Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2017, Vol. 52, № 6, p. 1273-1278
(how to cite this article)

doi: 10.15389/agrobiology.2017.6.1273eng

UDC 632.2:619:579.62:57.083.3

Acknowledgements:
Supported financially by Russian Science Foundation (project № 15-16-00020)

 

DETERMINATION OF SPECIFIC ANTIBODIES TO ENTEROTOXINS
OF Staphylococcus aureus IN BLOOD AND COLOSTRUM FROM COWS

I.V. Loskutova1, 2, 3, M.P. Shchannikova1, 2, 3, K.K. Fursova1, 2,
A.O. Shepelyakovskaya1, 2, O.A. Artemyeva1, D.A. Nikanova1,
N.A. Zinovieva1, F.A. Brovko1, 2

1L.K. Ernst Federal Science Center for Animal Husbandry, Federal Agency of Scientific Organizations, 60, pos. Dubrovitsy, Podolsk District, Moscow Province, 142132 Russia,
e-mail vijmikrob@mail.ru (corresponding author);
2Pushchino Branch, M.M. Shemyakin–Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Federal Agency of Scientific Organizations, 6, Prospekt Nauki, Pushchino, Moscow Province, 142290 Russia;
3Pushchino State Institute of Natural Science, 3, Prospekt Nauki, Pushchino, Moscow Province, 142290 Russia


ORCID:
Loskutova I.V. orcid.org/0000-0002-4823-2121
Fursova K.K. orcid.org/0000-0001-9762-5901
Artemyeva O.A. orcid.org/0000-0001-7706-4182
Zinovieva N.A. orcid.org/0000-0003-4017-6863
Shchannikova M.P. orcid.org/0000-0003-2496-1167
Shepelyakovskaya A.O. orcid.org/0000-0001-8742-6191
Nikanova D.A. orcid.org/0000-0001-5164-244X
Brovko F.A. orcid.org/0000-0001-7498-774X

Received September 5, 2017

 

Mastitis is a widespread infectious disease of the milk cattle causing essential economic damage to livestock production and influencing quality of dairy products all over the world. The main pathogen of cattle mastitis is Staphylococcus aureus. S. aureus pathogenicity and steady persistence in the body are due to a set of toxins. The microorganism produces a number of pathogenic factors with an impact on character of the disease. At the moment there is no effective ways to fight against mastitis therefore research of the immune status of cows as per staphylococcus enterotoxins is under consideration. The aim of this study was identification and quantitation of Ig specific to the most widespread staphylococcus enterotoxins (A, B, C, D, E, G, H, I and TSST) in blood and in colostrum of Holstein black and motley cows of 2nd lactation (n = 47, Kaluga region, 2016). Animals were on loose housing keeping and two times a year exposed to immunization by vaccine MASTIVAK (Laboratorios Ovejero S.A., Spain). Anti-enterotoxin immunoglobulins were estimated using indirect enzyme immunoassay. In 53.19 % of blood samples IgG specific to SEH were detected. Antibodies to TSST were found in 4.26 % of samples, at the same time their amount was the lowest in comparison to the antibodies to other enterotoxins found. The analysis of colostrum and milk showed IgA to SEH, SEG and SEI in colostrum. In milk we did not revealed antibodies to enterotoxins. Thus, the antibodies to a wide range of staphylococcal enterotoxins (SEA, SEB, SEC, SED, SEE, SEG, SEH, SEI and TSST) we have found in blood and colostrum of clinically healthy cows could be indicative of animals’ contacts with the staphylococci producing these types of toxins.

Keywords: Staphylococcus aureus, mastitis in cows, blood, colostrum, enterotoxins, antibodies against enterotoxins.

 

Full article (Rus)

 

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