doi: 10.15389/agrobiology.2013.5.75eng
UDC 633/635:581.133.1:577.15
REDUCTION OF NITRATES BY ORGANS OF PLANTS IN FAMILIES OF Brassicaceae, Poaceae, Fabaceae
E.V. Boyarkin1, N.V. Dorofeev2, A.A. Peshkova2
1Irkutsk State Agricultural Academy,
12, pos. Molodezhniy, Irkutsk, 664038 Russia;
2Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of Russian Academy of Sciences,
132, ul. Lermontova, Irkutsk, 664033 Russia,
e-mail: dorofeev@sifibr.irk.ru
Received August 27, 2009
According to the ability to assimilate nitrate all plants are usually divided into three groups, including those with high nitrate reductase activity in plant root, low nitrate reductase activity in plant root and approximately equal nitrate reductase activity in root and leaves.The authors studied the assimilation of nitrates by different organs in plants of Brassicaceae (Raphanus sativus convar. oleifera, Raphanus sativus loba, Raphanus sativus, garden radish of the Zhara, Saksa, Ledyanaya sosul’ka varieties), Poaceae (spring wheat, winter wheat, barley, maize) and Fabaceae (Pisum sativum, Soja hispida, Lupinus perennis, garbanzo) in controlled conditions of development. The nitrate reductase activity, the content of nitrates in organs, and also the mass of dump matter were determined in 15-days plants. The most activity of nitrate reductase in all organs was revealed in Raphanus sativus convar. oleifera. This activity was highest in leaves almost in all studied species, which carry out the basic role in process of reduction of nitrates. So grouping the plants on their ability to reduce nitrates did not have clear boundaries. The species differ in this index even within the range of one family.
Keywords: nitrate reductase, nitrates, leaves, stems, roots, families.
REFERENCES
1. Brei S.M. Azotnyi obmen v rasteniyakh [Nitrogen Metabolism in Plants]. Moscow, 1986.
2. Izmailov S.F. Azotnyi obmen v rasteniyakh [Nitrogen Metabolism in Plants]. Moscow, 1986.
3. Polevoi V.V. Fiziologiya rastenii [Plant Physiology]. Moscow, 1989.
4. Dorofeev N.V., Peshkova A.A. Agrokhimiya, 2002, 9: 17-21.
5. Boyarkin E.V., Dorofeev N.V., Peshkova A.A. Fiziologiya i Biokhimiya Kul'turnykh Rastenii, 2004, 36(3): 257-261.
6. Fan X.H., Tang C., Rengel Z. Nitrate uptake, nitrate reductase distribution and their relation to proton release in five nodulated grain legumes. Ann. Bot., 2002, 90(3): 315-323.
7. Hoff T., Stummann B.M., Henningsen K.W. Structure, function and regulation of nitrate reductase in higher plants. Physiol. Plant, 1992, 84: 616-624.
8. Wallace W. Distribution of nitrate assimilation between the root and shoot of legumes and a comparison with wheat. Physiol. Plant, 1986, 66: 630-636.
9. Peshkova A.A. Fiziologiya i Biokhimiya Kul'turnykh Rastenii, 1990, 22(2): 142-147.
10. Peshkova A.A., Dorofeev N.V. Agrokhimiya, 1999, 12: 41-46.
11. Gojon A., Soussana J.F., Passama L., Robin P. Nitrate reduction in roots and shoots of barley (Hordeum vulgare L.) and corn (Zea mays L.) seedlings. I. 15N study. Plant Physiol., 1986, 82(1): 254-260.
12. Haba P., Aguera E., Benitez J., Maldonado J.M. Modulation of nitrate reductase activity in cucumber (Cucumis sativus ) roots. Plant Sci., 2001, 161(1): 231-237.
13. Caba M., Lluch C., Ligero F. Distribution of nitrate reductase activity in Vicia faba: Effect of nitrate and plant genotype. Physiol. Plant, 1995, 93(3): 667-672.
14. Gonzales E.M., Cadererizo P.M., Royuela M., Aparicio-Tejo P.M., Arre-
se-Igor N. Nitrate reduction in tendrils of semi-leafless pea. Physiol. Plant., 2001, 111(2): 329-335.
15. Аndrews M., Sutherland J.M., Tomas R.J.S., Sprent J.I. Dustribution of nitrate reductase activity in six legumes: the importance of the stem. New Phytol., 1984, 98(2): 301-310.
16. Sher K.N., Kumakov V.A., Chernov V.K. Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 1998, 5: 59-62.
