УДК 637:637.068:57.088.3

MULTIPLEX DNA IDENTIFICATION OF SPECIES ORIGIN OF MEAT PRODUCTS

E.N. Konovalova, E.A. Gladyr’, N.A. Zinovieva

The test-system based on multiplex PCR applied for simultaneous identification of pig (Sus scrofa), cow (Bos taurus) and chicken (Gallus gallus) DNA in milled muscle tissue samples was developed. The high sensitivity and specificity of species origin determination of components in structure of mixed samples using developed method was shown (the detection efficiency of species specific DNA in animal samples of the relevant species and in mixed samples was similar). The application of developed test-system for origin identification of different food products including heat treated products (cow milk, cottage cheese, boiled beef, beef liver paste, beef and chicken bouillon, raw pork, beef and pork minced meat mixture, baked and bloated chicken, cheese and sausage) was shown.

Keywords: species origin identification, mitochondrial DNA, multiplex PCR, meat products.

ГНУ Всероссийский НИИ животноводства
Россельхозакадемии,

142132 Московская обл., Подольский р-н, пос. Дубровицы,
e-mail: konoval-elena@yandex.ru

Поступила в редакцию
3 октября 2011 года

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