doi: 10.15389/agrobiology.2017.6.1279eng

UDC 636.086.1:636.085.19:632.4(470)

 

SPECIES COMPOSITION AND TOXICOLOGICAL CHARACTERISTICS
OF FUNGI OF THE GENUS Aspergillus ISOLATED FROM COARSE
FODDERS

G.P. Kononenko, E.A. Piryazeva, E.V. Zotova, A.A. Burkin

All-Russian Research Institute of Sanitary, Hygiene and Ecology, Federal Agency of Scientific Organizations, 5, Zvenigorodskoe sh., Moscow, 123022 Russia, 
e-mail kononenkogp@mail.ru (corresponding author)


ORCID:
Kononenko G.P. orcid.org/0000-0002-9144-615X
Zotova E.V. orcid.org/0000-0002-1479-8602
Piryazeva E.A. orcid.org/0000-0001-5443-3213
Burkin A.A. orcid.org/0000-0002-5674-2818

Received February 14, 2017

 

The problem of ensuring the safety of coarse fodders, which annually replenishes the Russian feed production on a large scale, raises concern about the multiple combined contamination with mycotoxins and the extensive spread of toxigenic fungi. Recently, it has been established that F. sporotrichioides plays a dominant role among fusarium fungi in these fodders, producing metabolites, the T-2 toxin and diacetoxyscirpenol, which can cause acute poisoning in animals. The purpose of this work, which became the next stage in the study of the main toxin-producing micromycetes of coarse fodders, was the determination of the species composition, occurrence and toxin production in fungi of the genus Aspergillus under experimental conditions favourable for the fullest realization of their potential. The objects of mycological analysis were 258 average samples from the production batches of hay and straw harvested in the livestock farms of Bryansk (2011) and Moscow (2013) regions. Isolates with established species affiliation were cultivated on Czapek-Dox agar (CDA), wort agar (WA) and moistened rice grain (RG) for 7 days at 23 °C. The content of sterigmatocystin (STE), emodin (EMO), aflatoxin B1 (AB1), ochratoxin A (OA), mycophenolic acid (MPA), cyclopiazonic acid (CPA), ergot alkaloids (EA), deoxynivalenol (DON) and fumonisins (FUM) in extracts of mycelial spore biomass were determined by enzyme immunoassay with certified test systems. To assess the toxin production, 32 isolates of 12 species of Aspergillus fungi of hay and straw were used, as well as 27 isolates of A. flavus Link, A. pseudoglaucus Blochwitz, A. repens de Bary isolated earlier from grain fodders. Fungi of the genus Aspergillus were found in samples with a frequency of 62.0 % and an infection rate of 1.7-100 %. The obtained isolates belonged to 15 species included in 10 taxonomic groups with the largest species diversity in the A. glaucus group (4 species). The most common species were A. flavus and A. niger van Tieghem (more than 50.0 % of the contaminated samples), followed by A. versicolor (Vuill.) Tiraboschi, A. pseudoglaucus, A. amstelodami (Mangin) Thom & Church, A. ochraceus Wilhelm and A. wentii Vehmer (10.6-18.8 %), A. nidulans Eidam (6.3 %), the remaining 7 species — A. candidus Link, A. tamarii Kita, A. sydowii (Bain. & Sart.) Thom & Church, A. fumigatus Fresenius, A. repens, A. terreus Thom, A. chevalieri (Mangin) Thom & Church were less common (< 5 %). The intensity of formation of the CPA (A. flavus) and MPA (A. pseudoglaucus, A. repens) was quite comparable in the CDA and RG. Compared to WA, a greater accumulation of the majority of mycotoxins occurred in the RG, i.e. STE (A. versicolor, A. nidulans), CPA (A. flavus, in all 5 A. tamarii isolates CPA could be detected only on this substrate) and EMO (A. sydowii). For the biosynthesis of MPA in A. pseudoglaucus and A. repens, WA was preferred. Testing of fungi on three nutrient media allowes us to establish that a complex of Aspergillus fungi which includes 7 species can be associated with the contamination of coarse fodders with STE, CPA and MPA; the source of EMO contamination among the fungi of the genus Aspergillus was not found. Only two of the three isolates of A. sydowii produced it in small amounts of 120±20 and 245±40 ng/g. The remaining mycotoxins analyzed in the isolates were not detected. The possibility of participation of fungi of other systematic groups in the contamination of fodder with STE, CPA, MPA and EMO is discussed, whereas clusters encoding the biosynthesis of mycotoxins have been found in micromycetes from genetically distinct groups in recent years.

Keywords: hay, straw, fungi of the genus Aspergillus, mycotoxins.

 

Full article (Rus)

Full article (Eng)

 

REFERENCES

  1. Steinbach W.J., Perfect J.R., Schell W.A., Walsh T.J., Benjamin D.K. In vitro analyses, animal models, and 60 clinical cases of invasive Aspergillus terreus infection. Antimicrob. Agents Chemother., 2004, 48(9): 3217-3225 CrossRef
  2. Sajid M.A., Khan I.A., Rauf U. Aspergillus fumigatus in commercial poultry flocks, a serious threat to poultry industry in Pakistan. J. Anim. Plant Sci., 2006, 16 (3-4): 79-81.
  3. Khmelevskii B.N., Pilipets V.I., Malinovskaya L.S., Kostin V.V., Komarnitskaya N.P., Ivanov V.G. Profilaktika mikotoksikozov zhivotnykh. [Prevention of animal mycotoxicosis]. Moscow, 1985 (in Russ.).   
  4. Gallo A., Giuberti G., Frisvad J.C., Bertuzzi T., Nielsen K.F. Review on mycotoxin issues in ruminants: occurrence in forages, effects of mycotoxin ingestion on health status and animal performance and practical strategies to counteract their negative effects. Toxins, 2015, 7: 3057-3111 CrossRef
  5. Greco M., Kemppainen M., Pose G., Pardo A. Taxonomic characterization and secondary metabolite profiling of Aspergillus Section Aspergillus contaminating feeds and feedstuffs. Toxins, 2015, 7: 3512-3537 CrossRef
  6. Abeer H., Abd-Allah E.F. Antifungal potential of propolis against carcinogenic citrinin produced by Aspergillus terreus Thom. J. Pure Appl. Microbio., 2014, 8(5): 3937-3943.
  7. Kononenko G.P., Burkin A.A. Mycotoxin contaminations in commercially used hay. Sel'skokhozyaistvennaya biologiya [Agricultural Biology], 2014, 4: 120-126 CrossRef (in Rus.).
  8. Kononenko G.P., Burkin A.A. Uspekhi meditsinskoi mikologii, 2014, 13: 305-306 (in Russ.).   
  9. Piryazeva E.A., Kononenko G.P., Burkin A.A. Affection of coarse fodders by toxigenic Fusarium fungi. Agricultural Biology, 2016, 51(6): 937-945 CrossRef (in Engl.).
  10. Raper K.B., Fennell D.J. The genus Aspergillus. Williams & Wilkins Company, Baltimore, 1965.
  11. Burkin A.A., Kononenko G.P. Mycotoxin contamination of meadow grasses in European Russia. Agricultural Biology, 2015, 50(4): 503-512 CrossRef (in Engl.).
  12. Lychagin G.Ya. Sbornik nauchnykh trudov Ryazanskogo SKHI, 1970, 24(2): 56-60 (in Russ.).   
  13. Nazypov M.N. Uchenye zapiski Kazanskogo veterinarnogo instituta, 1970, 104: 180-183 (in Russ.).   
  14. Tremasov M.Ya., Ivanov A.V., Sergeichev A.I., Titova V.Yu., Petro-
    va N.V. Materialy Mezhdunarodnogo simpoziuma «Agroekologicheskaya bezopasnost' v usloviyakh tekhnogeneza» [Proc. Int. Symp. «Environment-friendly agriculture in the era of thechnogenesis». Part 1]. Kazan', 2006, chast' 1: 358-362 (in Russ.).   
  15. Salikhova N.M. Trudy VNIIVS, 1973, 47: 43-46 (in Russ.).   
  16. Kaloev S.S. Trudy Kubanskogo sel'skokhozyaistvennogo instituta, 1975, 97(125): 3-7 (in Russ.).   
  17. Makarov Yu.A., Gorkovenko N.E. Dal'nevostochnyi agrarnyi vestnik, 2010, 4(16): 32-34 (in Russ.).   
  18. Starchenko L.E. Toksichnye griby na grubykh kormakh i ikh mikologicheskii kontrol' v usloviyakh L'vovskoi oblasti. Avtoreferat kandidatskoi dissertatsii [Toxic fungi Toxic fungi inhabiting coarse feeds and their control in L'vov region]. L'vov. 1967 (in Russ.).   
  19. Ivanov A.T. Sanitarno-mikologicheskie issledovaniya bobovykh kul'tur v usloviyakh Belorusskoi SSR. Avtoreferat kandidatskoi dissertatsii [Sanitary and mycological study of legumes in Belarussian SSR]. Moscow, 1967 (in Russ.).   
  20. Plauska V.A., Gustas A.P. Trudy Litovskogo NII veterinarii, 1969, 3: 118-125 (in Russ.).  
  21. Lugina Zh.A. Trudy VNIIVS, 1970, 35: 73-79 (in Russ.).   
  22. Lugina Zh.A., Kharatyan A.M. Izvestiya sel'skokhozyaistvennykh nauk, 1985, 1: 79-85 (in Russ.).   
  23. Zhakhanov A. Mikotoksikologicheskaya kharakteristika grubykh kormov i osobennosti toksinoobrazovaniya griba Fusarium sporotrichioides Sherb. na yuge Kazakhstana. Avtoreferat kandidatskoi dissertatsii [Mycotoxicology of coarse feeds and Fusarium sporotrichioidesSherb. toxin production in Southern Kazakhstan. PhD Thesis]. Alma-Ata. 1975 (in Russ.).   
  24. Ibragimov R.G. Mikoflora zernofurazha i vegetiruemykh kormovykh zlakov v yuzhnoi chasti Bol'shogo Kavkaza Azerbaidzhana i ikh toksikologicheskaya kharakteristika. Avtoreferat kandidatskoi dissertatsii [Mycoflora of grain forage and green fodder cereals, Big Caucasus, Azerbaijan, and their toxicological characterization. PhD Thesis]. Kiev, 1980 (in Russ.).   
  25. Azimov I.M., Askerov D.A., Ibragimov R.G., Gabiev M.M. V sbornike: Trudy Azerbaidzhanskogo nauchno-issledovatel'skogo veterinarnogo instituta: Infektsionnye i parazitarnye bolezni zhivotnykh v Azerbaidzhane [In: Animal infectious and parazirtic diseases in Azerbaijan. Proceedings of Azerbaijan Research Veterinary Institute]. Baku, 1984, 30: 96-100 (in Russ.).   
  26. Lu P., Zhao X., Cui T. Production of emodin from Aspergillus ochraceus at preparative scale. Afr. J. Biotechnol., 2010, 9(4): 512-517.
  27. Eroshkin A.A., Soboleva N.A., Burkin A.A., Kononenko G.P. V sbornike: Problemy veterinarnoi sanitarii i ekologii [In: Vetrinary sanitation and ecology — challenges. V. 1]. Moscow, 2000, tom 109: 134-144 (in Russ.).   
  28. Burkin A.A., Kononenko G.P., Kislyakova O.S. Uspekhi meditsinskoi mikologii, 2003, 1: 122-124 (in Russ.).   
  29. Kononenko G.P., Burkin A.A. Uspekhi meditsinskoi mikologii, 2007, 9: 88-89 (in Russ.). 
  30. Burkin A.A., Piryazeva E.A., Kononenko G.P., Malinovskaya L.S. Uspekhi meditsinskoi mikologii, 2006, 7: 94-95 (in Russ.).   
  31. Kononenko G.P., Burkin A.A. Mikologiya i fitopatologiya, 2008, 42(2): 178-184 (in Russ.).   
  32. Burkin A.A., Kononenko G.P. Producers of mycophenolic acid in ensiled and grain feeds. Appl. Biochem. Microbiol., 2010, (46)5: 545-550 CrossRef
  33. Frisvad J.C., Smedsgaard J., Samson R.A., Larsen T.O., Thrane U. Fumonisin B2 production by Aspergillus niger. J. Agric. Food Chem., 2007, 55(23): 9727-9732.
  34. Firouzmand R., Modiri L., Nosrati A.C. Deoxynivalenol production by Aspergillus spp. LAP Lambert Academic Publishing, Germany, Saarbrucken, 2013.
  35. Weidenbörner M. Encyclopedia of food mycotoxins. Springer, Berlin—Heidelberg—NY, 2001.
  36. Frisvad J.C., Rank C., Nielsen K.F., Larsen T.O. Metabolomics of Aspergillus fumigatus. Med. Mycol., 2008, 47: S1-S19 CrossRef
  37. Kononenko G.P., Burkin A.A. Immunopatologiya, allergologiya, infektologiya, 2010, 1: 195-196 (in Russ.).   
  38. Vinokurova N.G., Ivanushkina N.E., Khmel’nitskaya I.I., Arinbasa-
    rov M.U. Synthesis of a-cyclopiazonic acid by fungi of the genus Aspergillus. Appl. Biochem. Microbiol., 2007, 43(4): 486-489 CrossRef
  39. Kononenko G.P., Burkin A.A. Sovremennaya mikologiya v Rossii, 2015, 4(2): 201-203 (in Russ.).

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