PLANT BIOLOGY
ANIMAL BIOLOGY
SUBSCRIPTION
E-SUBSCRIPTION
 
MAP
MAIN PAGE

 

 

 

 

doi: 10.15389/agrobiology.2019.4.713eng

UDC: 636.033:636.4:636.082.12:577.2

Acknowledgements:
The equipment of the Center for Biological Resources and Bioengineering of Farm Animals (Ernst Federal Science Center for Animal Husbandry)was used for the study.
Supported financially by the Ministry of Education and Science of the Russian Federation, a unique project number RFMEFI60417X0182

 

STUDY OF WUR10000125 POLYMORPHISM ASSOCIATION WITH MEAT, FATTENING AND REPRODUCTIVE TRAITS OF LANDRACE AND LARGE WHITE PIG BREEDS

O.V. Kostyunina, E.E. Melnikova, M.S. Fornara,
N.V. Bardukov, A.A. Sermyagin, G. Brem, N.A. Zinovieva

Ernst Federal Science Center for Animal Husbandry, 60, pos. Dubrovitsy, Podolsk District, Moscow Province, 142132 Russia, e-mail kostolan@yandex.ru (✉ corresponding author), melnikovaee@vij.ru,
margaretfornara@gmail.com, bardukv-nikolajj@mail.ru, alex_sermyagin85@mail.ru, gottfried.brem@agrobiogen.de, n_zinovieva@mail.ru

ORCID:
Kostyunina O.V. orcid.org/0000-0001-8206-3221
Sermyagin A.A. orcid.org/0000-0002-1799-6014
Melnikova E.E. orcid.org/0000-0002-7498-1871
Brem G. orcid.org/0000-0002-7522-0708
Fornara M.S. orcid.org/0000-0002-8844-177X
Zinovieva N.A. orcid.org/0000-0003-4017-6863
Bardukov N.V. orcid.org/0000-0002-5497-2409

Received February 18, 2019

 

Integration of DNA markers associated with disease resistance into breeding programs is one of the most promising approaches to control infections of livestock. The identification and implementation of such a marker for the porcine reproductive and respiratory syndrome is particularly topical. The disease causes significant economic losses in the industry, and the proposed vaccines against PRRS are ineffective and associated with a risk of developing viremia after immunization. A promising DNA marker of resistance to this disease is the single nucleotide polymorphism WUR10000125 (WUR) localized in the GBP1 gene. The aim of the study was to assess the reproductive, fattening and meat qualities of Large White and Landrace pigs bred in PRRS-free nucleus farms, considering the genetic variant of the WUR gene. Studies were conducted in 2018-2019 on pigs of Large White and Landrace pigs reared in Selection and Hybrid Center LLC (Voronezh region). Genotypes of 206 sows of Large White and 112 sows of Landrace pig breeds were determined by PCR with using the QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific, USA). The reproductive qualities of sows (number of piglets born alive per litter; total litter weight at birth; number of stillborn pigs per litter; number of mummified pigs per litter; total number born per litter) were estimated based on the first three litters: for Large White pig breed in the period from 2008 to 2018 and for Landrace pig breed in the period from 2010 to 2018. Characteristics of meat and fattening qualities, including the age of 100 kg of body weight, the back-fat thickness, measured in three points, muscle depth (lifetime measurements), were evaluated. To assess the effect of genotype on WUR on the productivity traits the model equations for multivariate analysis of variance were used. The results of animal genotyping showed that the studied pigs were characterized by relatively low frequencies of the “desirable” allele G responsible of resistance to PRRS (2.9 and 13.4 %) and GG genotype (0.49 and 4.46 %) in pigs of Large White and Landrace breeds, respectively. The analysis of values of estimates of the WUR genotypes obtained by the least square means (LSM) method showed a statistically significant superiority of carriers of the AA genotype over animals with AG variant by the total number born per litter, prolificacy and total litter weight at birth in pigs of Large White breed, but the similar tendency in Landrace pigs breed was not found. We noted some superiority of the AA genotype carriers over the AG genotype carriers among sows of Large White breed by EBV of total litter weight at birth. Comparison of meat and fattening parameters did not reveal significant differences either by direct phenotypic estimates or by EBV values. Thus, assessment of the productive traits of Large White and Landrace pigs from PRRS-free nucleus farms did not show a significant effect of the WUR genotypes on the meat and fattening parameters, as well as on the reproductive qualities of Landrace pigs. The increasing of the G allele and GG genotype frequencies under nucleus conditions will lead to an increase in the number of animals with preferable characteristics under PRRS conditions.

Keywords: Sus scrofa, pigs, large white breed, landrace, WUR10000125, reproductive-respiratory syndrome, linear regression, productivity, evaluation of breeding value, DNA marker.

 

REFERENCES

  1. Prajapati B.M., Gupta J.P., Pandey D.P., Parmar G.A., Chaudhari J.D. Molecular markers for resistance against infectious diseases of economic importance. Veterinary World, 2017, 10(1): 112-120 CrossRef
  2. Dietze K., Pinto J., Wainwright S., Hamilton C. Porcine reproductive and respiratory syndrome (PRRS): virulence jumps and persistent circulation in Southeast Asia. In: Focus on... Food and Agriculture Organization of the United Nations, Rome, 2011, Iss. 5: 1-8.
  3. Zimmerman J., Benfield D.A., Murtaugh M.P., Osorio F., Stevenson G.W., Tottemorell M. Porcine reproductive and respiratory syndrome virus (porcine arterivirus). In: Diseases of Swine, 9th Edition. B.E. Straw J.J. Zimmerman, S. D’Allaire, D.J. Taylor (eds.). Blackwell Publishing Professional, Ames, 2006: 387-417.
  4. Rowland R.R., Lunney J., Dekkers J. Control of porcine reproductive and respiratory syndrome (PRRS) through genetic improvements in disease resistance and tolerance. Frontiers in Genetics, 2012, 3: 260 CrossRef
  5. Verkhneva D.A., Semenova N.N. Agrarnyi vestnik Urala, 2012, 10-2(105): 12-13 (in Russ.).
  6. Hurd H.S., Bush E.J., Losinger W., Corso B., Zimmerman J.J., Wills R., Swenson S., Pyburn D., Yeske P., Burkgren T. Outbreaks of porcine reproductive failure: report on a collaborative field investigation. Journal of Swine Health and Production, 2001, 9(3): 103-108.
  7. Mengeling W.L., Lager K.M., Vorwald A.C., Koehler K.J. Strain specificity of the immune response of pigs following vaccination with various strains of porcine reproductive and respiratory syndrome virus. Veterinary Microbiology, 2003, 93(1): 13-24 CrossRef
  8. Charerntantanakul W. Porcine reproductive and respiratory syndrome virus vaccines: immunogenicity, efficacy and safety aspects. World J. Virol., 2012, 1(1): 23-30 CrossRef
  9. Roca M., Gimeno M., Bruguera S., Segalés J., Díaz I., Galindo-Cardiel I.J., Martínez E., Darwich L., Fang Y., Maldonado J., March R., Mateu E. Effects of challenge with a virulent genotype II strain of porcine reproductive and respiratory syndrome virus on piglets vaccinated with an attenuated genotype I strain vaccine. The Veterinary Journal, 2012, 193(1): 92-96 CrossRef
  10. Wang C., Wu B., Amer S., Luo J., Zhang H., Guo Y., Dong G., Zhao B., He H. Phylogenetic analysis and molecular characteristics of seven variant Chinese field isolates of PRRSV. BMC Microbiology, 2010, 10: 146 CrossRef
  11. Wenhui L., Zhongyan W., Guanqun Z., Zhili L., JingYun M., Qingmei X., Baoli S., Yingzuo B. Complete genome sequence of a novel variant porcine reproductive and respiratory syndrome virus (PRRSV) strain: evidence for recombination between vaccine and wild-type PRRSV strains. Journal of Virology, 2012, 86(17): 9543 CrossRef
  12. Lewis C.R.G., Ait-Ali T., Clapperton M., Archibald A.L., Bishop S.C. Genetic perspectives on host responses to porcine reproductive and respiratory syndrome (PRRS). Viral Immunology, 2007, 20(3): 343-358 CrossRef
  13. Gol S., Estany J., Fraile L.J., Pena R.N. Expression profiling of the GBP1 gene as a candidate gene for porcine reproductive and respiratory syndrome resistance. Animal Genetics, 2015, 46: 599-606 CrossRef
  14. Koltes J.E., Fritz-Waters E., Eisley C.J., Choi I., Bao H., Kommadath A., Serão N.V., Boddicker N.J., Abrams S.M., Schroyen M., Loyd H., Tuggle C.K., Plastow G.S., Guan L., Stothard P., Lunney J.K., Liu P., Carpenter S., Rowland R.R., Dekkers J.C., Reecy J.M. Identification of a putative quantitative trait nucleotide in guanylate binding protein 5 for host response to PRRS virus infection. BMC Genomics, 2015, 16: 412 CrossRef
  15. Boddicker N., Waide E.H., Rowland R.R.R., Lunney J.K., Garrick D.J., Reecy J.M., Dekkers J.C.M. Evidence for a major QTL associated with host response to porcine reproductive and respiratory syndrome virus challenge. Journal of Animal Science, 2012, 90(6): 1733-1746 CrossRef
  16. Boddicker N.J., Garrick D.J., Reecy J.M., Rowland B., Lunney J.K., Dekkers J.C.M. Quantitative trait locus on Sus scrofa chromosome 4 associated with host response to experimental infection with porcine reproductive and respiratory syndrome virus. Animal Industry Report, 2013, AS 659: ASL R2823 CrossRef
  17. Boddicker N.J., Bjorkquist A., Rowland R.R.R., Lunney J.K., Reecy J.M., Dekkers J.C.M. Genome-wide association and genomic prediction for host response to porcine reproductive and respiratory syndrome virus infection. Genetics Selection Evolution, 2014, 46: 18 CrossRef
  18. Serão N.V.L., Matika O., Kemp R.A., Harding J.C.S., Bishop S.C., Plastow G.S., Dekkers JC. Genetic analysis of reproductive traits and antibody response in a PRRS outbreak herd. Journal of Animal Science, 2014, 92(7): 2905-2921 CrossRef
  19. Hess A.S., Islam Z., Hess M.K., Rowland R.R.R., Lunney J.K., Wilson A.D., Plastow G.S., Dekkers J.C. Comparison of host genetic factors influencing pig response to infection with two North American isolates of porcine reproductive and respiratory syndrome virus. Genetics Selection Evolution, 2016, 48: 1-20 CrossRef
  20. Waide E.H., Tuggle C.K., Serão N.V.L., Schroyen M., Hess A., Rowland R.R.R., Lunney J.K., Plastow G., Dekkers J.C. Genomewide association of piglet responses to infection with one of two porcine reproductive and respiratory syndrome virus isolates. Journal of Animal Science, 2017, 95: 16-38 CrossRef
  21. Dunkelberger J.R., Serão N.V.L., Niederwerder M.C., Kerrigan M.A., Lunney J.K., Rowland R.R.R., Lunney J.K., Plastow G., Dekkers J.C. Effect of a major quantitative trait locus for porcine reproductive and respiratory syndrome (PRRS) resistance on response to coinfection with PRRS virus and porcine circovirus type 2b (PCV2b) in commercial pigs, with or without prior vaccination for PRRS. Journal of Animal Science, 2017, 95: 584-598 CrossRef
  22. Fornara M.S., Bardukov N.V., Kostyunina O.V., Sadkova Ya.A., Kaz'mina N.A., Zinov'eva N.A. Svinovodstvo, 2018, 5: 17-19 ).
  23. Dunkelberger J., Mathur P.K., Lopes M.S., Knol E.F., Dekkers J.C.M. Pigs can be selected for increased natural resistance to PRRS without affecting overall economic value in the absence of PRRS. Animal Industry Report, 2017, AS 663: ASL R3192 CrossRef
  24. Abella G., Pena R.N., Nogareda C., Armengol R., Vidal A., Moradell L., Tarancon V., Novell E., Estany J., Fraile L. A WUR SNP is associated with European Porcine Reproductive and Respiratory Virus Syndrome resistance and growth performance in pigs. Research in Veterinary Science, 2016, 104: 117-22 CrossRef
  25. Jeon R.L., Putz A.M., Dyck M., Harding J.C.S., Fortin F., Plastow G.S., Kemp B., Dekkers J.C.M. PSIII-10 Effect of WUR genotype on resilience to a polymicrobial natural disease challenge in pigs. Journal of Animal Science, 2019, 97(Supplement_2): 165 CrossRef
  26. Salajpal K., Ðikić M., Karolyi D., Šurina J., Matković M., Liker B. Effect of MC4R polymorphism on physiological stress response in pigs. Poljoprivreda, 2007, 13(1): 46-50.

 

back

 


CONTENTS

 

 

Full article PDF (Rus)

Full article PDF (Eng)