Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2017, V. 52, № 4, pp. 812-819
(how to cite this article)

doi: 10.15389/agrobiology.2017.4.812eng

UDC 636.32/.38:591.05:577.11(470.44/.47)

 

HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS IN EDILBAI
LAMBS SUBJECTED TO PHARMACOLOGICAL CORRECTION
OF HYPOMICROELEMTNROSIS DUE TO BIOGEOCHEMICAL
CONDITIONS OF THE LOWER VOLGA REGION

V.I. Vorobiov, D.V. Vorobiov, E.N. Scherbakova, I.I. Hismetov

Astrakhan State University, 1, pl. Shaumyana, Astrakhan, 414001 Russia,
e-mail veterinаria-2011@mail.ru (corresponding author)

The authors declare no conflict of interests

ORCID:

Vorobiov V.I.
orcid.org/0000-0002-6669-2850

Scherbakova E.N.
orcid.org/0000-0001-6141-554X

Vorobiov D.V.
orcid.org/0000-0002-4745-8866

Hismetov I.I.
orcid.org/0000-0002-0948-5994

Received May 12, 2016

 

In recent years, latent hypomicroelementoses are increasingly diagnosed in a variety of farm animals, including sheep, in Russia, EU, India and other countries (V.T. Samokhin, 2008). The deficit of selenium, iodine and other vital elements leads to a decrease in hematological parameters and a change in the activity of free radical oxidation and oxidant protection which is a fundamental molecular and cellular mechanism of pathogenesis at various hypomicroelementoses (T.N. Rodionova et al., 2010). The aim of the paper was to comprehensively survey the biogeochemical situation in the Lower Volga region and to evaluate the influence of Se and I deficiency on the physiological status of Edilbai lamb not subjected to pharmacological correction of hypomicroelementoses and after administration of Sedimine® (Russia), a metabolism-regulating organic composition containing Se, I and Fe (III)—dextran stabilizing complex. The probes of soil and water, plants, sheep organs and tissues were sampled according to V.V. Kowalski (1974). Trace elements were detected using atomic absorption spectrometry (M.E. Britske, 1982), selenium was assayed fluorimetrically according to V.V. Yermakov (1975), and iodine was analyzed in thiocyanate-nitrate test. To quantitate the products of lipid peroxidation in lamb blood, we estimated diene conjugates by UV absorption spectra at 233 nm (Z. Platzer, 1970), malondialdehyde — according to V.S. Buzlama et al. (1997), catalase activity — as per M.L. Korolyuk (1980) and glutathione peroxidase — as described by R. Paglia et al. (1967). Hematological parameters were evaluated according to I.P. Kondrakhin et al. (2004). It was shown that the basic components of terrestrial ecosystems in the Lower Volga region are low in selenium, iodine and cobalt relative to the Black Earth region (V. Kowalski, 1974). In the intact lambs, negative balances of iodine and selenium were found during the observation. Also, hematological parameters and antioxidant defense were low. Blood and metabolic parameters together with the balance of studied microelements indicated a development of latent combined selenium and iodine hypomicroelementosis in the lambs. The use of organic product Sedimine resulted in a significant (Р < 0.05) correction of the syndrome of selenium and iodine deficiency in Edilbai lambs. Indeed, the blood levels of selenium increased by 84.0 % and iodine — by 92.7 %, the number of erythrocytes was 15.6 % higher, hemoglobin was 7.8 % higher, Se, I, globulins were 8.76 % higher, while the amount of glucose reduced by 33.9 %. The diene conjugates diminished by 28 %, the malonaldehyde — by 8.2 %, the catalase activity increased by 43.3 %, and glutathione peroxidase activity was 39.4 % higher. The four month-aged lambs which received Sedimine showed the improvement in all the studied metabolic parameters and the weight gain which exceeded that in the control by 14.4 % (Р < 0.05). Additionally, after the Sedimine application the lamb meat was superior to that of the control animals in accumulation of physiologically important I, Se, Mn, Cu, and Co.

Keywords: Edilbai sheep, lambs, biogeochemical provinces, microelements, Se, I, hypomicroelementosis, lipid peroxidation, antioxidant defense, hematological indexes.

 

Full article (Rus)

Full text (Eng)

 

REFERENCES

  1. Samokhin V.T. Materialy VI Mezhdunarodnoi biogeokhimicheskoi shkoly AGTU «Biogeokhimiya v narodnom khozyaistve: fundamental'nye osnovy noosfernykh tekhnologii» [Proc. VI Int. Biogeochtmical School AGTU «Biogeochemisry: fundamental aspects of noosphere-based technologies»]. Astrakhan', 2008: 159-160 (in Russ.).
  2. Rodionova T.N., Antipov V.A., Lazarev V.G. Farmakologiya selenoorganicheskogo preparata DAFS-25 i ego ispol'zovanie v zhivotnovodstve i veterinarii [DAFS-25, a selenium-contactingcomposition: pharmacological parameters and application in animal husbandry and veterinary medicine]. Saratov, 2010 (in Russ.).
  3. Vorob'ev V.I., Vorob'ev D.V. Zhurnal fundamental'nykh i prikladnykh issledovanii, 2010, 1(30): 12-18 (in Russ.).
  4. Vorob'ev D.V., Vorob'ev V.I., Kutepov A.Yu., Polkovnichenko A.P. Fiziologicheskii status i ego korrektsiya u zhvachnykh, vseyadnykh zhivotnykh i ptits v biogeokhimicheskikh usloviyakh regiona Nizhnei Volgi [Physiological parameters and their improving in ruminants, other mammalians and poultry under biogeochemical conditions of Lower Volga]. St. Petersburg, 2011 (in Russ.).
  5. Alhidary I.A., Shini S., Al Jassim R.A.M., Gaughan J.B. Effect of various doses of injected selenium on performance and physiological responses of sheep to heat load. Journal of Animal Science, 2012, 90(9): 2988-2994 CrossRef
  6. Humann-Ziehanka E., Renkob K., Muellerc A. S., Roehriga P., Wolfsena J., Gantera M. Comparing functional metabolic effects of marginal and sufficient selenium supply in sheep. Journal of Trace Elements in Medicine and Biology, 2013, 27(4): 380-390 CrossRef
  7. Sejian V., Singh A.K., Sahooand S.A., Naqvi M.K. Effect of mineral mixture and antioxidant supplementation on growth, reproductive performance and adaptive capability of Malpura ewes subjected to heat stress. Journal of Animal Physiology and Animal Nutrition, 2014, 98(1): 72-83 CrossRef
  8. Faix S., Faixova Z., Boldizarova K., Javorsky P. The effect of long-term high heavy metal intake on lipid peroxidation of gastrointestinal tissue in sheep. Vet. Med. — Szech, 2005, 50(9): 401-405.
  9. Mohebbi-Fani M., Mirzaei A., Nazifi S., Shabbooie Z. Changes of vitamins A, E, and C and lipid peroxidation status of breeding and pregnant sheep during dry seasons on medium-to-low quality forages. Tropical Animal Health and Production, 2012, 44(80): 259-265 CrossRef
  10. Heidarpour M., Mohri M., Borji H., Moghaddas E. Oxidant/antioxidant balance and trace elements status in sheep with liver cystic echinococcosis. Original Article Comparative Clinical Pathology, 2013, 22: 1043-1049 CrossRef
  11. Xina G.S., Longa R.J., Guob X.S., Irvineb J., Dingb L.M., Dingb L.L., Shanga Z.H. Blood mineral status of grazing Tibetan sheep in the Northeast of the Qinghai—Tibetan Plateau. Livestock Science, 2011, 136: 102-107.
  12. Saleha M.A., Al-Salahyb M.B., Sanousic S.A. Corpuscular oxidative stress in desert sheep naturally deficient in copper. Small Ruminant Research, 2008, 80: 33-38.
  13. Esmaeilnejada B., Tavassolia M., Asri-Rezaeib S., Dalir-Naghadehb B., Malekinejadc H., Jalilzadeh-Aminb G., Arjmanda J., Golabia M., Hajipoura N. Evaluation of antioxidant status, oxidative stress and serum trace mineral levels associated with Babesia ovis parasitemia in sheep. Veterinary Parasitology, 2014, 1-2: 38-45 CrossRef
  14. Chadio St.E., Kotsampasi B.M., Menegatos J.G., Zervas G.P., Kalogiannis D.G. Effect of selenium supplementation on thyroid hormone levels and selenoenzyme activities in growing lambs. Biological Trace Element Research, 2006, 109(2): 145-154 CrossRef
  15. Qin Sh., Gao J., Huang K. Effects of different selenium sources on tissue selenium concentrations, blood GSH-Px activities and plasma interleukin levels in finishing lambs. Biological Trace Element Research, 2007, 116(1): 91-102 CrossRef
  16. Bolanda T.M., Brophya P.O., Callana J.J., Quinna P.J., Nowakowskib P., Crosby T.F. The effects of mineral supplementation to ewes in late pregnancy on colostrum yield and immunoglobulin G absorption in their lambs. Livestock Production Science, 2005, 97(2-3): 141-150 CrossRef
  17. Koyuncu M., Yerlikaya H. Short sommunication effect of selenium-vitamin E injections of ewes on reproduction and growth of their lambs. South African Journal of Animal Science, 2007, 37(4): 233-236 CrossRef
  18. Kumar N., Garg A.K., Mudgal V., Dass R.Sh., Chaturvedi V.K., Varsh-
    ney V.P. Effect of different levels of selenium supplementation on growth rate, nutrient utilization, blood metabolic profile, and immune response in lambs. Biological Trace Element Research, 2008, 126(1): 44-56 CrossRef
  19. Kumara N., Garga A.K., Dassa R.S., Chaturvedib V.K., Mudgala V., Varshneyc V.P. Selenium supplementation influences growth performance, antioxidant status and immune response in lambs. Animal Feed Science and Technology, 2009, 153(1-2): 77-87 CrossRef
  20. Vorob'ev D.V. Fundamental'nye issledovaniya, 2011, 11: 402-406 (in Russ.).
  21. Solov'ev V.N., Firsov A.A., Filov V.A. Farmakokinetika [Pharmacokinetics]. Moscow, 1980 (in Russ.).
  22. Koval'skii V.S. Geokhimicheskaya ekologiya [Geochemical ecology]. Moscow, 1974 (in Russ.).
  23. Tomme M.F. Metodika izucheniya perevarimosti kormov i ratsionov [Feeds and diets: methods to study digestibility]. Moscow, 1965 (in Russ.).
  24. Kondrakhin I.P., Arkhipov A.V., Levchenko V.I., Talanov G.A., Fro-
    lov A.A., Novikov V.E. Metody veterinarnoi klinicheskoi laboratornoi diagnostiki [Clinical diagnostics in veterinary medicine]. Moscow, 2004 (in Russ.).
  25. Platser Z., Vidlakova M., Kupila L. Chekhosl. med. obzor, 1970, 16(1): 30-34 (in Russ.).
  26. Buzlama V.S., Titov Yu.T., Vostroilova G.A., Vashchenko Yu.E. Ekspress-biotest. Biologicheskii monitoring system [Express biotest. Biomonitoring in different systems]. Voronezh, 1997 (in Russ.).
  27. Korolyuk M.A. Laboratornoedelo, 1980, 1: 40-59 (in Russ.).
  28. Paglia R., Valentine J. Definition of a glutathione peroxidases. Lab. Clin. Med., 1967: 158-169.
  29. Britske M.E. Atomno-absorbtsionnyi spektrokhimicheskii analiz [Atomic absorption spectroscopy]. Moscow, 1982 (in Russ.).
  30. Ermakov V.V. Vitaminy. Biokhimiya vitamina E i selena, 1975, 8: 142-146 (in Russ.).
  31. Yavich P.A., Kakhetelidze M.B., Churadze L.I. Issledovaniya v oblasti estestvennykh nauk, 2014, 1. Available http://science.snauka.ru/2014/01/6585. No date (in Russ.).
  32. Matveev A.M., Pavlovskii V.A., Prokhorova N.V. Ekologicheskie osnovy akkumulyatsii tyazhelykh metallov sel'skokhozyaistvennymi rasteniyami [Ecological aspects of heavy metal accumulation in cultivated crops]. Samara, 1997 (in Russ.).
  33. Kanapin K. Edil'baevskaya ovtsa [Edilbay sheep]. Almaty, 2009 (in Russ.).
  34. Bellof G., Most E., Pallauf J. Concentration of copper, iron, manganese and zinc in muscle, fat and bone tissue of lambs of the breed German Merino Landsheep in the course of the growing period and different feeding intensities. Journal of Animal Physiology and Animal Nutrition, 2007, 91(3-4): 100-108 CrossRef
  35. Humann-Ziehanka E., Gantera M., Hennig-Paukaa I., Binderb A. Trace mineral status and liver and blood parameters in sheep without mineral supply compared to local roe deer (Capreolus capreolus) populations. Small Ruminant Research, 2008, 75(2-3): 185-191 CrossRef
  36. Lankin V.Z., Tikhaze A.K., Belenkov Yu.N. Svobodnoradikal'nye protsessy v norme i pri patologicheskikh sostoyaniyakh [Free radicals in health and disease]. Moscow, 2001 (in Russ.).
  37. Cheng J., Ma H., Fan C., Zhang Z., Jia Z., Zhu X., Wang L. Effects of different copper sources and levels on plasma superoxide dismutase, lipid peroxidation, and copper status of lambs. Biological Trace Element Research, 2011, 144(1): 570-579 CrossRef

back