Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2017, Vol. 52, № 6, p. 1184-1193
(how to cite this article)

doi: 10.15389/agrobiology.2017.6.1184eng

UDC 636.11:591.1

Acknowledgements:
The samples of cryopreserved semen were got from Bioresource Collection «Cryobank of Genetic Resources», ARRIHB.
Supported financially by Russian Science Foundation (project 17-16-01109) and the program of Federal Agency of Scientific Organizations for bio resource collections

 

REPRODUCTIVE FUNCTION IN PUREBRED ARABIAN STALLIONS
AS RELATED TO THE LEVELS OF CHEMICAL ELEMENTS IN MANE
HAIR SAMPLES

V.A. Bagirov1, V.V. Kalaschnikov2, A.M. Zaitsev2, M.M. Atroshchenko2,
S.A. Miroshnikov3, 4, O.A. Zavialov3, A.N. Frolov3

1L.K. Ernst Federal Science Center for Animal Husbandry, Federal Agency of Scientific Organizations, 60, pos. Dubrovitsy, Podolsk District, Moscow Province, 142132 Russia,
e-mail vugarbagirov@mail.ru;
2All-Russian Research Institute for Horse Breeding, Federal Agency of Scientific Organizations, pos. Divovo, Rybnovskii Region, Ryazan Province, 391105 Russia,
e-mail atromiks-77@mail.ru, vniik08@mail.ru, amzaitceff@mail.ru (corresponding authors);
3All-Russian Research Institute of Beef Cattle Breeding, Federal Agency of Scientific Organizations, 29, ul. 9 Yanvarya, Orenburg, 460000 Russia, e-mail vniims.or@mail.ru, Oleg-Zavyalov83@mail.ru, Forleh@mail.ru;
4Orenburg State University, 13, prosp. Pobedy, Orenburg, 460018 Russia


ORCID:
Bagirov V.A. orcid.org/0000-0001-5398-8815
Zaitsev A.M. orcid.org/0000-0003-4260-602X
Miroshnikov S.A. orcid.org/0000-0002-5746-9569
Frolov A.N orcid.org/0000-0003-4525-2554
Kalaschnikov V.V. orcid.org/0000-0001-9845-1691
Atroshchenko M.M. orcid.org/0000-0001-6023-0332
Zavialov О.А. orcid.org/0000-0003-2033-3956

Received September 29, 2017

 

In modern animal husbandry, along with the improvement of reproductive technologies, it is becoming increasingly important to restore the natural fertility of livestock, in particular breeding producers. Reproductive function is one of the most sensitive that reacts to changes in biogeochemical and environmental parameters. In this regard, research aimed at studying effects of the level of chemical elements in the body on reproductive function in stallions is rather important. The parameters of sperm quality depending on the level of chemical elements in hair sampled from mane were studied. In this work we used biomaterial of purebred Arabian stallions (n = 50) of the same biogeochemical province. Reproductive qualities of stallions were evaluated in relation to the pool of chemical elements rated by concentration in the mane hair. All animals were grouped according to the levels of individual elements in hair (i.e. up to percentile 25, within the interval of percentiles 25-75 and above percentile 75). Also the quality of fresh and cryopreserved semen was estimated. Hair profiles was determined for 25 elements (Al, As, B, Ca, Cd, Co, Cr, Cu, Fe, I, K, Li, Mg, Mn, Na, Ni, P, Pb, Se, Si, Sn, Hg, Sr, V, Zn) by inductively coupled plasma atomic emission and mass spectrometry (ICP-AES and ICP-MS). Ejaculate was assessed for volume, concentration, total spermatozoa number, number of spermatozoa with progressive motility and semen viability. The validity of the differences was verified by Mann-Whitney U test. It has been found out that the mane hair analysis can be used to detect reduced fertility in stallions. Increase in average values of Sr to 4.19±0.12, Se to 0,559±0.015, B to 21.55±1.14 µg/g in hair results in decrease in number and activity of sperm and filtrate volume. Activity and survival of stallion spermatozoa after thawing was in inverse correlation with Pb and Sn pool in the body, as determined by element level in the mane hair. With the increase in the concentration of these elements to 0,806±0206 and 0,051±0.008 mg/g, the sperm activity declines after thawing by 3.8-7.7 % and survival by 26.4-29.5 %, respectively. Animals with copper, silicon and vanadium in hair less than that of percentile 25, had the most active sperm. The survival rate of fresh sperm was associated with V concentration in hair being the highest in animals with low levels of this mineral. Thus, the assay of mane hair mineral profile can be used in monitoring for timely correction of stallions’ fertility.

Keywords: purebred horses, Arabian breed, stallion, profiles of mineral elements in the horse hair, reproductive function, sperm quality.

 

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