doi: 10.15389/agrobiology.2012.6.109eng

UDK 636/639:614.31:619

METHODS FOR SANITARY SURVEILLANCE OF LIVESTOCK PRODUCTION. VII. ENZYMOIMMUNOASSAY OF STREPTOMYCIN

M.A. Burkin1, I.A. Gal'vidis1, G.P. Kononenko2

The polyclonal rabbit antibodies against streptomycin conjugated with bovine serum albumin in reaction with diglycidyl ether of 1,4-butanediol presents high specificity to streptomycin and its similar structural analog — dihydrostreptomycin. In the conditions of indirect competitive enzymoimmunoassay with immobilized antigens, heterologous to immunogene on protein carrier and methods of synthesis, the limit of streptomycin detection is 0.1 ng/ml. The authors consider the use of developed immunoenzyme test-systems for control of antibiotic contamination in milk and eggs with the limit of detection of 10 mkg/kg and in meat with the limit of 20 mkg/kg.

Keywords: streptomycin, milk, eggs, meat, immunoassay.

 

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1I.I. Mechnikov Research and Development Institute for Vaccines and Sera, RAMS, Moscow 105064, Russia,
e-mail: instmech@iitp.ru;
2All-Russia Research and Development Institute of Veterinary Sanitation, Hygiene and Ecologyl, RAAS, Moscow 123022, Russia,
e-mail: kononenkogp@mail.ru

Received March 3, 2012

 

 

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