УДК: 633.854.78:581.2:57.088.5
APPLICATION OF RAPD-PCR MARKERS FOR DIFFERENTIATION OF PHYSIOLOGICAL RACES IN Plasmopara halstedii (Farl.) Berl. et de Toni INFECTING SUNFLOWER IN KRASNODARSKII REGION
S.Z. Guchetl’, T.A. Chelyustnikova, M.V. Ivebor, T.S. Antonova, N.M. Araslanova, S.A. Ramazanova
By the method of DNA polymerase chain reaction (PCR) with 22 RAPD-primers (RAPD — random amplified polymorphic DNA) the authors analyzed the molecular-genetic poly-morphism in Plasmopara halstedii (Farl.) Berl. et de Toni, an agent of mildew of sunflower, for dif-ferentiation of five pathogen races isolated in Krasnodarskii region. It was revealed, that four races (330, 700, 710, 730) from five are similar genetically with sharply defined race 300.
Key words: downy mildew, sunflower, Plasmopara halstedii, races, PCR, RAPD, mark-ers, polymorphism, differentiation.
ГНУ Всероссийский НИИ масличных культур |
Поступила в редакцию |
