UDC 634.10:631.52:575.222.73:581.143.6
METHODS OF TISSUES CULTURE AND POLYPLOIDY in vitro DURING BIGENERIC CROSSING OF PIP ORCHARD CROPS
R.V. Papikhin, S.A. Muratova
With the use of biotechnological methods the hybrid plants were obtained from seeds, which arised during bigeneric crossing of pip orchard crops. The results of investigations were presented regarding the induction of polyploidy in distant hybrids of pip orchard crops. The amitotic action of acenaphthene and colchicin on a development of plants was studied in the conditions in vitro. The analysis of morphological changes of sprouts and stomatal mechanism of plant leaves after the treatment by polyploidy chemicals was made. The determination of chromosomes number in root growing points of plants, obtained on nutrient media with 0.001 and 0.01 % acenaphthene and colchicin, confirms the chance of ploidy in hybrids.
Keywords: intergeneric hybridization, seed friut, tissue culture in vitro, polyploidy induction, amitotic, cytological analysis.
ГНУ Всероссийский НИИ генетики и селекции
плодовых растений им. И.В. Мичурина, |
Received September 25, 2008 |
