Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2018, Vol. 53, ¹ 4, p. 762-768.
(how to cite this article)

doi: 10.15389/agrobiology.2018.4.762eng

UDC 639.3.034.2

Acknowledgements:
This work was carried out using devices of Unique Scientific Installation Module Complex (USI-MC, SSC RAS) and Bio-resource Collection of Rare and Endangered Fish Species (SSC RAS) ¹ 73602.

Supported financially by Federal Program of Russian State Academies for 2013-2020, State Registration No 01201354245 (0256-2018-0007), and by the grant of the President of the Russian Federation (ÌÊ-115.2017.11)

 

REPRODUCTION OF RUSSIAN STURGEON (Acipenser gueldenstaedtii) VIABLE JUVENILES USING CRYOPRESERVED SPERM AND
BEHAVIORAL REACTIONS OF THE CRYO-PROGENY

A.A. Krasilnikova1, A.M. Tikhomirov2

1Federal Research Centre Southern Scientific Center RAS, Federal Agency of Scientific Organizations, 41, ul. Chekhova, Rostov-on-Don, 344006 Russia,
e-mail alexandra.kras@yandex.ru (✉ corresponding author);
2Astrakhan State Technical University, 16, ul. Tatishcheva, Astrakhan, 414056 Russia, e-mail tikhomirov41@mail.ru

ORCID:
Krasilnikova A.A. orcid.org/0000-0002-7186-8849
Tikhomirov A.M. orcid.org/0000-0002-4087-818X
The authors declare no conflict of interests

Received June 9, 2016

 

Cryopreservation of male reproductive cells is an important issue of genetic biodiversity conservation strategy and the development of fisheries and aquaculture. The use of cryopreserved semen in artificial reproduction and aquaculture will provide genetically diverse progeny, reduce area and the cost of maintaining male fishes, and, thereby, will allow rise in female herd abundance. Cryopreserved sperm can be used at any time, without risk of untimely maturing or improper quality. The data on the low-temperature preservation indicates that the long-term storage in liquid nitrogen does not significantly affect the safety of cells after freezing and thawing. The viability of cryopreserved sperm is usually confirmed by laboratory fertilization of caviar, without further observations of the obtained juveniles. This paper reports for the first time the behavioral response of pre-larvae, larvae, and juveniles of Russian sturgeon (Acipenser gueldenstaedtii von Brandt & Ratzeburg 1833), obtained with the use of frozen-thawed sperm, and shows physiological fullness of the resulting progeny. The aim of this work was to compare quality of Russian sturgeon offspring as influenced by cryopreserved and native semen. In using frozen-thawed sperm stored 2 years in liquid nitrogen, the conception was 50 % compared to 80 % in the control insemination with native semen. The mortality rate for the whole period was 7 and 5 % in the test and the control groups, respectively. The obtained cryo-progeny was viable. One-day old pre-larvae, 8-day old larvae and 15-day old juveniles of the test group were superior in size and weight as compared to the control group. Behavioral responses of the offspring were evaluated in «open field» test which was carried out individually by placing an individual (pre-larvae, larvae, juveniles) in a special installation with coordinate grid. The pre-larvae activity was somewhat, but not significantly, higher in cryo-progeny. No differences were found between 8-day old larvae in the response to irritants, except bright light (ð ≤ 0.05). In 15-day old juveniles, the response was adequate in both groups. Basal activity and reactivity differed significantly (ð ≤ 0.05) in both groups. So, cryopreserved sperm led to some morphometric advantage in the progeny compared to the individuals produced by conventional methods, and some advantages were found in the response to irritants, however, on the whole, the differences were not significant. A small alteration may be due to the difference in frozen cell subpopulations. Thus, frozen semen contributes to young fish vitality and may be recommended for artificial reproduction and aquaculture.

Keywords: sturgeons, Russian sturgeon, Acipenser gueldenstaedtii, sperm, cryopreservation, cryo-progeny, pre-larvae, larvae, juveniles, behavior.

 

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REFERENCES

  1. Worm B., Barbier E.B., Beaumont N., Duffy J.E., Folke C., Halpern B.S., Jackson J.B.C., Lotze H.K., Micheli F., Palumbi S.R., Sala E., Selkoe K.A., Stachowicz J.J., Watson R. Impacts of biodiversity loss on ocean ecosystem services. Science, 2006, 314: 787-790 CrossRef
  2. Béné Ch., Arthur R., Norbury H., Allison E.H., Beveridge M., Bush S., Campling L., Leschen W., Little D., Squires D., Thilsted S.H., Troell M., Williams M. Contribution of fisheries and aquaculture to food security and poverty reduction: assessing the current evidence. World Dev., 2016, 79: 177-196 CrossRef
  3. Ottinger M., Clauss K., Kuenzer C. Aquaculture: relevance, distribution, impacts and spatial assessments — a review. Ocean Coast. Manage., 2016, 119: 244-266 CrossRef
  4. Bronzi P., Rosenthal H., Gessner J. Global sturgeon aquaculture production: an overview. J. Appl. Ichthyol., 2011, 27: 169-175 CrossRef
  5. Safina C., Duckworth A. Fish conservation. In: Encyclopedia of biodiversity (second edition). S.A. Levin (ed.). Princeton University, New Jersey, USA Academic Press, 2013: 443-455 CrossRef
  6. Olesen I., Rosendal G.K., Tvedt M.W., Bryde M., Bentsen H.B. Access to and protection of aquaculture genetic resources — structures and strategies in Norwegian aquaculture. Aquaculture, 2007, 1: S47-S61 CrossRef
  7. Gjedrem T., Robinson N., Rye M. The importance of selective breeding in aquaculture to meet future demands for animal protein: a review. Aquaculture, 2012, 350-353: 117-129 CrossRef
  8. Duncan N.J., Sonesson A.K., Chavanne H. Principles of finfish broodstock management in aquaculture: control of reproduction and genetic improvement. In: Advances in aquaculture hatchery technology. A volume in Woodhead Publishing Series in Food Science, Technology and Nutrition. G. Allan, G. Burnell (eds.). Woodhead Publishing, Cambridge, 2013: 23-75 CrossRef
  9. Cabrita E., Sarasquete C., Martínez-Páramo S., Robles V., Beirão J., Pérez-Cerezales S., Herráez M.P. Cryopreservation of fish sperm: applications and perspectives (review). J. Appl. Ichthyol., 2010, 26(5): 623-635 CrossRef
  10. Herráez P., Cabrita E., Robles V. Fish gamete and embryo cryopreservation: state of the art. In: Aquaculture biotechnology. G.L. Fletcher, M.L. Rise (eds.). Wiley-Blackwell, 2011: 303-317 CrossRef
  11. Tsvetkova L.I., Pronina N.D., Dokina O.B., Rekubratskii A.V., Parnyshkov V.A. Voprosy rybolovstva, 2012, 13(3-51): 538-545 (in Russ.).
  12. Labbé C., Robles V., Herraez M.P. Cryopreservation of gametes for aquaculture and alternative cell sources for genome preservation. In: Advances in aquaculture hatchery technology. A volume in Woodhead Publishing Series in Food Science, Technology and Nutrition. G. Allan, G. Burnell (eds.). Woodhead Publishing, Cambridge, 2013: 76-116 CrossRef
  13. Kopeika J., Thornhill A., Khalaf Y. The effect of cryopreservation on the genome of gametes and embryos: Principles of cryobiology and critical appraisal of the evidence. Hum. Reprod., 2015, 21(2): 209-227 CrossRef
  14. Robles V., Cabrita E., Kohli V., Herráez M.P. Prospects and development in fish sperm and embryo cryopreservation. In: Aquaculture research progress. T.K. Nakamura (ed.). Nova Science Publishers, Inc., NY, 2009: 199-210.
  15. Krasil'nikova A.A. Sovershenstvovanie protsessa kriokonservatsii reproduktivnykh kletok samtsov ryb. Avtoreferat kandidatskoi dissertatsii [Improvement of cryopreservation of male reproductive cells of fish. PhD Thesis]. Astrakhan', 2015 (in Russ.).
  16. Sampath Kumar J.S., Betsy C.J. Cryopreservation of fish gametes and its role in enhancing aquaculture production. In: Advances in marine and brackishwater aquaculture. S. Perumal, A.R. Thirunavukkarasu, P. Pachiappan (eds.). Springer, New Delhi, 2015: 241-246 CrossRef
  17. Tsai S., Lin C. Advantages and applications of cryopreservation in fisheries science. Braz. Arch. Biol. Tech., 2012, 55(3): 425-434 CrossRef
  18. Ponomareva E.N., Krasil'nikova A.A., Tikhomirov A.M., Firsova A.V. Yug Rossii: ekologiya, razvitie, 2016, 11(1): 59-68 CrossRefv (in Russ.).
  19. Krasil'nikova A.A., Tikhomirov A.M. Estestvennye nauki, 2014, 2: 62-69 (in Russ.).
  20. Krasilnikova A.A., Tikhomirov A.M. Vestnik Astrakhanskogo gosudarstvennogo tekhnicheskogo universiteta. Seriya Rybnoe khozyaistvo, 2014, 2: 72-78 (in Russ.).
  21. Krasil'nikova A.A., Tikhomirov A.M. Estestvennye nauki, 2015, 3(52): 105-111 (in Russ.).
  22. Aramli M.S., Nazari R.M. Motility and fertility of cryopreserved semen in Persian sturgeon, Acipenser persicus, stored for 30-60 min after thawing. Cryobiology, 2014, 69(3): 500-502 CrossRef
  23. Dzyuba B., Boryshpolets S., Cosson J., Dzyuba V., Fedorov P., Saito T., Psenicka M., Linhart O., Rodina M. Motility and fertilization ability of sterlet Acipenser ruthenus testicular sperm after cryopreservation. Cryobiology, 2014, 69(2): 339-341 CrossRef
  24. Nynca J., Dietrich G.J., Dobosz S., Grudniewska J., Ciereszko A. Effect of cryopreservation on sperm motility parameters and fertilizing ability of brown trout semen. Aquaculture, 2014, 433: 62-65 CrossRef
  25. Chebanov M.S., Galich E.V., Chmyr' Yu.N. Rukovodstvo po razvedeniyu i vyrashchivaniyu osetrovykh ryb [Guide to breeding and growing sturgeon]. Moscow, 2004: 148 (in Russ.).
  26. Vitvitskaya L.V., Nikonorov S.I., Tikhomirov A.M., Kozlov A.V. V sbornike: Fundamental'nye nauki — narodnomu khozyaistvu [In: Fundamental sciences for the national economy]. Moscow, 1990: 123-149 (in Russ.).
  27. Nikonorov S.I., Vitvitskaya L.V. Ekologo-geneticheskie problemy iskusstvennogo vosproizvodstva osetrovykh i lososevykh ryb [Ecogenetic aspects of artificial reproduction of sturgeons and salmonids]. Moscow, 1993 (in Russ.).
  28. Ivanter E.V., Korosov A.V. Vvedenie v kolichestvennuyu biologiyu [Introduction to quantitative biology]. Petrozavodsk, 2011 (in Russ.).
  29. Shmal'gauzen I.I. Osnovy sravnitel'noi anatomii [Basic principles of comparative anatomy]. Moscow, 1947 (in Russ.).
  30. Abdurakhmanov G.M., Zaitsev V.F., Lozhnichenko O.V., Fedorova N.N., Tikhonova E.YU., Lepilina I.N. Razvitie zhiznenno vazhnykh organov osetrovykh v rannem ontogeneze [The development of vital organs in early ontogeny of sturgeon]. Moscow, 2006 (in Russ.). 
  31. Ponomareva E.N., Nevalennyi A.N., Belaya M.M., Krasil'nikova A.A. Vestnik Astrakhanskogo gosudarstvennogo tekhnicheskogo universiteta. Seriya Rybnoe khozyaistvo, 2017, 4: 118-127 CrossRef(in Russ.).  

 

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