Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2016, V. 51, № 6, pp. 775-781.

doi: 10.15389/agrobiology.2016.6.775eng

UDC 636.2.034:636.082:575.162

Acknowledgements:
Supported by Federal Agency of Scientific Organizations and Ministry of Education and Science of the Russian Federation (project № 14.604.21.0062, RFMEFI60414X0062)

 

ASSOCIATIONS OF BoLA-DRB3 GENOTYPES WITH BREEDING VALUES FOR MILK PRODUCTION TRAITS IN RUSSIAN DAIRY CATTLE POPULATION

A.A. Sermyagin1, N.V. Kovalyuk2, A.N. Ermilov1, I.N. Yanchukov1,
V.F. Satsuk2, A.V. Dotsev1, T.E. Deniskova1, G. Brem1, 3,
N.A. Zinovieva1

1L.K. Ernst All-Russian Research Institute of Animal Husbandry, Federal Agency of Scientific Organizations, 60, pos. Dubrovitsy, Podolsk District, Moscow Province, 142132 Russia, e-mail alex_sermyagin85@mail.ru;
2North-Caucasian Institute of Animal Production, Federal Agency of Scientific Organizations, 4, ul. Pervomayskaya, pos. Znamenskii, Krasnodar, 350055 Russia,
e-mail nvk1972@yandex.ru;
3Institut für Tierzucht und Genetik, University of Veterinary Medicine (VMU), Veterinärplatz, A-1210, Vienna, Austria, e-mail gottfried.brem@agrobiogen.de

Received September 14, 2016

 

Breeding for genetically resistant cattle is an attractive approach to eradicate infectious disease in livestock. Bovine leukaemia is one of the most common infectious diseases of dairy cattle, which causes significant economic losses. Numerous studies have demonstrated that polymorphisms in the DRB3 gene at thebovine leukocyte antigen (BoLA) locus are associated with cow resistance to persistent lymphocytosis (PL), a clinical sign of leukaemia. Alleles associated with PL susceptibility are generally prevalent in dairy cattle populations and may reflect their association with milk productivity. Our objective was to evaluate the associations between genotypes at the BoLA-DRB3 locus and milk production traits in dairy cattle in Russia. In total, 171 Holstein sires were genotyped for DRB3 using the PCR-RFLP technique. Detected BoLA-DRB3 alleles were assigned to three categories according to their PL susceptibility. The DRB3.2*08, DRB3.2*16, DRB3.2*22 and DRB3.2*24 alleles were defined as susceptible (S), whereas alleles DRB3.2*11, DRB3.2*23 and DRB3.2*28 were identified as resistant (R), and the remaining alleles were denoted as neutral (N). We calculated the estimated breeding values (EBV) for milk production traits, including milk yield (MY), fat yield (FY) and protein yield (PY), using genotype categories at the BoLA-DRB3 locus as a fixed effect. We detected fifteen alleles, forty-nine genotypes and six genotype categories for the BoLA-DRB3 gene. The PL-susceptibility alleles were prevalent in Russian dairy sires (ranging from 0.0877 for BoLA-DRB3.2*08 to 0.2135 for BoLA-DRB3.2*22), assuming 0.6258. The bulls carrying the SS genotype category were characterized by significantly higher EBVs for MY and PY compared to the other genotype categories: 89.3 kg for MY (p < 0.001 vs. NN, NR, SN, p < 0.01 vs. SR, p < 0.05 vs. RR), 2.15 kg for FY (p < 0.001 vs. NN, NR, p < 0.05 vs. SN); 4.81 kg for PY (p < 0.001 vs. all other genotype categories). Our findings indicate that high frequencies of PL-susceptibility alleles in BoLA-DRB3 may be a result of their association with milk production traits in dairy cattle in Russia. Therefore, to prevent the development of clinical forms of bovine leukaemia in dairy herds in Russia, the impact of the genotypes at the BoLA-DRB3 gene locus on milk production traits must be considered.

Keywords: BoLA-DRB3 genotypes, BLV susceptible alleles, milk production traits, genetically resistant cattle.

 

Full article (Rus)

Full text (Eng)

 

REFERENCES

  1. Ott S.L., Johnson R., Wells S.J. Association between bovine-leukosis virus seroprevalence and herd-level productivity on US dairy farms. Prev. Vet. Med., 2003, 61: 249-262 CrossRef
  2. Nekouei O., Van Leeuwen J., Sanchez J., Kelton D., Tiwari A., Keefe G. Herd-level risk factors for infection with bovine leukemia virus in Canadian dairy herds. Prev. Vet. Med., 2015, 119: 105-113 CrossRef
  3. Ivanov O.V., Ivanova O.Yu., Fedotov V.P., Balandina M.V., Verkhovskii O.A., FedorovYu.N. Age dynamics in number of antibodies against leucosis virus in calves born from seropositive cows. Agricultural Biology, 2008, 6: 87-90 (in Engl.).
  4. Sigurdardottir S., Lunden A., Andersson L. Restriction fragment length polymorphism of DQ and DR class II genes of the bovine major histocompatibility complex. Anim. Genet., 1988, 19: 133-150 CrossRef
  5. Xu A.L., Vaneijk M.J.T., Park C., Lewin H.A. Polymorphism in BoLA-DRB3 exon 2 correlates with resistance to persistent lymphocytosis caused by bovine leukemia virus. J. Immunol., 1993, 151: 6977-6985.
  6. Kelm S.C., Deitilleux J.C., Freeman A.E., Kehrli M.E. Jr., Dietz A.B., Fox L.K., Butler J.E., Kasckovics I., Kelley D.H. Genetic association between parameters of innate immunity and measures of mastitis in preparturient Holstein cattle. J. Dairy Sci., 1997, 80: 1767-1775.
  7. Lewin H.A., Russell G.C., Glass E.J. Comparative organization and function of the major histocompatibility complex of domesticated cattle. Immunol. Rev., 1999, 167: 145-158 CrossRef
  8. Juliarena M.A., Poli M., Sala L., Ceriani C., Gutierrez S., Dolcini G., Rodríguez E.M., Mariño B., Rodríguez-Dubra C., Esteban E.N. Association of BLV infection profiles with alleles of the BoLA-DRB3.2 gene. Anim. Genet., 2008, 39(4): 432-438 CrossRef
  9. Forletti A., Juliarena M.A., Ceriani C., Amadio A.F., Esteban E., Gutiérrez S.E. Identification of cattle carrying alleles associated with resistance and susceptibility to the Bovine Leukemia Virus progression by real-time PCR. Res. Vet. Sci., 2013, 95(3): 991-995 CrossRef
  10. Behl J.D., Verma N.K., Tyagi N., Mishra P., Behl R., Joshi B.K. The major histocompatibility complex in bovines: a review. ISRN Vet. Sci., 2012, Article ID 872710 CrossRef
  11. Sharif S., Mallard B.A., Wilkie B.N., Sargeant J.M., Scott H.M., Dekkers J.C., Leslie K.E.Associations of the bovine major histocompatibility complex DRB3 (BoLA-DRB3) alleles with occurrence of disease and milk somatic cell score in Canadian dairy cattle. Anim. Genet., 1998, 29: 157-160.
  12. Ledwidge S.A., Mallard B.A., Gibson J.P., Jansen G.B., Jiang Z.H. Multi-primer target PCR for rapid identification of bovine DRB3 alleles. Anim. Genet., 2001, 32: 219-221.
  13. Pashmi M., Qanbari S., Ghorashi S.A., Sharifi A.R., Simianer H. Analysis of relationship between bovine lymphocyte antigen DRB3.2 alleles, somatic cell count and milk traits in Iranian Holstein population. J. Anim. Breed. Genet., 2009, 126: 296-303 CrossRef
  14. Kovaljuk N.V., Satsuk V.F., Matviets A.V., Machulskaja E.V. Possible causes and consequences of the spread of individual allelic variants of the BoLA–DRB3 locus in groups of Holstein and Ayrshire cattle. Russ. J. Genet., 2010, 46(3): 379-382.
  15. Oprzadek J., Urtnowski P., Sender G., Pawlik A., Lukaszewicz M. Frequency of BoLA-DRB3 alleles in Polish Holstein-Friesian cattle. Anim. Sci. Paper Rep., 2012, 30(2): 91-101.
  16. Starkenburg R.J., Hanses L.B., Kehrli Jr.M.E., Chester-Jones H. Frequencies and effects of alternative DRB3.2 alleles of bovine lymphocyte antigen for Holsteins in milk selection and control lines. J. Dairy Sci., 1997, 80: 3411-3419 CrossRef
  17. Sharif S., Mallard B.A., Wilkie B.N., Sargeant J.M., Scott H.M., Dekkers J.C., Leslie K.E. Associations of the bovine major histocompatibility complex DRB3 (BoLA-DRB3) with production traits in Canadian dairy cattle. Anim. Genet., 1998, 30(2): 157-160 CrossRef
  18. Rupp R., Hernandez A., Mallard B.A. Association of bovine leukocyte antigen (BoLA) DRB3.2 with immune response, mastitis, and production and type traits in Canadian Holstein. J. Dairy Sci.,2007, 90: 1029-1038 CrossRef
  19. Duangjinda M., Buayai D., Pattarajinda V., Phasuk Y., Katawatin T., Vongpralub T., Chaiyotvittayakul A. Detection of bovine leukocyte antigen DRB3 alleles as candidate markers for clinical mastitis resistance in Holstein × Zebu. J. Anim. Sci., 2009, 87: 469-476 CrossRef
  20. Van Eijk M.J.T., Stewart-Haynes J.A., Lewin H.A. Extensive polymorphism of the BoLA-DRB3 gene distinguished by PCR-RFLP. Anim. Genet., 1992, 23: 483-496 CrossRef
  21. Yoshida T., Mukoyama H., Furuta H., Kondo Y., Takeshima S.N., Aida Y., Kosugiyama M., Tomogane H. Association of BoLA-DRB3 alleles identified by a sequence-based typing method with mastitis pathogens in Japanese Holstein cows. Anim. Sci. J., 2009, 80(5): 498-509 CrossRef
  22. Wu X.X., Yang Z.P., Wang X.L., Mao Y.J., Li S.C., Shi X.K., Chen Y. Restriction fragment length polymorphism in the exon 2 of the BoLA-DRB3 gene in Chinese Holstein of the south China. J. Biomed. Sci. Eng., 2010, 3: 221-225CrossRef
  23. Gladyr' E.A., Zinov'eva N.A., Bykova A.S., Vinogradova I.V., Ernst L.K. Dostizheniya nauki i tekhniki APK, 2012, 8: 46-48 (in Russ.).

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