Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2018, Vol. 53, ¹ 4, p. 842-850.
(how to cite this article)

doi: 10.15389/agrobiology.2018.4.842eng

UDC 636.52/.58:619:578:575

Acknowledgements:
The study was carried out with the equipment of «Biotechnology» center (All-Russian Research Institute of Agricultural Biotechnology).
Supported financially by Federal Agency of Scientific Organizations, the state assignment for Breeding and Genetic Center Smena ¹ 007-01359-17-00 in the framework of the Federal Scientific and Technical Program of agricultural development for 2017-2025, Subprogram «Development of competitive national broiler meat cross»

 

DETECTION OF AVIAN LEUKEMIA VIRUS SUBGROUP K IN RUSSIA
AND ITS MOLECULAR GENETIC ANALYSIS

À.Ì. Borodin1, Ya.I. Alekseev2,3, N.V. Konovalova2,3,
Å.V. Terentyeva2,3, D.N. Efimov4, Zh.V. Emanuilova4, S.V. Smolov4,
Î.À. Ogneva4, V.I. Fisinin5

1Non-proffn Partnership Institute of Medico-Biological Research, 10, ul. Studenaya, Nizhnii Novgorod, 603000 Russia, e-mail Aborodinm@sinn.ru;
2All-Russian Research Institute of Agricultural Biotechnology, Federal Agency of Scientific Organizations, 42, ul. Ti-miryazevskaya, Moscow, 127550 Russia, e-mail jalex@iab.ac.ru (✉ corresponding author);
3LLC Syntol, 42, ul. Timiryazevskaya, Moscow, 127550 Russia, e-mail jalex@syntol.ru;
4Breeding and Genetic Center Smena, Federal Agency of Scientific Organizations, pos. Bereznyaki, Moscow Province, 141327 Russia, e-mail Smena@tsinet.ru, dmi40172575@yandex.ru (✉ corresponding author);
5Federal Scientific Center All-Russian Research and Technological Poultry Institute RAS, Federal Agency of Scientific Organizations,10, ul. Ptitsegradskaya, Sergiev Posad, Moscow Province, 141315 Russia, e-mail olga@vnitip.ru, vnitip@vnitip.ru

ORCID:
Borodin À.Ì. orcid.org/0000-0002-1478-1261
Emanuilova Zh.V. orcid.org/0000-0002-8855-2947
Alekseev Ya.I. orcid.org/0000-0002-1696-7684
Smolov S.V. orcid.org/0000-0001-6058-3672
Konovalova N.V. orcid.org/0000-0003-4316-1077
Ogneva Î.À. orcid.org/0000-0002-8698-1975
Terentyeva Å.V. orcid.org/0000-0003-2777-0948
Fisinin V.I. orcid.org/0000-0003-0081-6336
Efimov D.N. orcid.org/0000-0002-4152-2476
The authors declare no conflict of interests

Received November 27, 2017

 

The Avian leukemia virus (ALV) belongs to the genus Alpharetrovirus (Retroviridae). The genome of the virus is a single-stranded RNA of more than 7,000 nucleotides in length. The ALV subgroups A, B, C, D, J, K and E are specific viruses of chicken. ALV classification is based on the type-specific envelope protein antigens, GP85. ALV is widely spread all over the world, causes various diseases, reduces productivity and leads to huge damage to poultry industry. The viruses of subgroup K were first discovered in Asian countries. Studies of the prevalence of ALV of subgroup K are few and have not been conducted in Russia before. Our goal was to study the spread of the ALV of this subgroup to the chickens of Russian poultry farms using a test system designed to identify the genome of the ALV subgroup K by real-time PCR and analysis of the properties of the ALV of subgroup K. The test of the real-time PCR test system was carried out on 5292 DNA samples of chickens of domestic broiler meat type of one of the farms in the Moscow Province and in chickens of meat and egg breeds from various regions of Russia. The ALV-K-specific gene gp85 sequences were found in 177 (3.3 %) broilers of one of the farms in the Moscow Province. Sequencing of gp85 gene fragments revealed the presence of two groups of different ALV subgroup K in the chickens of this farm: one had 96 % similarity to the ALV subgroup K strains Oki 009, GDFX0601, GDFX0602, GDFX0603, Km_5845 etc., and the other - up to 100 % similar to ALV subgroup K strain TW-3593. Using the reference sequence of the ALV subgroup K strain GD14LZ (KU605774), 22 full-length genomic sequences, belonging to ALV subgroup K, with 95 to 100 % similarity to the GD14LZ gp85 gene sequences were detected in GenBank. The construction of phylogenetic trees based on the gp85 gene sequences showed that subgroup K viruses form a separate group, distinct from the rest of the viruses, which can be divided into four clusters based on differences in long terminal repeats. The subgroup K viruses have a set of four different 3´UTR sequences belonging to both the pathogenic ALV subgroup J and the less pathogenic ALV subgroup E, and there are also ALV K and Oki, the ALV-K-specific 3´UTRs. In addition to the Moscow region, ALV subgroup K was found in the Kaliningrad, Leningrad, Sverdlovsk, and Novgorod regions of Russia. Thus, the distribution of ALV subgroup K is not limited to the countries of Asia. Scientific publications show that ALV subgroup K can cause gliomas and myocarditis. Literature data allows us to say that ALV subgroup K can be pathogenic and theses viruses need a control program, because even the subclinical form of exogenous and endogenous ALV can lead to large economic losses.

Keywords: avian leukosis virus subgroup K, real time PCR, ALV subgroup K detection.

 

Full article (Rus)

Full article (Eng)

 

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