UDC 635.263:578.85/.86:578.2:577.217
THE DICER-LIKE PROTEINS CODING NUCLEOTIDE SEQUENCES IN SHALLOT Allium cepa L. var. aggregatum G. Don
A.V. Arkhipov, V.K. Vishnichenko
For the first time in the world literature the authors presented the evidence of expression in shallot plants(Allium cepa L. var. aggregatum G. Don) of nucleotide sequences coding the polypeptides homologous to known plant Dicer-like protein (DCL), obtained by PCR, sequencing and computer analysis. The investigated polypeptides contain specific for DCL-protein the dsRNA bind, PAZ and RIBOc domens. The solution of assigned task is available only with use of diagnostic oligonucleotide primers, the design of which was accomplished on the basis of information on EST-sequences (expressed sequence tags) in Allium sativum (GarlicESTdb integrated database). The results permit to draw a conclusion about high extent of divergence of DCL-proteins in Arabidopsis, poplar, rice, on the one hand, and the plants of Allium genus, on the other hand, that is revealed on level both nucleotide sequences of mRNA, and amino acid sequences of studied polypeptides.
Keywords: RNA-interference, Dicer&Dicer-like (DCL) proteins, shallot (Allium cepa L. var. aggregatum), Shallot virus X.
All-Russia Research and Development Institute of Agricultural Biotechnology, RAAS, Moscow 127550, Russia |
Received June 6, 2011 |
