doi: 10.15389/agrobiology.2013.5.61eng
UDC 633.18:631.522/.524:575.222.7
STERILITY OF INTERSUBSPECIFIC HYBRIDS OF Oryza sativa L. IN CONNECTION WITH SEARCH OF GENES OF WIDE COMPATIBILITY AND CORRECT REFERENCE OF SAMPLES TO SUBSPECIES indica AND japonica
E.M. Kharitonov, Yu.K. Goncharova
All-Russian Research Institute of Rice, Russian Academy of Agricultural
Sciences,
pos. Belozerniy, Krasnodar, 350921 Russia,
e-mail: serggontchar@mail.ru
Received October 22, 2012
In rice (Oryza sativa L.) between indica and japonica subspecies a number of transitional forms both on phenotype and on physiological determinants present, which have the characteristic features in their development and adaptation to stress. The heterosis effect in intersubspecific hybrids (japonica/indica) can be of 100 % and more. The superhigh heterosis can be used during intersubspecific hybridization on conditions that the genes of wide compatibility (WC), which ensure a grain content more of 70 %, will be involve in breeding. The authors made the crossings between the samples of domestic and foreign breeding from japonica subspecies. The analysis of grain formation in the hybrid combinations did not allow to reveal WC genes in studied varieties of Russian selection defining high fertility of hybrids. The molecular scanning of hybrids and their parental forms was made with the use of 11 polymorphous SSR-markers, typical for subspecies. A possibility to obtain the fertile intersubspecific hybrids of rice and the list of trait used for differentiating samples to subspecies japonica and indica are discussed. The obtained results show that it is necessary to include additional characteristics into the list of trait for the taxonomic attributing samples to indica and japonica subspecies.
Keywords: rice, heterosis, hybrid, wide compatibility genes (WC), rice subspecies, japonica, indica.
REFERENCES
1. Ting M., Hai X., Quan X., Yan-hua G., Chunjie Z., Kai C., Jiayu W., Zhengjin X. Comparison of morphological and genetic differentiations in filial generation of cross between indica and japonica rice. Rice Sci., 2010, 17(1): 82-86.
2. Cheng K.S. Discrimination of indica-japonica subspecies in Asia сultivated rice. Kunming, Yunnan Science and Technology Press, 1993: 1-23.
3. Oka H.I. Origin of cultivated rice. Tokyo, Scientific Societies Press, 1988: 181-209.
4. Ting Y. The origin and evolution of cultivated rice in China. Acta Agron. Sin., 1957, 8: 243-260.
5. Chang T.T. The origin, evolution, cultivation, dissemination, and diversification of Asian and African rice. Euphytica, 1976, 25: 435-441.
6. Wang C.L., Zhang Z.L., Tang S.Z., Shi J.D. Exploitation of heterosis between indica and japonica by three-line method. I. Differentiation between indica-japonica sterility and cytoplasmic male sterility. Jiangsu J. Agr. Sci., 1992, 8: 1-7.
7. Zhang Q., Liu K.D., Yang G.P., Saghai M.A., Maroof S.A., Xu G., Zhou Z.Q. Molecular marker diversity and hybrid sterility in indica-japonica rice crosses. Theor. Appl. Genet., 1997, 95: 112-118.
8. Yang G.P., Maroof S.A., Xu C.G., Zhang Q., Biyashev R.M. Сomparative analysis of microsatellite DNA polymorphism in landraces and cultivars of rice. Mol. Gen. Genet., 1994, 245: 187-194.
9. Garris A.J., Tai T.H., Coburn J. et al. Genetic structure and diversity in Oryza sativa L. Genetics, 2005, 169: 1631-1638.
10. Mackill D.J. Classifying japonica cultivars with RAPD markers. Crop. Sci., 1995, 35: 889-894.
11. Gontcharova I.K., Gontcharov S.V. Hybrid rice breeding in Russia. Proc. 4th Intern. Symp. on hybrid rice «Hybrid rice for food security, poverty alleviation, and environmental protection» /S.S. Virmani, S.X. Mao, B. Hardy (eds.). Los Banos (Philippines), Int. Rice Res. Inst., 2003: 321-328.
12. Goncharova J.K. Fixing heterosis effect in rice hybrids by Strunnikov’s method. Proc. Int. Conf. on Integration of Science and Technology for Sustainable Development. Bangkok, Thailand, 2007: 393-396.
13. Zhang G.Q., Lu Y.G. Genetics of F1 pollen sterility in Oryza sativa. In: Rice genetics III. Manila, IRRI, 1996: 418-422.
14. Yuan L.P. Strategic hypotheses of breeding for hybrid rice (in Chinese). Hybrid Rice, 1987, 1: 1-3.
15. Ikehashi H., Araki H. Variety screening of compatibility types revealed in F1 fertility of distant cross in rice. Jpn. J. Breed., 1984, 34: 304-313.
16. Ikehashi H., Araki H. Genetics of F1 sterility in remote crosses of rice. In: Rice genetics: proceedings of the international rice genetics symposium, 27-31 May 1985. Manila, Philippines, IRRI, 1986: 119-130.
17. Ikehashi H., Wan J. Differentiation of alleles at seven loci for hybrid sterility in cultivated rice (Oryza sativa L.). In: Rice genetics: proceedings of the international rice genetics symposium, 16-20 October 1995. Manila, Philippines, IRRI, 1996: 404-408.
18. Xu Z.J., Chen W.F., Zhang B.L. Type difference and genetic study on the upmost internode vascular bundles in rice. Acta. Agron. Sin., 1996, 22(2): 167-172.
19. Taneichi T., Koide Y., Nishimoto D., Sano Y. Hybrid sterility gene S13 found in a distantly related rice species, O. longistaminata. Genes Genet. Syst., 2005, 80: 477.
20. Qiu S.Q., Kede L., Liang J., Song X., Xu C.G., Li X.H., Zhang Q. Delimitation of the rice wide compatibility gene S5n to a 40-kb DNA fragment. Theor. Appl. Genet., 2005, 111: 1080-1086.
21. Zhao Z.G., Jiang L., Zhang W.W., Yu C.Y., Zhu S.S., Xie K., Tian H., Liu L.L., Ikehashi H., Wan J.M. Fine mapping of S31, a gene responsible for hybrid embryo-sac abortion in rice (Oryza sativa L.). Planta, 2007, 226(5): 1087-1096.
22. Li D., Liangming C., Ling J. Fine mapping of S32(t), a new gene causing hybrid embryo sac sterility in a Chinese landrace rice (Oryza sativa L.). Theor. Appl. Genet., 2007, 114: 515-524.
23. Wang J., Liu K.D., Xu C.G., Li H.X., Zhang Q. The high level of wide compatibility of variety «Dular» has a complex genetic basis. Theor. Appl. Genet., 1998, 97: 407-412.
24. Collard B.C., Das A., Virk P.S., Mackill D.J. Evaluation of «quick and dirty» DNA extraction methods for marker-assisted selection in rice (Oryza sativa L.). Plant Breed., 2007, 126: 47-50.
25. Lyakhovkin A.G. Trudy po prikladnoi biologii. genetike i selektsii (Leningrad), 1987, 100: 63-74.
26. Lyakhovkin A.G. Trudy po prikladnoi biologii. genetike i selektsii (Leningrad), 1980, 69(1): 82-86.
27. Trubilin I.T., Shokov N.R., Kosenkov Yu.P. et al. Osnovnye morfologicheskie i aprobatsionnye proznaki sortov i gibridov zernovykh, zernobobovykh, krupyanykh i maslichnykh rastenii [Main Morphological and Approbation Features of Varieties and Hybrids of Cereals, Legumes, Cereals and Oilseeds]. Krasnodar, 2000.
28. Chen S.H., Mao C.Z., Zhan X.D., Si H.M., Sun Z.X. The construction of DH populations and RIL populations for indica-japonica and the differentiation of indica and japonica. Chinese J. Rice Sci., 2001, 15(4): 257-260.
29. Zhu Z.F., Sun C.Q., Fu Y.C., Zhang P.J., Wang X.K. Genetic diversities revealed by comparing Asia cultivated rice and common wild rice by SSR markers. Sci. Agric. Sin., 2002, 35(12): 1437-1441.
30. Wang Y., Qiu F., Hua Z., Dai G. Relationship of parental indica-japonica indexes with yield and grain quality traits of japonica hybrid rice in Northern China. Rice Sci., 2010, 17(3): 199-205.
31. Mao T., Xu H., Xu Q. et al. Comparison of morphological and genetic differentiations in filial generation of cross between indica and japonica rice. Rice Sci., 2010, 17(1): 82-86.
32. Morishima H., Oka H.I. Phylogenetic differentiation of cultivated rice, numerical evaluation of the indica-japonica differentiation. Jpn. J. Breed., 1981, 31: 402-413.
33. Ouyang Y.D., Chen J.J., Ding J.H., Zhang Q. Advances in the understanding of inter-subspecific hybrid sterility and wide-compatibility in rice. Chinese Sci. Bull., 2009, 54: 2332-2341.
