Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2016, V. 51, № 4, pp. 516-523.

doi: 10.15389/agrobiology.2016.4.516eng

UDC 619:616-099:612.017:577.124.8:57.084.1

Acknowledgements:
The authors thank the staff of the Omsk Regional Veterinary Laboratory for assistance in the experiments.

 

ADAPTIVE AND COMPENSATORY RESPONSES IN RATS AT THE EARLY
STAGES OF AN ACUTE INTOXICATION WITH DELTAMETHRIN

T.V. Gerunov1, E.A. Chigrinskii2, Yu.N. Fedorov3, L.K. Gerunova1,
V.D. Konvai1

1P.A. Stolypin Omsk State Agricultural University, 1, Institutskaya pl., Omsk, 644008 Russia,
e-mail vsed@mail.ru;
2Omsk State Medical University, Russian Federation Ministry of Health, 12, ul. Lenina, Omsk, 644043 Russia,
e-mail chigrinski@list.ru;
3All-Russian Research and Technological Institute of Biological Industry, Federal Agency of Scientific Organizations, 17, pos. Biokombinata, Shchelkovskii Region, Moscow Province, 141142 Russia, e-mail fun181@mail.ru

Received May 23, 2016

 

The world market of synthetic pyrethroids is estimated more than at 2.5 bn. dollars and will grow in the next years. Numerous works of domestic and foreign authors are devoted to toxicology of pyrethroids, however the issues related to pathogenesis of an acute poisoning of animals with pesticides of that group as well as the principles of laboratory diagnostics at an early stage of intoxication and pathogenetic therapy at poisoning warrant further investigations. For many years deltamethrin has been successfully used in plant growing as well as in animal husbandry creating a poisoning hazard in case of violation of treatment regulations. Morbidity and clinical outcome depend largely on severity of functional endocrine and immune systems disorders. The purpose of the study was to determine dynamics of adaptive and compensatory reactions in animals at an early stage of an acute intoxication with deltamethrin. In experiment white laboratory rats as an established mammal model in biomedical research were used. The experiment was performed on male rats (weight of 180-200 gr) arranged in 6 groups (of 10-12 rats each). Animals from groups II, IV and VI have been subjected to an acute peroral intoxication with deltamethrin (Butox 50, Intervet, Netherlands) in a dose of 43.5 mg/kg of body weight. Rats of groups I, III and V served as control. Rats from different groups were put out of the experiment sequentially: I and II — in a day; III and IV — in three days; V and VI — in a week after the beginning of the experiment. Glucose content was estimated in whole blood of rats, and concentration of insulin and corticosterone was assayed in blood serum. Pieces of animal timus were fixed in 4 % neutral formaldehyde, dehydrated in alcohols with the increasing concentration and embedded into paraffin. Histologic sections at 3-5 microns in thickness were made with the rotational microtome and stained with haematoxylin and eosin, and also according to Van-Gie-zon. For identification of mast cells the histologic sections were stained with Bismarck brown to Shubich. Increase in blood corticosterone, insulin and glucose levels by 46.3 (p = 0.0001), 31.9 (p = 0.0139) and 25.6 % (p = 0.0052), respectively, was found in a day after poisoning with deltamethrin. On day 3 after poisoning the blood concentration of corticosterone and glucose in rats remained high along with a decrease in insulin content. Hypocorticosteronemia and hypoglycemia were observed on day 7 after poisoning, with the insulin level close to control values. Corticosterone and glucose content has been reduced by 17.3 (p = 0.0407) and 19.8 % (p = 0.0267), respectively, compared to control. Photomicrographs showed a reduction in the number of thymocytes, activation of apoptosis, an increase in quantity of mastocytes along with an intensification of degranulation as well as development of haemodynamic disorders in thymus when poisoning rats. In a week after intoxication the thymocytes level was partially restored. No animal died during experiment which evidences the efficiency of adaptation and compensatory mechanisms in experimental rats, including hypothalamic-pituitary thymic system. Results of our researches make a contribution to understanding compensatory and adaptive mechanisms under experimental intoxication, and allow estimating functional capabilities of body systems when developing pharmacological correction at poisonings.

Keywords: pesticides, deltamethrin, corticosterone, insulin, glucose, thymus, rats, intoxication.

 

Full article (Rus)

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REFERENCES

  1. Pyrethroids market by type (Bifenthrin, Deltamethrin, Permethrin, Cypermethrin, Cyfluthrin, and Lambda-cyhalothrin), by crop type (cereals & grains, oilseeds & pulses, and fruits & vegetables) & by region — global trends & forecasts to 2019. MarketsandMarkets, 2015. Available http://www.marketsandmarkets.com/Market-Reports/pyrethroids-pesticide-market-170901303.html. Accessed May 06, 2016.
  2. Shafer T.J., Meyer D.A., Crofton K.M. Developmental neurotoxicity of pyrethroids insecticides: critical review and future research needs. Environ. Health Perspect., 2005, 113: 123-136 CrossRef
  3. Moser V.C., Stewart N., Freeborn D.L., Crooks J., MacMillan D.K., Hedge J.M., Wood C.E., McMahen R.L., Strynar M.J., Herr D.W. Assessment of serum biomarkers in rats after exposure to pesticides of different chemical classes. Toxicol. Appl. Pharmacol., 2015, 282(2): 161-174 CrossRef
  4. Eraslan G., Bilgili A., Essiz D., Akdogan M., Sahindokuyucu F. The effects of deltamethrin on some serum biochemical parameters in mice. Pestic. Biochem. Phys., 2007, 87(2): 123-130.
  5. Sharma P., Singh R., Jan M. Dose-dependent effect of deltamethrin in testis, liver, and kidney of Wistar rats. Toxicol. Int., 2014, 21(2): 131-139 CrossRef
  6. Nwozo S., Akpodono E., Oyinloye B. Plasma, erythrocyte membrane bound enzymes and tissue histopathology in male Wistar rats exposed to common insecticides. J. Pestic. Sci., 2015, 40(1): 13-18 CrossRef
  7. Meditsinskaya toksikologiya: natsional'noe rukovodstvo /Pod redaktsiei E.A. Luzhnikova [Medical toxicology: the national guidance. E.A. Luzhnikov (ed.)]. Moscow, 2012 (in Russ.).
  8. Zemskov A.M., Zemskov V.M., Zoloedov V.I., Bzhozovskii E.E. Uspekhi sovremennoi biologii, 2003, 123(2): 138-146 (in Russ.).
  9. Sengupta P. The laboratory rat: relating its age with human's. Int. J. Prev. Med., 2013, 4(6): 624-630.
  10. Lilley R. Pathohistological techniques and practical histochemistry [Russian translation]. V.V. Portugalov (ed.). Moscow, 1969 (in Russ.).
  11. Biokhimiya /Pod redaktsiei E.S. Severina [Biochemistry. E.S. Severin (ed.)]. Moscow, 2015 (in Russ.).
  12. Chandra A.K., Ghosh R., Chatterjee A., Sarkar M. Amelioration of vanadium-induced testicular toxicity and adrenocortical hyperactivity by vitamin E acetate in rats. Mol. Cell. Biochem., 2007, 306(1): 189-200 CrossRef
  13. Titov V.N. Klinicheskaya meditsina, 2014, 5: 18-28 (in Russ.).
  14. Goncharov N.P., Kolesnikova D.S. Kortikosteroidy: metabolizm, mekhanizm deistviya i klinicheskoe proyavlenie [Corticosteroids: metabolism, mechanism of action and clinical manifestation]. Moscow, 2002 (in Russ.).
  15. Dupre J. Regulation of the secretions of the pancreas. Ann. Rev. Med., 1970, 21: 299-316.
  16. Brownlee M. The pathobiology of diabetic complications: a unifying mechanism. Diabetes, 2005, 54(6): 1615-1625 CrossRef
  17. Boden G., Cheung P., Stein T.P., Kresge K., Mozzoli M. FFA cause hepatic insulin resistance by inhibiting insulin suppression of glycogenolysis. Am. J. Physiol. Endocrinol. Metab., 2002, 283(1): E12-E19 CrossRef
  18. Martel C., Melner M.H., Gagne D., Simard J., Labrie F. Widespread tissue distribution of steroid sulfatase, 3b-hydroxysteroid dehydrogenase/Δ5-Δ4isomerase(3β-HSD), 17β-HSD5α-reductase and aromatase activities in the rhesus monkey. Mol. Cell. Endocrinol., 1994, 104(1): 103-111 CrossRef
  19. Aitken R.J., Roman S.D. Molecular mechanisms in spermatogenesis. Newcastle, Landes Bioscience and Springer Sciense + Business media, 2008.
  20. Chigrinski E.A., Conway V.D., Metrinskiy Y.Y. Ascorbate level in reproductive organs of male rats in acute deltamethrin intoxication. European Journal of Natural History, 2014, 4: 30.
  21. Chigrinski E.A., Conway V.D., Gerunova L.K., Gerunov T.V. Glutathione-related enzyme activity in rats’ testes and epididymis at an acute intoxication with a synthetic pyrethroid deltamethrin. International Journal of Pharma and Bio Sciences, 2015, 6(4): B340-B344.
  22. Gerunov T.V., Chigrinskii E.A., Gerunov V.I., Konvai V.D. Vestnik APK Stavropol'ya, 2015, S1: 44-48 (in Russ.).
  23. Abdel-Daim M.M., El-Ghoneimy A. Synergistic protective effects of ceftriaxone and ascorbic acid against subacute deltamethrin-induced nephrotoxicity in rats. Renal Failure, 2015, 37(2): 297-304 CrossRef
  24. Xu M.Y., Wang P., Sun Y.J., Wang H.P., Liang Y.J., Zhu L., Wu Y.J. Redox status in liver of rats following subchronic exposure to the combination of low dose dichlorvos and deltamethrin. Pestic. Biochem. Physiol., 2015, 124: 60-65 CrossRef
  25. Gerunova L.K., Gerunov V.I., Dovgan' N.B., Kozina A.Yu. Insektitsidy adonis i sumi-al'fa: mikst- i monotoksichnost' (eksperimental'nye issledovaniya) [Insecticides Adonis and Sumi-alfa: mixed and mono toxicity — experimental study]. Omsk, 2008 (in Russ.).
  26. Kalinina M.N., Ketlinskii S.A., Okovityi S.V., Shulenin S.N. Zabolevaniya immunnoi sistemy [Diseases of the immune system]. Moscow, 2008 (in Russ.).
  27. Litvinova L.S., Seledtsov V.I., Shupletsova V.V., Gutsol A.A., Anishchenko E.S. Vestnik Rossiiskogo gosudarstvennogo universiteta im. I. Kanta, 2011, 1: 77-86 (in Russ.).
  28. Kiseleva N.M., Inozemtsev A.N. Immunopatologiya, allergologiya, infektologiya, 2010, 2: 13-20 (in Russ.).
  29. Gerunov T.V., Red'kin Yu.V., Gerunova L.K. Uspekhi sovremennoi biologii, 2011, 131(5): 474-482 (in Russ.).

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