doi: 10.15389/agrobiology.2013.6.36eng
UDC 636.52/.58:619:578.82/.83(470)
ABOUT SPREADIANG THE AVIAN LEUKOSIS VIRUSES IN POULTRY FARMS IN RUSSIAN FEDERATION
V.A. Plotnikov1, T.V. Grebennikova1, E.K. Dudnikova1, M.I. Shulpin2, S.P. Lazareva2, Z.B. Nikonova2, A.E. Menshikova2, S.N. Norkina1, T.I. Aliper1
1D.I. Ivanovsky Research Institute of Virology,
16, ul. Gamalei, Moscow, 123098 Russia,
e-mail: plotnvadim@yandex.ru, t_grebennikova@mail.ru,
dudnikova@narvac.com, snork9@list.ru;
2Federal Center for Animal Health Control,
FGBU VNIIZZh, mkr. Yurievets, Vladimir, 600901 Russia,
e-mail: shulpin@arriah.ru
Received September 10, 2013
In 2005-2011, in 46 administrative regions of the Russian Federation the monitoring of 223 poultry farms (broiler and egg production, and also the breeding farms) has been carried out to detect Avian Leukosis Virus (ALV) and antibodies to ALV subgroup J using PCR and ELISA. A total number of samples tested was more then 10,000. Antibodies to ALV and ALV subgroup J were identified at 90 and 70 % of examined farms (for broiler production as well as egg production), respectively. ALV subgroup J was found at breeding farms and egg farms (the Hysex, Rodonit, Ross 308 lines). Twelve Russian ALV field isolates have been obtained, and their molecular genetic properties were characterized. The field isolates formed two phylogenetic groups. In the first group the endogenous and exogenous recombinant VLP which are being isolated in the United States, China, France and Japan since 1993 are represented. The second phylogenetic group consists of ALV subgroup J isolates obtained in China, France and the United States. Each group contains the Russian isolates we obtained.
Keywords: avian leukosis virus, ELISA, PCR.
REFERENCES
1. Coffin J.M. Structure and classification of retroviruses. The Retroviridae. Plenum Press, N.Y., 1992. Vol. 1: 19-49.
2. Payne L.N., Fadly A.M. Leukosis/sarcoma group. In: Diseases of Poultry /B.W. Calnek, H.J. Barnes, C.W. Beard, L.R. Mc Dougald, Y.M. Saif (eds.). Iowa, 1997. Vol. 10: 414-466.
3. Ewert D.L., DeBoer G.F. Avian lymphoid leukosis: Mechanism of lymphomagenesis. Immunodeficiency Disorders. Boston, 1988: 37-53.
4. Fadly A.M., Smith E.J. Isolation and some characteristics of a subgroup J-like avian leukosis virus associated with myeloid leukosis in meat-type chickens in the United States. Avian Dis., 1999, 43: 391-400.
5. Payne L.N., Brown S.R., Bumstead N., Howes K., Frazier J.A., Thouless M.E. A novel subgroup of exogenous avian leukosis virus in chickens. J. Gen. Virol., 1991, 72: 801-807.
6. Payne L.N., Gillespie A.M., Howes K. Induction of myeloid leukosis and other tumors with the HPRS-103 strain of ALV. Vet. Rec., 1991, 129: 447-448.
7. Payne L.N., Gillespie A.M., Howes K. Myeloid leukemia and transmission of the HPRS-103 strain of avian leukosis virus. Leukemia, 1992, 6: 1167-1176.
8. Crittenden L.B. Exogenous and endogenous leukosis virus genes — A review. Avian Pathology, 1981, 10: 101-112.
9. Gavora J.S. Influence of avian leukosis virus infection on production and mortality and the role of genetic selection in the control of lymphoid leukosis. In: Avian leukosis /G.F. de Boer (ed.). Martinus Nijhoff Puplishing, Boston, 1986: 241-260.
10. Grebennikova T.V., Alekseev K.P., Vlasova A.N., Pokrovskaya M.A., Bogos'yan A.A., Zaberezhnyi A.D., Aliper T.I., Nepoklonov E.A. Ptitsa i ptitseprodukty, 2003, 6: 35-37.
11. Plotnikov V.A., Grebennikova T.V., Dudnikova E.K., Norkina S.N., Aliper T.I. Voprosy virusologii, 2012, 5: 38-43.
