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Konkova E.А., Lyascheva S.V., Zeleneva Yu.V., Kovalenko N.М. Characteristics of perspective wheat cultivars, admitted to cultivation in the lower Volga region of the Russian Federation, based on resistance to perynophorosis and tan spot pathogens. Sel'skokhozyaistvennaya Biologiya [Agricultural Biology], 2023, Vol. 58, № 5, p. 852-863.
(how to cite this article)

doi: 10.15389/agrobiology.2023.5.852eng

UDC: 633.11:632.4:577.2

Acknowledgements:
The authors thank the reviewers for their contribution to the peer review of this work.
Supported financially by the Russian Science Foundation, Project No. 19-76-30005

 

CHARACTERISTICS OF PERSPECTIVE WHEAT CULTIVARS, ADMITTED TO CULTIVATION IN THE LOWER VOLGA REGION OF THE RUSSIAN FEDERATION, BASED ON RESISTANCE TO PERYNOPHOROSIS AND TAN SPOT PATHOGENS

E.А. Konkova1 ✉, S.V. Lyascheva 1, Yu.V. Zeleneva2, N.М. Kovalenko2

1Federal Center of Agriculture Research of the South-East Region, 7, ul. Tulaikova, Saratov, 410010 Russia, e-mail baukenowaea@mail.ru (✉ corresponding author), lyaschevasveta@yandex.ru;
2All-Russian Research Institute of Plant Protection, 3, sh. Podbel’skogo, St. Petersburg, 196608 Russia, e-mail zelenewa@mail.ru, nadyakov@mail.ru

ORCID:
Konkova E.A. orcid.org/0000-0001-8607-2301
Zeleneva Yu.V. orcid.org/0000-0001-9716-288X
Lyashcheva S.V. orcid.org/0000-0002-6790-0770
Kovalenko N.M. orcid.org/0000-0001-9577-8816

Final revision received August 07, 2023
Accepted October 03, 2023

Annual monitoring of the phytosanitary condition of wheat crops in the Saratov region evidences the active development of leaf spots and the accumulation of their infectious potential. Pyrenophora tritici-repentis is the causative agent of pyrenophora, or tan spot, and one of the dangerous wheat diseases. Bipolaris sorokiniana is the causative agent of spot blotch, a potentially dangerous wheat disease. In this work, as a result of field and laboratory assessment of 20 varieties of bread wheat (Triticum aestivum L.) cultivated in the Lower Volga region, samples resistant to the pathogen of tan spot, as well as moderately resistant to the pathogen of spot blotch, were identified for the first time. The results of identification of the Tsn1/tsn1 gene allele using the molecular marker Xfcp623 showed that among the studied wheat samples, the Tsn1 gene was predominant in winter wheat varieties. For the first time, statistically significant differences were revealed between the average values of field phytopathological estimates of resistance to P. tritici-repentis among varieties from different resistance groups (RR, R, MS and S). The purpose of the study is to assess the resistance of winter and spring bread wheat varieties (Triticum aestivum L.) cultivated in the Lower Volga region to tan spot and spot blotch pathogens and to identify the dominant/recessive allele of the Tsn1 gene in plants. The work examined 13 varieties of winter bread wheat (Gostianum 237, Lutescens 230, Saratovskaya 8, Guberniya, Mironovskaya 808, Donskaya bezostaya, Saratovskaya 90, Zhemchuzhina Povolzhya, Saratovskaya 17, Kalach 60, Podruga, Anastasia, Sosedka) and 7 varieties of spring bread wheat (Favorit, Prokhorovka, Yugo-Vostochnaya 2, Saratovskaya 70, Saratovskaya 73, Belyanka, Lebedushka). Of these, 16 varieties were approved for use in 2022. Sosedka is a promising variety of winter bread wheat; Gostianum 237, Lutescens 230, Saratovskaya 8 are the Saratov selection varieties of winter bread wheat produced in the first half of the 20th century. Field trials were carried out in 2020-2022 in the breeding nursery of the Federal Center of Agriculture Research of the South- East Region (Saratov). The response of wheat varieties to natural infection by a local population of the yellow spot pathogen was assessed under field conditions using a modified and extended Saari-Prescott scale. In lab tests, plant leaves were infected with P. tritici-repentis and B. sorokiniana. The inoculum consisted of a mixture of several fungal isolates from plant infectious material 2022 from the Saratov (P. tritici-repentis, ToxA), and Leningrad (B. sorokiniana) regions and the Republic of Kazakhstan (P. tritici-repentis, ToxB) obtained from the collection of the All-Russian Research Institute of Plant Protection. For phytopathological assessment of P. tritici-repentis, a scoring scale was used that characterizes the development degree of necrosis and chlorosis. When assessing the resistance of wheat to spot blotch caused by B. sorokiniana, we used a scale developed at the All-Russian Research Institute of Plant Protection. Genomic DNA was isolated from leaves of 5-day-old wheat seedlings using the standard CTAB/chloroform method. After quantification, the DNA concentration was normalized to 30 ng/μl for subsequent PCR. Screening for the presence of a dominant or recessive gene allele (Tsn1 or tsn1) in the varieties was carried out using PCR with primer pairs Xfcp623F/Xfcp623R (ZAO Evrogen, Russia). The proportion of varieties resistant to P. tritici-repentis isolates capable of producing toxins encoded by the ToxA and Tox B genes was 40 %, to the isolate with ToxA — 55 %, with Tox B — 60 %. The proportion of varieties with moderate resistance to Bipolaris sorokiniana was only 15 %. The genotypes of three winter bread wheat varieties (Gostianum 237, Mironovskaya 808, Podruga) and 5 genotypes of spring bread wheat (Favorit, Prokhorovka, Saratovskaya 70, Saratovskaya 73, Belyanka) which turned out to be resistant to tan spot in laboratory and three-year field trials are of the greatest interest. Molecular screening of these varieties also confirmed resistance to the PtrToxA producer, since they had tsn1, which indicates their resistance to the fungal toxin protein PtrToxA. These varieties should be involved into breeding programs in the region to improve the resistance of wheat to brown spot and blight pathogens. 

Keywords: wheat, Pyrenophora tritici-repentis, Bipolaris sorokiniana, ToxA, Tsn1, PCR, yellow leaf spot, tan spot, spot blotch.

 

REFERENCES

  1. Kovalenko N.M., Shaydayuk E.L., Gul’tyaeva E.I. Biotekhnologiya i selektsiya rasteniy, 2022, 5(2): 15-24 CrossRef (in Russ.).
  2. Bankina B., Bimšteine G., Arhipova I., Kaneps J., Darguža M. Impact of crop rotation and soil tillage on the severity of winter wheat leaf blotches. Rural Sustainability Research, 2021, 45(340): 21-27 CrossRef
  3. Effertz R.J., Meinhardt S.W., Anderson J., Jordahl J.G., Francl L.J. Identification of a chlorosis-inducing toxin from Pyrenophora tritici-repentis and the chromosomal location of an insensitivity locus in wheat. Phytopathology, 2002, 92(5): 527-533 CrossRef
  4. Ramos E.R., Kutcher R., Dallagnol J. Pyrenophora tritici-repentis: a worldwide threat to wheat. In: Wheat [Working Title]. IntechOpen, 2023 CrossRef
  5. Corsi B., Percival-Alwyn L., Downie R.C., Venturini L., Iagallo E.M., Mantello C.C., McCormick-Barnes Ch., Theen See P., Oliver R.P., Moffat C.S., Cockram J. Genetic analysis of wheat sensitivity to the ToxB fungal effector from Pyrenophora tritici-repentis, the causal agent of tan spot. Theoretical and Applied Genetics, 2020, 133: 935-950 CrossRef
  6. Strelkov S., Lamari L. Host parasite interactions in tan spot [Pyrenophora tritici-repentis] of wheat. Canadian Journal of Plant Pathology, 2003, 25(4): 339-349 CrossRef
  7. Faris J.D., Overlander M.E., Kariyawasam G.K., Carter A., Xu S.S., Liu Z. Identification of a major dominant gene for race-nonspecific tan spot resistance in wild emmer wheat. Theoretical and Applied Genetics, 2020, 133(3): 829-841 CrossRef
  8. Gourlie R., McDonald M., Hafez M., Ortega-Polo R., Low K.E., Abbott D.W., Strelkov S. E., Daayf F., Aboukhaddou R. The pangenome of the wheat pathogen Pyrenophora tritici-repentis reveals novel transposons associated with necrotrophic effectors ToxA and ToxB. BMC Biology, 2022, 20(1): 239 CrossRef
  9. Friesen T.L., Holmes D.J., Bowden R.L., Faris J.D. ToxA is present in the u.s. Bipolaris sorokiniana population and is a significant virulence factor on wheat harboring Tsn1. Plant Disease. 2018, 102(12): 2446-2452 CrossRef
  10. Bertagnolli V.V., Ferreira J.R., Liu Z., Rosa A.C., Deuner C.C. Phenotypical and genotypical characterization of Pyrenophora tritici-repentis races in Brazil. European Journal of Plant Pathology, 2019, 154(4): 995-1007 CrossRef
  11. Askhadullin Dan.F., Askhadullin Dam.F., Vasilova N.Z., Zharekhina T.V., Gayfullina G.R., Khusainova I.I., Bagavieva E.Z., Tazutdinova M.R. Zashchita i karantin rasteniy, 2018, 9: 17-19 (in Russ.).
  12. Novakazi F., Afanasenko O., Lashina N., Platz G. J., Snowdon R., Loskutov I., Ordon F. Genome-wide association studies in a barley (Hordeum vulgare) diversity set reveal a limited number of loci for resistance to spot blotch (Bipolaris sorokiniana). Plant Breeding, 2020, 139(3): 521-535 CrossRef
  13. Gupta P.K., Chand R., Vasistha N.K., Pandey S.P., Kumar U., Mishra V.K., Joshi A.K. Spot blotch disease of wheat: the current status of research on genetics and breeding. Plant Pathology, 2018, 67(3): 508-531 CrossRef
  14. Sidorov A.V., Zakharov V.G., Tyryshkin L.G. Izvestiya Sankt-Peterburgskogo gosudarstvennogo agrarnogo universiteta, 2018, 53: 76-79 CrossRef (in Russ.).
  15. Kremneva O.Yu., Kostenko I.A., Pachkin A.A., Danilov R.Yu., Ponomarev A.V., Kim Yu.S. Zernovoe khozyaystvo Rossii, 2020, 3(69): 61-66 CrossRef (in Russ.).
  16. Makarova M.A., Karacheva G.S., Lomakina I.V. Zashchita i karantin rasteniy, 2018, 8: 41-43 (in Russ.).
  17. Akulov A.Yu. Biologicheskie osobennosti Bipolaris sorokiniana (Sacc. in Sorokin) Shoem i differentsirovannaya diagnostika vozbuditeley kornevoy gnili i chernogo zarodysha yachmenya. Kandidatskaya dissertatsiya [Biological properties of Bipolaris sorokiniana (Sacc. in Sorokin) Shoem and differentiated diagnosis of pathogens of root rot and black germ of barley. PhD Thesis]. Khar’kov, 2006: 232 (in Russ.).
  18. Lillemo M., Joshi A.K., Prasad R., Chand R., Singh R. QTL for spot blotch resistance in bread wheat line Saar co'locate to the biotrophic disease resistance loci Lr34 and Lr46. Theoretical and Applied Genetics, 2013, 126(3): 711-719  CrossRef
  19. Joshil A.K., Chand R. Variation and inheritance of leaf angle, and its association with spot blotch (Bipolaris sorokiniana) severity in wheat (Triticum aestivum). Euphytica, 2002, 124: 283-291 CrossRef
  20. Mironenko N.V., Kovalenko N.M. Vestnik zashchity rasteniy, 2018, 2(96): 12-16 (in Russ.).
  21. Kon’kova E.A., Lyashcheva S.V. Zernovoe khozyaystvo Rossii, 2020, 3(69): 67-71 CrossRef (in Russ.).
  22. Kumarbayeva M., Kokhmetova A., Kovalenko N.M., Atishova M., Keishilov Z., Aitymbetova K. Characterization of Pyrenophora tritici-repentis (tan spot of wheat) races in Kazakhstan. Phytopathologia Mediterranea, 2022, 61(2): 243-257 CrossRef
  23. Gosudarstvennyy reestr selektsionnykh dostizheniy, dopushchennykh k ispol’zovaniyu. Tom 1. «Sorta rasteniy» (ofitsial’noe izdanie) [State register of selection achievements approved for use. Volume 1. Plant varieties (official publication)]. Moscow, 2022: 646 (in Russ.).
  24. Kolomiets T.M., Pakholkova E.V., Dubovaya L.P. Otbor iskhodnogo materiala dlya sozdaniya sortov pshenitsy s dlitel’noy ustoychivost’yu k septoriozu [Selection of parental material for breeding wheat varieties with long-term resistance to septoria]. Moscow, 2017: 56 (in Russ.).
  25. Mikhaylova L.A., Mironenko N.V., Kovalenko N.M. Zheltaya pyatnistost’ pshenitsy. Metodicheskie ukazaniya po izucheniyu vozbuditelya zheltoy pyatnistosti Pyrenophora tritici-repentis i ustoychivosti sortov [Yellow spot of wheat. Guidelines for studying the causative agent of yellow spot Pyrenophora tritici-repentis and resistance of varietie]. St. Petersburg, 2012: 64 (in Russ.).
  26. Smurova S.G. Novye istochniki i donory ustoychivosti pshenitsy k Cochliobolus sativus Drechs. ex Dastur. Kandidatskaya dissertatsiya [New donors of wheat resistance to Cochliobolus sativus Drechs. ex Dastu. PhD Thesis]. St. Petersburg, 2008: 236 (in Russ.).
  27. Doyle J.J., Doyle J.L. Isolation of plant DNA from fresh tissue. Focus, 1990, 12(1): 13-15.
  28. Röder M.S., Korzun V., Wendehake K., Plaschke J., Tixier M.H., Leroy Ph., Ganal M.W. A microsatellite map of wheat. Genetics, 1998, 149(4): 2007-2023 CrossRef
  29. Zhang Z., Friesen T.L., Simons K.J., Xu S.S., Faris J.D. Development, identification, and validation of markers for marker-assisted selection against the Stagonospora nodorum toxin sensitivity genes Tsn1 and Snn2 in wheat. Molecular Breeding, 2009, 23: 35-49 CrossRef
  30. Faris J.D., Zhang Z., Lu H.J., Lu S.W., Reddy L., Cloutier S., Fellers J.P., Meinhardt S.W., Rasmussen J.B., Xu S.S., Oliver R.P., Simons K.J., Friesen T.L. A unique wheat disease resistance-like gene governs effector-triggered susceptibility to necrotrophic pathogens. Proceedings of the National Academy of Sciences USA, 2010, 107(30): 13544-13549 CrossRef
  31. Trukhacheva N.V. Meditsinskaya statistika: uchebnoe posobie [Medical statistics: textbook](in Russ.). Rostov-na-Donu, 2017 (in Russ.).
  32. Kokhmetova A.M., Ali S., Sapakhova Z., Atishova M.N. Vavilovskiy zhurnal genetiki i selektsii, 2018, 22(8): 978-986 CrossRef (in Russ.).
  33. Mironenko N.V., Baranova O.A., Kovalenko N.M., Afanasenko O.S., Mikhaylova L.A. Vestnikzashchity rasteniy, 2017, 3 (93): 23-27 (in Russ.).
  34. Mironenko N.V., Baranova O.A., Kovalenko N.M. Mikologiya i fitopatologiya, 2019, 53(2): 115-123 CrossRef (in Russ.).

 

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