.082.12:575.174.015.3

Acknowledgements:
Supported by the Ministry of Agriculture of the Russian Federation Reg. No. R&D 121121300347-5

 

ASSOCIATION OF THE LEPTIN GENE ALLELIC VARIANTS WITH DAIRY PRODUCTIVITY PARAMETERS IN COWS OF KOSTROMA, YAROSLAVL AND BLACK-AND-WHITE BREEDS OF THE KOSTROMA REGION

K.D. Chaickaya1 ✉, A.D. Lemyakin1, A.N. Tyazhchenko1,
A.A. Chaitsky1, P.O. Schiogolev1, A.I. Nikiforova2, I.A. Kofiadi3

1Kostroma State Agricultural Academy, 34, Uchebny gorodok, Karavaevo village, Kostroma District, Kostroma Province, 156530 Russia, e-mail kseniyasabetova@mail.ru (✉ corresponding author), whichspecial@gmail.com, tyazhchenko2024@mail.ru, leha.chaitskiy@mail.ru, bigboy25@mail.ru;
2NPO DNA-Technology LLC, 3, ul. Zheleznodorozhnaya, Protvino, Moscow Province, 142281 Russia, e-mail nikiforova@dna-technology.ru;
3State Research Center Institute of Immunology of the Federal Medical and Biological Agency of Russia, 24, Kashirskoe shosse, Moscow, 115522 Russia, e-mail kofiadi@mail.ru

ORCID:
Chaickaya K.D. orcid.org/0000-0003-3282-4779
Schiogolev P.O. orcid.org/0000-0003-3552-8457
Lemyakin A.D. orcid.org/0000-0002-7737-6351
Nikiforova A.I. orcid.org/0009-0008-3689-1905
Tyazhchenko A.N. orcid.org/0000-0002-6414-7269
Kofiadi I.A. orcid.org/0000-0001-9280-8282
Chaitsky A.A. orcid.org/0000-0002-5853-3809

Final revision received September 08, 2023
Accepted October 15, 2023  

 

The leptin gene is one of the most valuable genetic markers that determine milk productivity, technological features of milk production and productive longevity of cattle, which is of practical importance in breeding work. Conducting marker-associated breeding based on specific signs of productivity and functional longevity of animals using related leptin genotypes contributes to the preservation and multiplication of valuable breeding resources of local cattle breeds, as well as the creation of reference cattle herds. In this work, for the first time, data were obtained on the reliable association of the leptin gene LEP-A80V locus of with economically useful traits in Russian black-and-white cows in the Kostroma region, which proves the importance of these genotypes for characterizing the breeding and productive qualities of livestock in this area. The aim of the work was to study the associative relationships of various allelic variants of the leptin gene with signs of dairy productivity of cows from breeding farms in the Kostroma region. Kostroma, Russian black-and-white and Yaroslavl cows (Bos taurus) from breeding farms of the Kostroma region (2019-2021), a total of 141 cows, including 33, 47 and 61 animals of the corresponding breeds were tested. The data of breeding and zootechnical records were analyzed. Milk productivity was evaluated based on monthly control milking. Whole blood for DNA isolation was taken from the caudal vein into vacuum tubes with the anticoagulant EDTA. DNA was isolated using the DNeasy blood and tissue analysis kit (QIAGEN N.V, Germany). Polymorphic variants of the leptin LEP gene were determined by real-time polymerase chain reaction (RT-PCR) at three loci, the LEP-R25C (alleles R, C; genotypes RR, RC, CC), LEP-A80V (alleles A, V; genotypes AA, AV, VV), and LEP-Y7F (alleles Y, F, genotypes YY, YF, FF) using a DTprime amplifier (DNA Technologies LLC, Russia) and HRM (high resolution melting) technology. It was found that the allele balance at the LEP-A80V locus in Russian black-and-white cows significantly shifts towards homozygous animals, mainly of the AA genotype (0.541±0.064). Homozygous carriers of the A allele have increased milk yield of 8856 kg (a confidence interval of 8819.2-9394 kg), while heterozygous animals had the highest milk fat content of 4.05 % (a confidence interval of 3.99-4.21 %). The allele balance at the LEP-Y7F locus in all the studied groups turned out to be significantly biased to YY homozygotes (0.936-0.970), however, effect of this polymorphism on dairy traits was not revealed. Nevertheless, for Y7F polymorphism, a tendency was revealed towards increased milk yield and fat content in the Russian black-and-white first-calf cows of the YY genotype, by 2275 kg and 0.075 %, respectively. For the LEP-R25C locus, a significant shift in the allele balance occurs only in Russian black-and-white cows towards homozygosity, but no statistically significant effect of this polymorphism on milk productivity was found, probably due to an insufficient number of genotyped animals, which indicates necessity of further research at a larger population.

Keywords: LEP, polymorphic variants, dairy productivity, cattle, Kostroma breed, Yaroslavl breed, Russian black-and-white cows.

 

REFERENCES

  1. Yudin N.S., Luk’yanov K.I., Voevoda M.I., Kolchanov N.A.Vavilovskiy zhurnal genetiki i selektsii, 2015, 3(19): 277-285 CrossRef (in Russ.).
  2. Osipova A.A. Okruzhayushchaya sreda i energovedenie, 2019, 2: 55-82 CrossRef (in Russ.).
  3. Liefers S.C., Veerkamp R.F., te Pas M.F., Delavaud C., Chilliard Y., van der Lende T. Leptin concentrations in relation to energy balance, milk yield, intake, live weight, and estrus in dairy cows. Journal of Dairy Science, 2003, 86(3): 799-807 CrossRef
  4. Komisarek J. Impact of LEP and LEPR gene polymorphisms on functional traits in Polish Holstein-Friesian cattle. Animal Science Papers and Reports, 2010, 28(2): 133-142.
  5. Trakovická A., Moravčíková N., Kasarda R. Genetic polymorphisms of leptin and leptin receptor genes in relation with production and reproduction traits in cattle. Acta Biochim. Pol., 2013, 60(4): 783-787.
  6. Domínguez-Mancera B., Barrientos-Morales M., Cervantes-Acosta P., Hernández-Beltrán A., Rodríguez-Andrade A., González-Ramírez R., Monjaraz E., Felix R. Leptin regulation of inward membrane currents, electrical activity and LH release in isolated bovine gonadotropes. Biochemical and Biophysical Research Communications, 2017, 491(1): 53-58 CrossRef
  7. Kumar V. Genetic and breeding aspects of lactation. In: Trends and advances in veterinary genetics /M. Abubakar (ed.). IntechOpen, London, 2017 CrossRef
  8. Chizhova L.N., Kononova L.V., Sharko G.N., Kovaleva G.P. Sbornik nauchnykh trudov Vserossiyskogo nauchno-issledovatel’skogo instituta ovtsevodstva i kozovodstva, 2017, 2(10): 113-117 (in Russ.).
  9. Sycheva O.V., Kononova L.V. Agrarno-pishchevye innovatsii, 2018, 1(1): 27-31 (in Russ.).
  10. Nasr M.A.F., Awad A., Araby I.E. Associations of leptin and pituitary-specific transcription factor genes polymorphisms with reproduction and production traits in dairy buffalo. Reprod. Dom. Anim., 2016, 51(4): 597-603 CrossRef
  11. Putra W.P.B., Indriastuti R. Leptin gene as potential gene for molecular selection on cattle in Indonesia. Wartazoa, 2017, 27(3): 105-115.
  12. Ma Y., Khan M.Z., Xiao J., Alugongo G.M., Chen X., Chen T., Liu S., He Z.. Wang J., Shah M.K., Cao Z. Genetic markers associated with milk production traits in dairy cattle. Agriculture, 2021, 11(10): 1018 CrossRef
  13. Shchegolev P.O., Sabetova K.D., Chaitskiy A.A., Sorokina A. Svyaz’ polimorfizma gena leptina (LEP) s khozyaystvenno poleznymi priznakami krupnogo rogatogo skota. Agrarnyy vestnik Nechernozem’ya, 2021, 1(1): 25-32 CrossRef
  14. Kulig H. Associations between leptin gene polymorphism and some milk performance traits of cattle. Journal of Animal and Feed Sciences, 2005, 14(2): 235-243 CrossRef
  15. Yoon D.H., Cho B.H., Park B.L., Choi Y.H., Cheong H.S., Lee H.K., Chung E.R., Cheong I.C., Shin H.D.Highly polymorphic bovine leptin gene. Asian-Australasian Journal of Animal Science, 2005, 11(18): 1548-1551 CrossRef
  16. Perchun A.V., Lazebnaya I.V., Belokurov S.G., Egorov O.S., Kuz’menkov I.I., Sulimova G.E. Aktual’nye problemy nauki v agropromyshlennom komplekse: sbornik statey 66-y mezhdunarodnoy nauchno-prakticheskoy konferentsii: v 3 tomakh. Tom. 1 [Current problems of science in the agro-industrial complex: collection of articles of the 66th international scientific and practical conference: in 3 volumes. Volume 1]. Karavaevo, 2015: 199-202 (in Russ.).
  17. Kovalyuk N.V., Satsuk V.F., Machul’skaya E.V., Morkovkina N.A., Shakhnazarova Yu.Yu. Molochnoe i myasnoe skotovodstvo, 2017, 3: 14-16 (in Russ.).
  18. Giblin L., Butler S.T., Kearney B.M., Sinead W.M., Michael C.J., Donagh B.P.Association of bovine leptin polymorphisms with energy output and energy storage traits in progeny tested Holstein-Friesian dairy cattle sires. BMC Genet., 2010, 11: 73 CrossRef
  19. Shevtsova A.A., Klimov E.A., Koval’chuk S.N. Mezhdunarodnyy zhurnal prikladnykh i fundamental’nykh issledovaniy, 2018, 11(1): 194-200 CrossRef (in Russ.).
  20. Bhat S.A., Ahmad S.M., Ganai N.A., Khan Sh.M., Malik A., Shah R.A., Raashid A., Iqbal Z. Association of DGAT1, beta-casein and leptin gene polymorphism with milk quality and yield traits in Jersey and its cross with local Kashmiri cattle. J. Entomol. Zool. Stud.,2017, 5(6): 557-561.
  21. Dar M.R., Singh M., Thakur S., Verma A. Exploring the relationship between polymorphisms of leptin and IGF-1 genes with milk yield in indicine and taurine crossbred cows. Trop. Anim. Health Prod., 2021, 53: 413 CrossRef
  22. Chizhova L.N., Surzhikova E.S., Chudnovets A.I., Sharko G.N., Mikhaylenko T.N. Vestnik APK Stavropol’ya, 2020, 1(37): 34-38 (in Russ.).
  23. Kulig H., Kmiec M., Kowalewska Luczak İ., Andzıak G. Effect of leptin gene polymorphisms on milk production traits of Jersey cows. Turk. J. Vet. Anim. Sci., 2009, 33(2): 143-146 CrossRef
  24. Clempson A.M., Pollott G.E., Brickell J.S., Bourne N.E., Munce N., Wathes D.C. Evidence that leptin genotype is associated with fertility, growth, and milk production in Holstein cows. Journal of Dairy Science, 2011, 94(7): 3618-3628 CrossRef
  25. De Matteis G., Scatà M., Grandoni F., Petrera F., Abeni F., Catillo G., Napolitano F., Moioli B. Association analyses of single nucleotide polymorphisms in the leptin and leptin receptor genes on milk and morphological traits in Holstein cows. Open Journal of Animal Sciences, 2012, 2(3): 174-182 CrossRef
  26. Cañizares-Martínez M.A., Parra-Bracamonte G.M., Segura-Correa J.C. Effect of leptin, pituitary transcription factor and luteinizing hormone receptor genes polymorphisms on reproductive traits and milk yield in Holstein cattle. Brazilian Archives of Biology and Technology, 2021, 64 CrossRef
  27. Zinnatova F.F., Shamsova A.R., Zinnatov F.F., Safiullina A.R., Khamitova L.L. Materialy XII Mezhdunarodnoy nauchno-prakticheskoy konferentsii «Fundamental’naya nauka i tekhnologii — perspektivnye razrabotki» [Proc. XIIInt. Conf. «Fundamental science and technology — promising developments»]. North Charleston, USA,2017: 1-3 (in Russ.).
  28. Kovalyuk N.V., Gyrnets E.A. Universum: khimiya i biologiya: elektron. nauchn. zhurn., 2016, 6(24): 56-60 (in Russ.).
  29. Tyul’kin S.V., Shaydullin R.R., Gil’manov Kh.Kh., Vafin R.R., Faizov T.Kh. Veterinarnyy vrach, 2019, 3: 52-56 CrossRef (in Russ.).
  30. Gaynutdinova E.R. Uchenye zapiski Kazanskoy gosudarstvennoy akademii veterinarnoy meditsiny im. N.E. Baumana, 2021, 1(245): 24-28 CrossRef (in Russ.).
  31. Ahani Azari M., Hasani S., Heidari M., Yousefi S. Genetic polymorphism of leptin gene using PCR-RFLP method in three different populations. Slovak J. Anim. Sci., 2012, 45(2): 39-42.
  32. Safina N.Yu. Vestnik Kazanskogo gosudarstvennogo agrarnogo universiteta, 2017, 4(46): 32-36 CrossRef (in Russ.).
  33. Chebel R.C., Susca F., Santos J.E. Leptin genotype is associated with lactation performance and health of Holstein cows. Journal of Dairy Science, 2008, 91(7): 2893-2900 CrossRef
  34. Zinnatov F.F., Yakupov T.R., Zinnatova F.F., Akhmetov T.M., Ovsyannikov A.P., Chevtaeva N.D. Uchenye zapiski Kazanskoy gosudarstvennoy akademii veterinarnoy meditsiny im. N.E. Baumana, 2022, 250(2): 85-92 CrossRef (in Russ.).
  35. Yaryshkin A.A., Shatalina O.S., Leshonok O.I., Kovalyuk N.V. Izvestiya Orenburgskogo gosudarstvennogo agrarnogo universiteta, 2022, 1(93): 260-264 CrossRef (in Russ.).
  36. Kawaguchi F., Okura K., Oyama K., Mannen H., Sasazaki S. Identification of leptin gene polymorphisms associated with carcass traits and fatty acid composition in Japanese Black cattle. Anim. Sci. J., 2017, 88(3): 433-438 CrossRef
  37. Carvalho T.D., Siqueira F., Almeida Torres Jr. R.A., Medeiros S.R., Feijó G.L.D., Souza Jr. M.D., Blecha I.M.Z., Soares C.O. Association of polymorphisms in the leptin and thyroglobulin genes with meat quality and carcass traits in beef cattle. Revista Brasileira de Zootecnia, 2012, 10(41): 2162-2168 CrossRef
  38. Mandefro A., Sisay T., Kim K.S., Edea Z., Konwarh R., Dadi H. Single nucleotide polymorphisms of leptin gene in five Ethiopian indigenous cattle breeds and the Korean Hanwoo breed. Trop. Anim. Health Prod., 2021, 53(2): 202 CrossRef
  39. Metin Kiyici J., Akyüz B., Kaliber M., Arslan K., Aksel E.G., Çinar M.U. LEP and SCD polymorphisms are associated with milk somatic cell count, electrical conductivity and pH values in Holstein cows. Animal Biotechnology, 2020, 31(6): 498-503 CrossRef
  40. Glantz M., Lindmark Mansson H., Stålhamma H., Paulsson M. Effect of polymorphisms in the leptin, leptin receptor and acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) genes and genetic polymorphism of milk proteins on bovine milk composition. Journal of Dairy Research, 2012, 79(1): 110-118 CrossRef

 

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