doi: 10.15389/agrobiology.2013.6.105eng

UDC 636.086.2/.3:577.112(476)

BIOCHEMICAL PECULIARITIES OF Galega orientalis Lam. GENOTYPES IN BELORUSSIA

V.I. Domash, V.N. Prokhorov, O.L. Kandelinskaya, T.P. Sharpio, S.A. Zabreiko, E.R. Grischenko

V.F. Kuprevich Institute of Experimental Botani, National Academy of Sciences of Belorussia,
27, ul. Akademicheskaya, Minsk, 220072 Belarus Republic,
e-mail: domash@biobel.bas-net.by, valdomash@mail.ru, bot277@biobel.bas-net.by

Received February 6, 2012


Galega orientalis Lam. is a fodder crop with a number of important traits, including the early beginning of vegetation, fast growth, high productivity and nutritiousness. For its cultivation, the doses of mineral N are reduced due to active symbiotic N-fixation, resulting in the decrease of nitrogen content in the fodder and livestock production. Lectins and proteinase inhibitors, as anti-nutrients, affect the fodder quality negatively. But the distribution and activity of proteolytic factors and lectins in Galega orientalis are not still clarified enough. We conducted the chemical investigation of leaves and seeds in 19 varieties and samples of Galega orientalis, originated mainly from the Eastern Europe. The activity of neutral and alkaline proteinases, tripsin inhibitors, lectin phytohemagglutination (PHA), as well as the protein content, composition and digestibility were examined. In total, the parameters varied significantly. The tested Galega varieties are the highly valuable fodder crop. Particularly, the content of proteins and essential amino acids in plants was high. The amino acid composition, necessary for the young growing animals, was revealed. In contrary, the content of tripsin inhibitors and lectins was not sufficient. The inhibitors were more active in the plants which began their vegetation later, and PHA was higher in the plants with early and intermediate beginning of vegetation. The characteristic electrophoretic patterns of seed proteins were also shown.

Keywords: goat's rue, galega, fodder galega, eastern galega, trypsin inhibitors, lectins, proteins, aminoacids.

 

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REFERENCES

1. Laman  N.A.,  Prokhorov  V.N.,  Morozova  I.M.,  Sakovskaya  A.G. Galega  vostochnaya — mnogoletnyaya vysokobelkovaya kormovaya kul'tura: biologicheskie osobennosti, tekhnologiya vozdelyvaniya, khozyaistvennoe ispol'zovanie [Galega orientalis, a perennial high-protein forage crop: biology, cultivation technology, practical use]. Minsk, 2008.
2. Yaroshevich  M.I.,  Kukhareva  L.V.,  Boreisha  N.S. Galega vostochnaya — perspektivnaya kormovaya kul'tura. Biologiya, kormovaya tsennost', trebovaniya k usloviyam proizrastaniya, osobennosti vozdelyvaniya [Galega orientalis as a promising fodder crop. Biology, nutritive value, particular requirements to growth conditions and cultivation technology]. Minsk, 1991.
3. Kshnikatkina  A.N.,  Gushchina  V.A.,  Galiullin  A.A. Sel’skokhozyaistvennaya Biologiya [Agricultural Biology],  2003, 2: 101-107.
4. Strel'tsina  S.A., Yudcevich  E.V.,  Zhukova  M.A,  Konarev A.V.,  Dzyubenko  N.I. Agrarnaya Rossiya, 2006, 6: 46-52.
5. Tokar'  N.A. Novye i malorasprostranennye kormosilosnye rasteniya [New and rare fodder crops]. Kiev, 1969: 149-152.
6. Zevakhina  Yu.A.,  Ofitserov  E.N. Khimiya rastitel'nogo syr'ya, 2003, 2: 33-38.     
7. Strel'tsina  S.A.,  Zhukova  M.A.,  Chachko E.V.,  Dzyubenko  N.I.,  Konarev  A.V. Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2001, 5: 37-47.
8. Sagirova  R.A. Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2009, 4: 75-80.
9. Mosolov  V.V.,  Grigor'eva  L.I.,  Valueva  T.A. Prikladnaya biokhimiya i mikrobiologiya, 2001, 37(7): 131-140.
10. Domash  V.I.,  Sharpio  T.P.,  Zabreiko  S.A.,  Sosnovskaya  T.F. Bioorganicheskaya khimiya, 2008, 34(3): 353-357.
11. Shakirova  F.M.,  Bezrukova  M.V. Zhurnal obshchei biologii, 2007, 68(2): 109-125.
12. Ponomarenko  Yu.A. Pitatel'nye i antipitatel'nye veshchestva v kormakh [Nutritive and anti-nutritive components in forage]. Minsk, 2007.
13. Gofman  Yu.Ya.,  Vaisblai  I.M. Prikladnaya biokhimiya i mikrobiologya, 1975, 11(5): 777-787.
14. Anson  M.Z. The estimation of pepsin, trypsin, papain and catepsin with hemoglobin. J. Genet. Physiol., 1938, 22: 79-89.
15. Erlanger  F.,  Kokowsky  N.,  Cohen  W. The preparation and properties of two new chromogenic substrates of trypsin. Arch. Biochem. Biophys., 1961, 96: 271-278.
16. Sytnikov  D.M.,  Kots'  S.Ya.,  Malichenko  S.M. Fiziologiya i biokhimiya kul'turnykh rastenii, 2006, 38, 1: 53-60.
17. Babosha  A.V.,  Ladygina  M.E. Fiziologo-biokhimicheskie i biofizicheskie metody diagnostiki stepeni ustoichivosti rastenii k patogenam i drugim faktoram /Pod reaktsiei M.E. Ladyginoi [Physiobiochemical and biophysical methods for diagnostics of plant resistance to pathogens and other factors. M.E. Ladynina (ed.)]. Moscow, 1992: 43-52.
18. Levitskii  A.P.,  Pokhomenko  L.I.,  Vovchuk  S.V. Biokhimicheskii metod opredeleniya perevarimosti zernovykh belkov: Metodocheskie rekomendatsii [Biochemical estimation of grain proteins digestibility]. Odessa, 1985.
19. Bradford  M.M. Rapid and sensitive method for quantitation of microgramm quantities of protein utilizing the principle of protein dye binding.
Anal. Biochem., 1976, 8: 248-254.
20. Laemmli  U.K. Cleavage of structure protein during the assembly of the head of bacteriophage. Nature, 1970, 227(5259): 680-685.
21. Lokshina  L.A. Molekulyarnaya biologiya, 1979, 13(6): 1205-1229.
22. Mosolov  V.V.,  Valueva  T.A. Prikladnaya biokhimiya i mikrobiologiya, 2005, 41(3): 261-283.
23. Lenindzher  A. Osnovy biokhimii. Tom 2 [Principles of biochemistry]. Moscow, 1985, Vol. 2: 653-681.

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