Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2012, № 6, p. 56-60.

doi: 10.15389/agrobiology.2012.6.56eng

UDK 636.52/.58:573.6.086.83:636.082

OPTIMIZATION OF CONDITIONS FOR RECOMBINANT DNA INJECTION INTO SPERMATOGENIC CELLS OF THE CHICKEN in vivo

N.A. Volkova¹, L.А. Volkova¹, I.K. Fomin¹, N.A. Zinovieva¹, N.S. Lotsmanova²

The factors affecting the efficiency of the transfer of recombinant DNA into seminiferous epithelium of the chicken in vivo using retroviral vectors are studied. The maximum efficiency of the transformation of spermatogenic cells in chicken is demonstrated when virus was used as a source of genetic structure (pLgfpSN gene construct which contains the reporter gene of green fluorescent protein GFP based on the recombinant retroviral vector). The possibility of transmission of the transgene to offspring with the efficiency of transgenesis 4.2 % is shown.

Keywords: transgenesis, retrovirus vectors, spermatogonia cells, chicken.

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