Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2017, Vol. 52, № 6, p. 1094-1104
(how to cite this article)

doi: 10.15389/agrobiology.2017.6.1094eng

UDC 636.52/.58:637.4.04/.07

Acknowlegdgements:
Supported financially by Russian Science Foundation, grant № 16-16-04047.

 

BIOFORTIFICATION OF HEN EGGS: VITAMINS AND CAROTENOIDS (review)

A.Sh. Kavtarashvili1, V.M. Kodentsova2, V.K. Mazo1, D.V. Risnik1,
I.L. Stefanova1

1All-Russian Scientific Research Institute of Poultry Processing Industry — branch of Federal Scientific Center All-Russian Research and Technological Poultry Institute RAS, Federal Agency of Scientific Organizations, 1, Rzhavki, Solnechnogorsk Region, Moscow Province, 141552 Russia, e-mail dp.vniipp@mail.ru, alexk@vnitip.ru (corresponding author);
2Federal Research Centre of Nutrition and Biotechnology, Federal Agency of Scientific Organizations, 2/14, Ust’inskii pr., Moscow, 109240, Russia, e-mail: kodentsova@ion.ru

ORCID:
Kavtarashvili A.Sh. orcid.org/0000-0001-9108-1632
Kodentsova V.M. orcid.org/0000-0002-5288-1132
Mazo V.K. orcid.org/0000-0002-3237-7967
Risnik D.V. orcid.org/0000-0002-3389-8115
Stefanova I.L. orcid.org/0000-0002-4394-5149

Received June 14, 2017

 

The dependence of the vitamin content in the egg from the content in the chicken feed represents the saturation curves (V.M. Kodentsova et al., 2005; K. Hebert et al., 2005; S. Leeson et al., 2004; A.L. Shtele, 2004; S. Grobas et al., 2002). The effectiveness of the various forms of vitamins for enrichment of chicken feed and the use for this purpose of herbal supplements had been analyzed (P. Mattila et al., 2004; P.H. Mattila et al., 2011; M. Hammershøj et al., 2010, J.A. Moreno et al., 2016). The optimal content of vitamins and carotenoids in the poultry feed results in an increase to the maximum level of vitamins and carotenoids in the egg that makes them a significant source of vitamins D, E, B and carotenoid for humans. One such egg can provide up to 40-50 % of the recommended daily intake of vitamins D, B12, A, pantothenic acid, 30 % of vitamin E, 20 % of folate, 10 % of vitamin A, 12 % of vitamin B2, and up to 30 % of the adequate level of lutein intake. The advantage of biofortification is biotransformation in the chicken’s body of synthetic vitamins added to food into their natural form, which deprives the arguments of opponents of enrichment of food products with synthetic vitamins. Comparison of the addition of different forms of vitamins showed that D3 in the diet more effectively increased the vitamin content in egg yolk (P. Mattila et al., 2004). If the chicken feed contains vitamin D only as 25OHD3, then vitamin D in the form of cholecalciferol may be completely absent in the yolk (P.H. Matvila et al., 2011). Irradiation of chicken with ultraviolet light or free-range farming in the natural sunlight may provide an original, safe and natural alternative to produce vitamin D-enriched eggs (A. Schutkowski et al, 2013; J. Kühn et al., 2014, 2015) and chicken meat without the risk of overdose of this vitamin. By increasing the content of lutein in the yolk of a chicken egg, the bioavailability of this carotenoid can be substantially increased as compared to plant sources (G.J. Handelman et al., 1999). The enrichment of eggs with vitamins meets the criteria for the fortified foods (V.M. Kodentsova et al., 2010). Increasing the level of all vitamins in hen diet resulted in a simultaneous increase in the content of all vitamins in eggs (H. Zang et al., 2011). Biofortification has clear advantages over the technological enrichment since synthetic vitamins received from feed are converted into natural ones in hen body. Biofortification of eggs with vitamins is one of the most promising strategies to increase consumption of vitamins for population (M.S. Calvoa et al., 2013).

Keywords: biofortification, vitamins, сarotenoids, poultry, eggs.

 

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REFERENCES

  1. Kodentsova V.M. Pishchevaya promyshlennost', 2014, (3): 14-18 (in Russ.).   
  2. Vitamin compendium. The properties of the vitamins and their importance in human and animal nutrition. Basel, 1976.
  3. Applegate E. Introduction: nutritional and functional roles of eggs in the diet. J. Am. Coll. Nutr., 2000, 19(5): 495S-498S.
  4. Song W.O., Kerver J.M. Nutritional contribution of eggs to American diets. J. Am. Coll. Nutr., 2000, 19: 556S-562S.
  5. Kodentsova V.M., Vrzhesinskaya O.A., Beketova N.A., Kodentsova O.V. Voprosy pitaniya, 2005, 5: 19-24 (in Russ.).   
  6. Khimicheskii sostav rossiiskikh pishchevykh produktov /Pod redaktsiei I.M. Skurikhina, V.A. Tu-tel'yana [Chemical composition of Russian food stuffs. I.M. Skurikhin, V.A. Tutel'yan (eds.)]. Moscow, 2002 (in Russ.). 
  7. Khimicheskii sostav pishchevykh produktov /Pod redaktsiei I.M. Skurikhina, M.N. Volgareva [Chemical composition of food. I.M. Skurikhia, M.N. Volgarev (eds.)]. Moscow, 1987 (in Russ.).   
  8. Kirunda D.F., Scheideler S.E., Mckee S.R. The efficacy of vitamin E (DL-alpha-tocopheryl acetate) supplementation in hen diets to alleviate egg quality deterioration associated with high temperature exposure. Poultry Sci., 2001, 80: 1378-1383 CrossRef
  9. Cortinas L., Villaverde C., Galobart J., Baucells M.D., Codony R., Barroeta A.C. Fatty acid content in chicken thigh and breast as affected by dietary polyunsaturation level. Poultry Sci., 2004, 83: 1155-1164 CrossRef
  10. Zdunczyk Z., Gruzauskas R., Semaskaite A., Juskiewicz J., Racevici-ute-Stupeliene A., Wroblewska M. Fatty acid profile of breast muscle of broiler chickens fed diets with different levels of selenium and vitamin E. Arch. Geflugelkd., 2011, 75: 264-267.
  11. Chung M.K., Choi J.H., Chung Y.K., Chee K.M. Effects of dietary vitamins C and E on egg shell quality of broiler breeder hens exposed to heat stress. Asian-Australas. J. Anim. Sci., 2005, 18(4): 545-551 CrossRef
  12. Surai P.F. Natural antioxidants in avian nutrition and reproduction. UK, Nottingham, 2002.
  13. Karavashchenko V.F., Zhuk R.K., Pritulenko O.V. Materialy VI konferentsii baltiiskikh stran po ptitsevodstvu [Proc. VI Baltic Conf. on Poutlry Farming].Vil'nyus, 1998: 50-51 (in Russ.).   
  14. Mohiti-Asli M., Shariatmadari F., Loftollahian H., Mazuji M.T. Effects of supplementing layer hen diets with selenium and vitamin E on egg quality, lipid oxidation and fatty acid composition during storage. Can. J. Anim. Sci., 2008, 88: 475-483.
  15. Surai P.F. Vitamin E in avian reproduction. Poult. Avian Biol. Rev., 1999, 10: 1-60.
  16. Sergeeva A.M., Blagodatskikh A.V. Puti povysheniya kachestva pishchevykh yaits [Eggg quality improvement]. Moscow, 1978 (in Russ.).   
  17. Shtele A.L. Kurinoe yaitso: vchera, segodnya, zavtra [Chicken egg modifications]. Moscow, 2004 (in Russ.).   
  18. Jiang Y.H., McGeachin R.B., Bailey C.A. Alpha-tocopherol, beta-carotene, and retinol enrichment of chicken eggs. Poultry Sci., 1994, 73(7): 1137-1143.
  19. Sunder A., Halle I., Flachowsky G. Vitamin E hypervitaminosis in laying hens. Arch. Tierernahr., 1999, 52(2): 185-194.
  20. Handbook of vitamins. L.J. Machlin (ed.). NY, Basel, 1984.
  21. Eksperimental'naya vitaminologiya (spravochnoe rukovodstvo) /Pod redaktsiei Yu.M. Ostrovskogo [Experimantal vitaminology. Yu.M. Ostrovskii (ed.)]. Minsk, 1979 (in Russ.).   
  22. Mattila P., Valaja J., Rossow L., Venäläinen E., Tupasela T. Effect of vitamin D2- and D3-enriched diets on egg vitamin D content, production, and bird condition during an entire production period. Poultry Sci., 2004, 83: 433-440 CrossRef
  23. Vahteristo L.T., Ollilainen V., Varo P. Liquid chromatographic determination of folate monoglutamates in fish, meat, egg, and dairy products consumed in Finland. J. AOAC Int., 1997, 80(2): 373-378.
  24. Hebert K., House J.D., Guenter W. Effect of dietary folic acid supplementation on egg folate content and the performance and folate status of two strains of laying hens. Poultry Sci., 2005, 84(10): 1533-1538 CrossRef
  25. Hoey L., McNulty H., McCann E.M.E., McCracken K.J, Scott J.M, Marc B.B., Molloy A.M., Graham C., Pentieva K. Laying hens can convert high doses of folic acid added to the feed into natural folates in eggs providing a novel source of food folate. Br. J. Nutr., 2009, 101(2): 206-212 CrossRef
  26. Hammond B.R., Johnson E.J., Russell E.J., Krinsky N.I., Yeum K.J., Edwards R.B., Snodderly D.M. Dietary modification of human macular pigment density. Invest. Ophthamol. Vis. Sci., 1997, 38(9): 1795-1801.
  27. Leeson S., Caston L. Enrichment of eggs with lutein. Poultry Sci., 2004, 83(10): 1709-1712 CrossRef
  28. Meluzzi A., Sirri F., Manfreda G., Tallarico N., Franchini A. Effects of dietary vitamin E on the quality of table eggs enriched with n-3 long-chain fatty acids. Poultry Sci., 2000, 79: 539-545 CrossRef
  29. Imangulov Sh.A., Kavtarashvili A.Sh., Bebin M.L. Povyshenie kachestva yaits [Haw to improve egg quality]. Sergiev Posad, 1999 (in Russ.). 
  30. Hacquebard M., Carpentier Y.A. Vitamin E: absorption, plasma transport and cell uptake. Curr. Opin. Clin. Nutr., 2005, 8: 133-138.
  31. Galobart J., Barroeta A.C., Baucells M.D., Codony R., Ternes W. Effect of dietary supplementation with rosemary extract and alpha-tocopheryl acetate on lipid oxidation in eggs enriched with omega3-fatty acids. Poultry Sci., 2001, 80: 460-467.
  32. Barroeta A.C. Nutritive value of poultry meat: relationship between vitamin E and PUFA. World Poultry Sci. J., 2007, 63: 277-284 CrossRef
  33. Surai P., Fisinin V.I. Natural antioxidants in hens’ embryogenesis and antistress defence in postnatal development (review). Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2013, 2: 3-18 (in Engl.). 
  34. Sookwong P., Nakagawa K., Nakajima S., Amano Y., Toyomizu M., Miyazawa T. Tocotrienol content in hen eggs: its fortification by supplementing the feed with rice bran scum oil. Biosci. Biotech. Bioch., 2008, 72(11): 3044-3047 CrossRef
  35. Hammershøj M., Steenfeldt S. The effects of kale (Brassica oleracea ssp. acephala), basil (Ocimum basilicum) and thyme (Thymus vulgaris) as forage material in organic egg production on egg quality. Brit. Poultry Sci., 2012, 53(2): 245-256 CrossRef
  36. Kodentsova V.M., Risnik D.V. V sbornike: Ekologiya. Ekonomika. Informatika. Tom 1. Sistemnyi analiz i modelirovanie ekonomicheskikh i ekologicheskikh system [In: Ecology. Economy. Informatics. V. 1. System analysis and simulation of economic and environmental systems]. Rostov-na-Donu, 2016: 486-498 (in Russ.).   
  37. Calvoa M.S., Whiting S.J. Survey of current vitamin D food fortification practices in the United States and Canada. J. Steroid Biochem., 2013, 136: 211-213 CrossRef
  38. Mattila P.H., Valkonen E., Valaja J. Effect of different vitamin D supplementations in poultry feed on vitamin D content of eggs and chicken meat. J. Agric. Food Chem., 2011, 59: 8298-8303 CrossRef
  39. COMMISSION REGULATION (EC) No 887/2009 of 25 September 2009 concerning the authorisation of a stabilised form of 25-hydroxycholecalciferol as a feed additive for chickens for fattening, turkeys for fattening, other poultry and pigs. Available https://publications.euro-pa.eu/en/publication-detail/-/publication/57fd129b-5875-4ce7-aeb2-0539da4ed9ee/language-en. No date.
  40. Schutkowski A., Krämer J., Kluge H., Hirche F., Krombholz A., Theumer T., Stangl G.I. UVB exposure of farm animals: study on a food-based strategy to bridge the gap between current vitamin D intakes and dietary targets. PLoS ONE, 2013, 8(7): e69418 CrossRef
  41. Kühn J., Schutkowski A., Hirche F., Baur A.C., Mielenz N., Stangl G.I. Non-linear increase of vitamin D content in eggs from chicks treated with increasing exposure times of ultraviolet light. J. Steroid. Biochem., 2015, 148: 7-13 CrossRef
  42. Kühn J., Schutkowski A., Kluge H., Hirche F., Stangl G.I. Free-range farming: a natural alternative to produce vitamin D-enriched eggs. Nutrition, 2014, 30(4): 481-484 CrossRef
  43. Handelman G.J., Nightingale Z.D., Lichtenstein A.H., Schaefer E.J., Blumberg J.B. Lutein and zeaxanthin concentrations in plasma after dietary supplementation with egg yolk. Am. J. Clin. Nutr., 1999, 70: 247-251.
  44. Schaeffer J.L., Tyczkowski J.K., Parkhurst C.R., Hamilton P.B. Carotenoid composition of serum and egg yolks of hens fed diets varying in carotenoid composition. Poultry Sci., 1988, 67: 608-614.
  45. Surai P.F., Sparks N.H. Comparative evaluation of the effect of two maternal diets on fatty acids, vitamin E and carotenoids in the chick embryo. Brit. Poultry Sci., 2001, 42(2): 252-259 CrossRef
  46. Hammershøj M., Kidmose U., Steenfeldt S. Deposition of carotenoids in egg yolk by short-term supplement of coloured carrot (Daucus carota) varieties as forage material for egg-laying hens. J. Sci. Food Agr., 2010, 90(7): 1163-1171 CrossRef
  47. Hossein-nezhad A., Holick M.F. Vitamin D for health: A global perspective. Mayo Clin. Proc., 2013, 88(7): 720-755 CrossRef
  48. Akdemir F., Orhan C., Sahin N., Sahin K., Hayirli A. Tomato powder in laying hen diets: effects on concentrations of yolk carotenoids and lipid peroxidation. Brit. Poultry Sci., 2012, 53(5): 675-680 CrossRef
  49. Karadas F., Grammenidis E., Surai P.F., Acamovic T., Sparks N.H.C. Effects of carotenoids from lucerne, marigold and tomato on egg yolk pigmentation and carotenoid composition. Brit. Poultry Sci., 2006, 47: 561-566 CrossRef
  50. Jeon J.Y., Kim K.E., Im H.J., Oh S.T., Lim S.U., Kwon H.S., Moon B.H., Kim J.M., An B.K., Kang C.W. The production of lutein-enriched eggs with dietary chlorella. Kor. J. Food Sci. An., 2012, 32: 13-17 CrossRef
  51. Moreno J.A., Díaz-Gómez J., Nogareda C., Angulo E., Sandmann G., Portero-Otin M., Serrano J.C., Twyman R.M., Capell T., Zhu C., Christou P. The distribution of carotenoids in hens fed on biofortified maize is influenced by feed composition, absorption, resource allocation and storage. Scientific Reports, 2016, 6: 35346 CrossRef
  52. Tyczkowski J.K., Yagen B., Hamilton P.B. Metabolism of canthaxanthin, a red diketocarotenoid, by chickens. Poultry Sci., 1988, 67(5): 787-793.
  53. Haq A.U., Bailey C.A., Chinnah A. Effect of beta-carotene, canthaxanthin, lutein, and vitamin E on neonatal immunity of chicks when supplemented in the broiler breeder diets. Poultry Sci., 1996, 75(9): 1092-1097.
  54. Chow P.Y., Gue S.Z., Leow S.K., Goh L.B. The bioefficacy of microemulsified natural pigments in egg yolk pigmentation. Brit. Poultry Sci., 2014, 55(3): 398-402 CrossRef
  55. Zahroojian N., Moravej H., Shivazad M. Comparison of marine algae (Spirulina platensis) and synthetic pigment in enhancing egg yolk colour of laying hens. Brit. Poultry Sci., 2011, 52(5): 584-588 CrossRef
  56. Olson J.B., Ward N.E., Koutsos E.A. Lycopene incorporation into egg yolk and effects on laying hen immune function. Poultry Sci., 2008, 87(12): 2573-2580 CrossRef
  57. Chung H.Y., Rasmussen H.M., Johnson E.J. Lutein bioavailability is higher from lutein-enriched eggs than from supplements and spinach in men. J. Nutr., 2004, 134: 1887-1893.
  58. Sommerburg O., Keunen J.E., Bird A.C., vanKujik F.J. Fruits and vegetables that are sources for lutein and zeaxanthin: the macular pigment in human eyes. Br. J. Ophthalmol., 1998, 82: 907-910.
  59. European Food Safety Authority (EFSA). ESCO report on analysis of risks and benefits of fortification of food with folic acid. Appendix 2: EFSA meeting summary report. Folic acid: an update on scientific developments. Sweden, Uppsala, 2009: 94-115 CrossRef
  60. House J.D., Braun K., Ballance D.M, O’Connor C.P., Guenter W. The enrichment of eggs with folic acid through supplementation of the laying hen diet. Poultry Sci., 2002, 81: 1332-1337 CrossRef
  61. Benková J., Baumgartner J., Molnár F., Peškovicová D. Effect of folic acid addition into feed mixture of laying hens on its content in eggs. Slovak J. Anim. Sci., 2009, 42(3): 124-128.
  62. Sugiyama A., Awaji H., Horie K., Kim M., Nakata R. The beneficial effect of folate-enriched egg on the folate and homocysteine levels in rats fed a folate and choline-deficient diet. J. Food Sci., 2012, 77(12): 268-272 CrossRef
  63. Tercic D., Pestotnik M. Effect of excess folic acid on egg production, fertility and hatchability in layer breeders. Acta Agraria Kaposváriensis, 2014, 18 (Suppl. 1): 122-128.
  64. Grobas S., Mendez J., Lopez B.C., De B.C., Mateos G.G. Effect of vitamin E and A supplementation on egg yolk alpha-tocopherol concentration. Poultry Sci., 2002, 81(3): 376-381.
  65. Surai P.F., Ionov I.A., Kuklenko T.V., Kostjuk I.A., Acpherson A.M., Speake B.K., Noble R.C., Sparks N.H.C. Effect of supplementing the hen's diet with vitamin A on the accumulation of vitamins A and E, ascorbic acid and carotenoids in the egg yolk and in the embryonic liver. Brit. Poultry Sci., 1998, 39(2): 257-263 CrossRef
  66. Surai A.P., Surai P.F., Steinberg W., Wakeman W.G., Speake B.K., Sparks N.H.C. Effect of canthaxanthin content of the maternal diet on the antioxidant system of the developing chick. Brit. Poultry Sci., 2003, 44(4): 612-619 CrossRef
  67. Zang H., Zhang K., Ding X., Bai S., Hernández J.M., Yao B. Effects of different dietary vitamin combinations on the egg quality and vitamin deposition in the whole egg of laying hens. Rev. Bras. Cienc. Avic., 2011, 13(3): 189-196 CrossRef
  68. Park S.W., Namkung H., Ahn H.J., Paik I.K. Enrichment of vitamins D3, K and iron in eggs of laying hens. Asian Austral. J. Anim., 2005, 18(2): 226-229.
  69. Kodentsova V.M. Meditsinskii sovet, 2016, (9): 106-114 (in Russ.).   
  70. Kodentsova V.M., Vrzhesinskaya O.A., Spirichev V.B., Shatnyuk L.N. Voprosy pitaniya, 2010, 79(1): 23-33 (in Russ.).

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