doi: 10.15389/agrobiology.2016.5.731eng

UDC 635.64:632.937

The authors thank Prof. E.I. Savel’eva (Research Institute of Hygiene, Occupational Pathology and Ecology, the Federal Medical and Biological Agency) for carrying out chemical analysis of volatile compounds.

 

ALLELOCHEMICS: AN INTERACTION BETWEEN PHYTOPHAGЕS AND Pseudomonas syringae pv. tomato ON TOMATO Solanum lycopersicum PLANTS

E.A. Stepanycheva, M.O. Petrova, A.V. Shchenikova, T.D. Chermenskaya

All-Russian Research Institute of Plant Protection, Federal Agency of Scientific Organizations,3, sh. Podbel’skogo, St. Petersburg, 196608 Russia,
e-mail stepanycheva@yandex.ru, tchermenskaya@yandex.ru

Received February 19, 2016

 

Until recently, induced resistance to pathogens and phytophages considered separately and only in recent years the attention are being paid to the possibility of an induced cross-resistance. The aim of this work was to study the nature of the chemical interaction between plants and phytopathogenic microorganisms and arthropods phytophages, inhabiting the same ecological niche. The possibility of mutually-modifying effects of phytophagous and pathogens on quantitative and qualitative indicators of the defense response of tomato plants were shown. As the first order consumers the Western flower thrips Frankliniella occidentalis and whitefly Trialeurodes vaporariorum were chosen as most dangerous herbivores of greenhouse crops, and Pseudomonas syringae pv. tomato, a bacterial pathogen of tomato mottle, was used. The evaluation criteria were the changes in behavioral responses of herbivores and their demographic parameteres, and for pathogen the degree of infection development was expressed in points. Under the insects’ free choice, the tomato plants previously infected with P. syringae, were more preferable by thrips, while remained not more attractive for whitefly. Attraction of thrips to infected plants may be due to the appearance and increasing content of volatile substances such as 2-methylbutanoic acid and dodecane, which are components of the thrips pheromone and allomons. Under the primary damage of plants by thrips and whitefly the further pathogen development differed (e.g., the thrips suppressed the disease, while the whitefly served as promoters for its development). Inhibition of the pathogen on plants damaged by thrips, may be due to an increase in the content of these chemical compounds, such as, for example, (E)-β-ocimene and a-humulene that are part of many essential oils and plant extracts with antimicrobial activity. The content of these same substances was increased in plants in response to inoculation with the pathogen. The results indicate both the differences and similarities of some signaling pathways and mechanisms of defense reaction in plants in response to induction phytophagous or phytopathogens. It identified the induced resistance and partial antagonism (down to completely opposite effect — the reduction of plant resistance) with respect to a group of consumers. A thorough assessment of the nature of these responses, its biochemical and molecular genetic basis will contribute to the strategy of environment-friendly plant protection.

Keywords: western flower thrips, greenhouse whitefly, induced defense, Pseudomonas syringae.

 

Full article (Rus)

Full text (Eng)

 

REFERENCES 

  1. Duijff B.J., Pouhair D., Olivain C., Alabouvette C., Lemanceau P. Implication of systemic induced resistance in the suppression of Fusarium wilt of tomato by Pseudomonas fluorescens WCS417r and by nonpathogenic Fusarium oxysporum Fo47. Eur. J. Plant Pathology, 1998, 104: 903-910.
  2. Ran L.X., Li Z.N., Wu G.J., van Loon L.C., Bakker P.A.H.M. Induction of systemic resistance against bacterial wilt in Eucalyptus urophylla by fluorescent Pseudomonas spp. Eur. J. Plant Pathology, 2005, 113: 59-70 CrossRef
  3. Burov V.N., Petrova M.O., Selitskaya O.G., Stepanycheva E.A., CHer-
    menskaya T.D., Shamshev I.V. Indutsirovannaya ustoichivost' rastenii k fitofagam. [Induced plant resistance to phytophags]. Moscow, 2012 (in Russ.).
  4. Felton G.W., Summers C.B., Mueller A.J. Oxidative responses in soybean foliage to herbivory by bean leaf beetle and three cornered alfalfa hopper. J. Chem. Ecol., 1994, 20(3): 639-650 CrossRef
  5. Srinivas P., Danielson S.D., Smith C.M., Foster J.E. Cross-resistance and resistance longevity as induced by bean leaf beetle, Cerotoma trifurcate and soybean looper, Pseudoplusia includens herbivory on soybean. J. Insect Sci., 2001, 1: 5.
  6. Stepanycheva E.A., Chermenskaya T.D., Petrova M.O., Shchenikova A.V., Burov V.N., Savel'eva E.I. Evraziatskii entomologicheskii zhurnal, 2007, 6(1): 19-24 (in Russ.).
  7. De Moraes C.M., Mescher M.C., Tumlinson J.H. Caterpillar-induced nocturnal plant volatiles repel conspecific females. Nature, 2001, 410: 577-580 CrossRef 
  8. Delphia C.M., Mescher M.C., De Moraes C.M. Induction of plant volatiles by herbivores with different feeding habits and the effects of induced defenses on host-plant selection by thrips. J. Chem. Ecol., 2007, 33(5): 997-1012 CrossRef
  9. Yalpani N., Raskin I. Salicylic acid: a systemic signal in induced plant disease resistance. Trends Microbiol., 1993, 1(3): 88-92 CrossRef
  10. Sudhakar N., Nagendra-Prasad D., Mohan N., Murugesan K. Induction of systemic resistance in Lycopersicon esculentum cv. PKM1 (tomato) against Cucumber mosaic virus by using ozone. J. Virol. Methods, 2007, 139(1): 71-77 CrossRef
  11. Poliksenova V.D. Vestnik Belorusskogo GU, 2009, 1: 48-60 (in Russ.).
  12. Tyuterev S.L. Mekhanizmy deistviya fungitsidov na fitopatogennye griby [Action of fungicides on fungal phytopathogens]. St. Petersburg, 2010 (in Russ.).
  13. Moran P.Plant-mediated interactions between insects and a fungal plant pathogen and the role of plant chemical responses to infections. Oecologia, 1998, 115(4): 523-530 CrossRef
  14. HatcherP.E., Paul N.D. Beetle grazing reduces natural infection of Rumex obtusifolius by fungal pathogens. New Phytologist, 2000, 146: 325-333 CrossRef
  15. Rostas M., Hilker M. Asymmetric plant-mediated cross-effects between a herbivorous insect and a phytopathogenic fungus. Agr. Forest Entomol., 2002, 4(3): 223-231CrossRef
  16. Thaler J.S., Agrawal A.A., Halitschker R. Salicylate-mediated interactions between pathogens and herbivores. Ecology, 2010, 91(4): 1075-1082 CrossRef
  17. Russo V.M., Russo B.M., Peters M., Perkins-Veazie P., Cartwright B. Interaction of Colletotrichum orbiculare with thrips and aphid feeding on watermelon seedlings. Crop Protection, 1997, 16: 581-584 CrossRef
  18. De Vos M., Van Zaanen W.V., Koornneef A., Korzelius J.P., Dicke M., Van Loon L.C., Pieterse C.M.J. Herbivore-induced resistance against microbial pathogens in Arabidopsis. Plant Physiol., 2006, 142(1): 352-363 CrossRef
  19. Stout M.J., Fidantsef A.L., Duffey S.S., Bostock R.M. Signal interactions in pathogen and insect attack: systemic plant-mediated interactions between pathogens and herbivores of the tomato, Lycopersicon esculentum. Physiol. Mol. Plant P., 1999, 54(3-4): 115-130. CrossRef
  20. Edris A.E., Farrag E.S. Antifungal activity of peppermint and sweet basil essential oils and their major aroma constituents on some plant pathogenic fungi from the vapor phase. Nahrung/Food, 2003, 47(2): 117-121 CrossRef
  21. Pitarokili D., Tzakou O., Loukis A., Harvala C. Volatile metabolites from Salvia fruticosa as antifungal agents in soilborne pathogens.J. Agric. Food Chem., 2003, 51: 3294-3301 CrossRef
  22. Chorianopoulos N., Kalpoutzakis E., Aligiannis N., Mitaku S., Nychas G.-J., Haroutounian S.A. Essential oils of Satureja, Origanum, and Thymus species: chemical composition and antibacterial activities against foodborne pathogens. J. Agric. Food Chem.,2004, 52: 8261-8267 CrossRef
  23. Bridges J.R. Effects of terpenoid compounds on growth of symbiotic fungi associated with the southern pine beetle. Phytopathology, 1987, 77: 83-85 CrossRef
  24. Glisik S.B., Milojevic S.Z., Dimitrijevic S.I., Orlovic A.M., Skala D.U. Antimicrobial activity of the essential oil and different fractions of Juniperus communis L. and a comparison with some commercial antibiotics. J. Serb. Chem. Soc., 2007, 72(4): 311-320 CrossRef
  25. Kohzaki K., Gomi K., Yamasaki-Kokudo Y., Ozawa R., Takabayashi J., Akimitsu K. Characterization of a sabinene synthase gene from rough lemon (Citrus jambhiri). J. Plant Physiol., 2009, 166(15): 1700-1704 CrossRef
  26. Islam M.T., Ahn S.-Y., Cho S.-M., Yun H.K. Isolation of antibacterial compounds from hairy vetch (Vicia villosa) against grapevine crown gall pathogen. Hortic. Environ. Biote., 2013, 54(4): 338-345 CrossRef
  27. Srinivasan G.V., Sharanappa P., Leela N.K., Sadashiva C.T., Vijay-
    an K.K. Chemical composition and antimicrobial activity of Leea indica (Burm. f.) Merr. flowers. Nat. Prod. Radiance, 2009, 8: 488-493.
  28. Camele I., Altieri L., De Martino L., De Feo V., Mancini E., Rana G.L. In vitro control of post-harvest fruit rots fungi by some plant essential oil components. Int. J. Mol. Sci., 2012, 13(2): 2290-2300 CrossRef
  29. Suzuki T., Haga K., Kataoka M., Tsutsumi T., Nakano Y., Matsuya-
    ma S., Kuwahara Y. Secretion of thrips VIII. Secretions of the two Ponticulothrips species (Thysanoptera: Phlaeothripidae). Appl. Entomol. Zool., 1995, 30: 509-519.
  30. Suzuki T., Haga K., Tsutsumi T., Matsuyama S. Analysis of anal secretions from Phlaeothripine thrips. J. Chem. Ecol., 2004, 30: 409-423 CrossRef
  31. Abdullah Z.S., Ficken K.J., Greenfield B.P.J., Butt T.M. Innate responses to putative ancestral hosts: Is the attraction of western flower thrips to pine pollen a result of relict olfactory receptors? J. Chem. Ecol., 2014, 40: 534-540 CrossRef
  32. Dublon I.A.N. The aggregation pheromone of the western flower thrips. Thesis: Doctor of Philosophy by research (Ph.D.). Keele. Staffordshire. UK, 2009.
  33. Terry I., Walter G.H., Moore C., Roemer R., Hull C. Odor-mediated push-pull pollination in Cycads. Science, 2007, 318: 70 CrossRef
  34. Leiss K.A., Maltese F., Choi Y.H., Verpoorte R., Klinkhamer P.G.L. Identifcation of chlorogenic acid as a resistance factor for thrips in Chrysanthemum. Plant Physiol., 2009, 150: 1567-1575 CrossRef
  35. Yang T., Stoopen G., Thoen M., Wiegers G., Jongsma M.A. Chrysanthemum expressing a linalool synthase gene ‘smells good’, but ‘tastes bad’ to western flower thrips. Plant Biotechnol. J., 2013, 11(7): 875-882 CrossRef
  36. Danilov D.A., Zykova I.D., Efremov A.A. Uspekhi sovremennogo estestvoznaniya, 2013, 9: 156-158 (in Russ.).

back