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doi: 10.15389/agrobiology.2019.4.713eng

UDC: 636.033:636.4:636.082.12:577.2

Acknowledgements:
The equipment of the Center for Biological Resources and Bioengineering of Farm Animals (Ernst Federal Science Center for Animal Husbandry)was used for the study.
Supported financially by the Ministry of Education and Science of the Russian Federation, a unique project number RFMEFI60417X0182

 

STUDY OF WUR10000125 POLYMORPHISM ASSOCIATION WITH MEAT, FATTENING AND REPRODUCTIVE TRAITS OF LANDRACE AND LARGE WHITE PIG BREEDS

O.V. Kostyunina, E.E. Melnikova, M.S. Fornara,
N.V. Bardukov, A.A. Sermyagin, G. Brem, N.A. Zinovieva

Ernst Federal Science Center for Animal Husbandry, 60, pos. Dubrovitsy, Podolsk District, Moscow Province, 142132 Russia, e-mail kostolan@yandex.ru (✉ corresponding author), melnikovaee@vij.ru,
margaretfornara@gmail.com, bardukv-nikolajj@mail.ru, alex_sermyagin85@mail.ru, gottfried.brem@agrobiogen.de, n_zinovieva@mail.ru

ORCID:
Kostyunina O.V. orcid.org/0000-0001-8206-3221
Sermyagin A.A. orcid.org/0000-0002-1799-6014
Melnikova E.E. orcid.org/0000-0002-7498-1871
Brem G. orcid.org/0000-0002-7522-0708
Fornara M.S. orcid.org/0000-0002-8844-177X
Zinovieva N.A. orcid.org/0000-0003-4017-6863
Bardukov N.V. orcid.org/0000-0002-5497-2409

Received February 18, 2019

 

Integration of DNA markers associated with disease resistance into breeding programs is one of the most promising approaches to control infections of livestock. The identification and implementation of such a marker for the porcine reproductive and respiratory syndrome is particularly topical. The disease causes significant economic losses in the industry, and the proposed vaccines against PRRS are ineffective and associated with a risk of developing viremia after immunization. A promising DNA marker of resistance to this disease is the single nucleotide polymorphism WUR10000125 (WUR) localized in the GBP1 gene. The aim of the study was to assess the reproductive, fattening and meat qualities of Large White and Landrace pigs bred in PRRS-free nucleus farms, considering the genetic variant of the WUR gene. Studies were conducted in 2018-2019 on pigs of Large White and Landrace pigs reared in Selection and Hybrid Center LLC (Voronezh region). Genotypes of 206 sows of Large White and 112 sows of Landrace pig breeds were determined by PCR with using the QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific, USA). The reproductive qualities of sows (number of piglets born alive per litter; total litter weight at birth; number of stillborn pigs per litter; number of mummified pigs per litter; total number born per litter) were estimated based on the first three litters: for Large White pig breed in the period from 2008 to 2018 and for Landrace pig breed in the period from 2010 to 2018. Characteristics of meat and fattening qualities, including the age of 100 kg of body weight, the back-fat thickness, measured in three points, muscle depth (lifetime measurements), were evaluated. To assess the effect of genotype on WUR on the productivity traits the model equations for multivariate analysis of variance were used. The results of animal genotyping showed that the studied pigs were characterized by relatively low frequencies of the “desirable” allele G responsible of resistance to PRRS (2.9 and 13.4 %) and GG genotype (0.49 and 4.46 %) in pigs of Large White and Landrace breeds, respectively. The analysis of values of estimates of the WUR genotypes obtained by the least square means (LSM) method showed a statistically significant superiority of carriers of the AA genotype over animals with AG variant by the total number born per litter, prolificacy and total litter weight at birth in pigs of Large White breed, but the similar tendency in Landrace pigs breed was not found. We noted some superiority of the AA genotype carriers over the AG genotype carriers among sows of Large White breed by EBV of total litter weight at birth. Comparison of meat and fattening parameters did not reveal significant differences either by direct phenotypic estimates or by EBV values. Thus, assessment of the productive traits of Large White and Landrace pigs from PRRS-free nucleus farms did not show a significant effect of the WUR genotypes on the meat and fattening parameters, as well as on the reproductive qualities of Landrace pigs. The increasing of the G allele and GG genotype frequencies under nucleus conditions will lead to an increase in the number of animals with preferable characteristics under PRRS conditions.

Keywords: Sus scrofa, pigs, large white breed, landrace, WUR10000125, reproductive-respiratory syndrome, linear regression, productivity, evaluation of breeding value, DNA marker.

 

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