Сельскохозяйственная биология, 2011, № 6, с. 75-79

УДК 638.1:636.082:575.174.015.3

MICROSATELLITES AS A TOOL FOR EVALUATION OF ALLELE POOL DYNAMICS WHEN CREATION OF PRIOKSKY TYPE OF MIDDLE RUSSIAN HONEY BEE

N.A. Zinovieva¹, N.I. Krivtsov², M.S. Fornara^{1, 3}, E.A. Gladyr’^{1, 3}, A.V. Borodachev², S.A. Berezin², V.I. Lebedev²

The informative capacity of the test system for the analysis of seven microsatellites (A024, A88, A113, AP043, HB-C16-05, HB-THE-03, HB-C16-01) for the allele pool characteristics of Middle Russian (n = 65), Gray Mountain Caucasian (n = 70) and Prioksky type of Middle Russian (n = 88) honey bee breeds was studied. The average number of alleles, the number of informative alleles and the number of effective alleles per loci were 7.48±1.02, 3.62±0.71 and 3.38±0.56, respectively. The increase of allele pool genetic diversity in Prioksky type comparing to Middle Russian and Grey Mountain Caucasian breeds used in type breeding was observed: 9.57±1.88 allele per loci as against 6.86±1.55 and 6.00±1.84, respectively. The allele introduction of initial breeds was evaluated. It was shown that 7.9 per cent of common microsatellite diversity was due to intra population differences.

Keywords: genetic markers, microsatellites, heterogeneity, honey bee breeds.

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