doi: 10.15389/agrobiology.2016.5.731eng

UDC 635.64:632.937

The authors thank Prof. E.I. Savel’eva (Research Institute of Hygiene, Occupational Pathology and Ecology, the Federal Medical and Biological Agency) for carrying out chemical analysis of volatile compounds.



E.A. Stepanycheva, M.O. Petrova, A.V. Shchenikova, T.D. Chermenskaya

All-Russian Research Institute of Plant Protection, Federal Agency of Scientific Organizations,3, sh. Podbel’skogo, St. Petersburg, 196608 Russia,

Received February 19, 2016


Until recently, induced resistance to pathogens and phytophages considered separately and only in recent years the attention are being paid to the possibility of an induced cross-resistance. The aim of this work was to study the nature of the chemical interaction between plants and phytopathogenic microorganisms and arthropods phytophages, inhabiting the same ecological niche. The possibility of mutually-modifying effects of phytophagous and pathogens on quantitative and qualitative indicators of the defense response of tomato plants were shown. As the first order consumers the Western flower thrips Frankliniella occidentalis and whitefly Trialeurodes vaporariorum were chosen as most dangerous herbivores of greenhouse crops, and Pseudomonas syringae pv. tomato, a bacterial pathogen of tomato mottle, was used. The evaluation criteria were the changes in behavioral responses of herbivores and their demographic parameteres, and for pathogen the degree of infection development was expressed in points. Under the insects’ free choice, the tomato plants previously infected with P. syringae, were more preferable by thrips, while remained not more attractive for whitefly. Attraction of thrips to infected plants may be due to the appearance and increasing content of volatile substances such as 2-methylbutanoic acid and dodecane, which are components of the thrips pheromone and allomons. Under the primary damage of plants by thrips and whitefly the further pathogen development differed (e.g., the thrips suppressed the disease, while the whitefly served as promoters for its development). Inhibition of the pathogen on plants damaged by thrips, may be due to an increase in the content of these chemical compounds, such as, for example, (E)-β-ocimene and a-humulene that are part of many essential oils and plant extracts with antimicrobial activity. The content of these same substances was increased in plants in response to inoculation with the pathogen. The results indicate both the differences and similarities of some signaling pathways and mechanisms of defense reaction in plants in response to induction phytophagous or phytopathogens. It identified the induced resistance and partial antagonism (down to completely opposite effect — the reduction of plant resistance) with respect to a group of consumers. A thorough assessment of the nature of these responses, its biochemical and molecular genetic basis will contribute to the strategy of environment-friendly plant protection.

Keywords: western flower thrips, greenhouse whitefly, induced defense, Pseudomonas syringae.


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