Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2017, Vol. 52, № 5, p. 964-975
(how to cite this article).

doi: 10.15389/agrobiology.2017.5.964eng

UDC 633.491:631.527.51:577.21

Acknowlegdgements:
Studies of plant resistance to late blight in the epiphytotic 2016, pollen fertility and molecular screening in 2016-2017 (VIR, St. Petersburg, Russia) were supported by Russian Science Foundation (grant № 16-16-04125). Hybridization and assessment of plant resistance to late blight and Potato virus Y were carried out in 2012-2015 (Swedish University of Agricultural Sciences — SLU, Sweden) with the financial support from the E. & I. Nilssons Foundation

 

FACILITATION OF INTROGRESSIVE HYBRIDIZATION OF WILD
POLYPLOID MEXICAN POTATO SPECIES USING DNA MARKERS
OF R GENES AND OF DIFFERENT CYTOPLASMIC TYPES

N.M. Zoteyeva1, O.Yu. Antonova1, N.S. Klimenko1, O.V. Apalikova1,
U. Carlson-Nilsson1, 3, Yu.I. Karabitsina1, Yu.V. Ukhatova1,
T.A. Gavrilenko1, 4

1Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources, Federal Agency of Scientific Organizations, 42-44, ul. Bol’shaya Morskaya, St. Petersburg, 190000 Russia,
e-mail tatjana9972@yandex.ru (corresponding author);
2The Nordic Genetic Resource Centre (NordGen), P.O. Box 41, SE-230 53 Alnarp, Sweden;
3Swedish University of Agricultural Sciences (SLU), P.O. Box 7070, SE-750 07 Uppsala, Sweden;
4Saint-Petersburg State University, Biological Department, 7/9, Universitetskaya nab., St. Petersburg, 199034 Russia

ORCID:
Zoteyeva N.M. orcid.org/0000-0003-2266-0467
Antonova O.Yu. orcid.org/0000-0001-8334-8069
Klimenko N.S. orcid.org/0000-0002-6213-4421
Apalikova O.V. orcid.org/0000-0002-1188-2446
Carlson-Nilsson U. orcid.org/0000-0002-6467-2215
Karabitsina Yu.I. orcid.org/0000-0002-8384-5134
Ukhatova Yu.V. orcid.org/0000-0001-9366-0216
Gavrilenko T.A. orcid.org/0000-0002-2605-6569

Received June 27, 2017

 

Nowadays potato breeding is targeting to develop genetically divers high yielding varieties with multiple pathogen resistance traits. Interspecific hybridization jointed with marker-assistant-selection (MAS) can effectively combine the R genes from different resistance sources. Additionally to effective pyramiding the target genes, MAS allows to restrict introgression of genetic factors conferring the undesirable traits, for example, male sterility of interspecific hybrids associated with Solanum stoloniferum-derived W/gamma cytoplasm that complicate the traditional breeding. Current study is targeting to search for the opportunities to improve the efficiency of introgressive hybridization between common potato and Mexican polyploid species Solanum. neoantipoviczii (= S. stoloniferum) and S. guerreroense using MAS with DNA markers for different cytoplasmic types and markers associated with major R-genes to the most harmful potato pathogens. DNA-based markers of genes for late blight resistance (R2 like, R3a, Rpi-blb1, Rpi-sto1), for extreme resistance to Potato virus Y (PVY) (Ryadg, Rysto, Ry-fsto) and for H1 gene for resistance to the root cyst nematode (Globodera rostochiensis, pathotype Ro1) were used in this study. Based on the MAS, hybrid genotypes with different combinations of these markers were selected. Among them, there were the clones with high field resistance to late blight and to PVY. Of 29 hybrid clones from different combinations of crossing with polyploid Mexican species used as the maternal forms, 15 had a W/g cytoplasmic type and were male sterile; both these traits were maternally inherited. The remaining hybrids with W/a cytoplasm produced fertile pollen and were used in interspecific crosses as pollinators. Selection of resistant clones with W/alpha cytoplasm and elimination of genotypes with sterile W/g cytoplasm among wild species germplasm could increase the probability of obtaining male fertile introgressive lines. This approach allows to obtain the multi-species hybrid genotypes that combine R genes for resistance to pathogens from different Mexican species and to avoid various types of male sterility in breeding. The joint use of two systems of DNA markers, i.e. nuclear markers associated to R genes, and cytoplasmic markers for male sterility factors, could reduce costs and increase efficiency of target gene pyramiding programs.

Keywords: Solanum spp., potato, DNA markers, R genes, cytoplasmic types, interspecific hybridization.

 

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