doi: 10.15389/agrobiology.2013.5.118eng

UDC 632.4:577.29:632.08

MOLECULAR ANALYSIS OF FUNGAL WOOD DECAY

E.E. Andronov1, E.P. Chizhevskaya1, A.G. Pinaev1, S.A. Pavlov2

1All-Russian Research Institute of Agricultural Microbiology, Russian Academy of Agricultural Sciences,
3, sh. Podbelskogo, St. Petersburg–Pushkin, 196608 Russia,
e-mail: eeandr@gmail.com;
2Peterhof State Museum Reserve,
1, ul. Razvodnaya, St. Petersburg, 198516 Russia,
e-mail: ciborium.8@gmail.com

Received June 28, 2012


A conventional analysis of fungal decay of wood is based on cultivation dependent approaches and can take up to several months. Here we demonstrate a possibility of molecular methods use for taxonomical and quantitative analyses of fungal wood decay. Using the sequencing of ITS libraries and real-time PCR we showed that 75 % of fungal population of the wood sample analysed is represented by Aureobasidium pullulans specie. The approach applied has great advantages over traditional cultivation dependent approaches because of its versatility, availability and cultivation independency. The method is characterized by its high speed (3-4 days) and low cost (1000-2000 rub. per sample). Our protocol’s main achievement is the ability to identify real diversity of fungal population. The method can be transferred in routine agricultural practice for fungal pathogen detection.    

Keywords: fungal decay of wood, ITS, Real-time PCR, Aureobasidium pullulans.

 

Full article (Rus)

 

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