Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2012, № 6, p. 66-72.

doi: 10.15389/agrobiology.2012.6.66eng

UDK 619+636/639]:576.3/.7.086.83:591.04

in vitro MYOGENIC DIFFERENTIATION OF BOVINE MULTIPOTENT MESENCHYMAL STEM CELLS TAKEN FROM BONE MARROW AND ADIPOSE TISSUE

I.A. Rogov¹, I.M. Volkova^{1, 2}, K.V. Kuleshov², I.P. Savchenkova²

Bovine bone marrow (BM) and adipose tissue (AT) multipotent mesenchymal stem cells (MMSC) are believed to be a perspective material for the creation of new cellular systems and products for food biotechnology. We demonstrated the possibility of directed differentiation of BM and AT MMSC in those of muscle tissue by culturing them in the medium, containing inductors of myogenesis (5-azacytidine, 5-aza-2'-deoxycytidine and retinoic acid). The comparative analysis of differentiation efficiency of these cells in the direction of myogenesis revealed morphological changes in BM MMSC on the 15-21st day, and in AT MMSC — on the 20-25th day of culturing in the medium with different inductors. The analysis of MYOD1 and MYOG gene expression in RT-PCR detected essential distinctions in the potency of these cells to differentiate into muscle tissue cells in vitro, and also showed that MMSC, isolated from BM, are more perspective source for muscle tissue engineering in vitro.

Keywords: multipotent mesenchymal stem cells, bone marrow, adipose tissue, bovine, differentiation, myogenesis, in vitro, inductors, gene expression.

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