UDC 636.52/.58:591.3:591.05

doi: 10.15389/agrobiology.2014.4.86eng


V.Yu. Titov1, O.V. Kosenko1, G.V. Kondratov2

1All-Russian Research and Technological Institute of Poultry, Russian Academy of Agricultural Sciences,
10, ul. Ptitse-gradskaya, Sergiev Posad, Moscow Province, 141300 Russia,
e-mail vtitov43@yandex.ru;
2K.I. Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology,
23, ul. Akademika Skryabina, Moscow, 109472 Russia,
e-mail kazagor@gmail.com

Received March 19, 2014

It is known that nitric oxide (NO) mediates the regulation of smooth muscle tone in the blood vessels and the gastrointestinal tract, the central and autonomic nervous systems activity, differentiation and apoptosis in tissues, immune response, and gene expression in animals and humans. This is a short-lived substance oxidized rapidly to nitrate and nitrite. Embryogenesis is associated with the synthesis of nitric oxide (NO) and accumulation of its metabolites in embryo. Nevertheless, the mechanism of this relationship is unknown. In this paper we reported the results of comparing dynamics of the NO metabolite levels in the parts of and a whole embryo in the layers and broiler chicks. Incubated eggs were examined in the breeds (Andalusian Blue, Yurlovskaya Golosistaya, Golubaya Myasoyaichnaya, Cochin White, Brahma Dark, Brahma Pale, P-11, Cornish, Plymouth Rock, Malay fighting hens, Uzbek fighting hens), some lines (Х1, Х2, Х12, Х34 of Hysex White cross, B5, B6 and B56 of Cornish, B7, B9, and B79 of Plymouth Rock), and four crosses (SP 789, Hysex White, Smena 8, Cobb 500). The NO metabolites were detected using developed enzymatic sensor. The test is based on catalase inhibition by nitrite, nitrosothiols (RSNO), Fe-dinitrosyl complex and nitro derivatives of high-molecular compounds (RNO2) in the presence of halide ions, repressed by the factors different for each class of compounds. The close correlation was observed between meat productivity of adult poultry and NO metabolites accumulation in different embryo parts. The NO metabolite accumulation differed up to 100 times in meat- and egg-hen embryos, and in meat-egg hens and mini-hens the intermediate parameters were indicated. It was shown that nitrate as a final metabolic product was accumulated in the muscle of embryo. Due to time when the most intensive accumulation occurs in ontogenesis (3 days), some factor rather than the muscle itself, but influencing muscle histogenesis could be considered as the main agent involved in NO metabolism. Nitric oxide as a mediator can either activate this agent, or affect its synthesis.

Keywords: nitric oxide, embryogenesis, meat productivity.


Full article (Rus)



1. Ignarro L. Biological actions and properties of endothelium-derived nitric oxide formed and released from artery and vein. Circulation Research, 1989, 65: 1-21. CrossRef
2. Moncada S., Palmer R., Higgs E. Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacological Reviews, 1991, 43: 109-142.
3. Stalmer J., Singel D., Loscalzo J. Biochemistry of nitric oxide and its redox-activated forms. Science, 1992, 258:1898-1902.
4. Li J., Billiar T., Talanian R., Kim Y. Nitric oxide reversibly inhibits seven members of the caspase family viaS-nitrosylation. Biochem. Biophys. Res. Commun.,1997, 240:419-424. CrossRef
5. Kim Y.-M., Chung H-T., Simmons R., Billiar T. Cellular nonheme iron content is a determinant of nitric oxide-mediated apoptosis, necrosis, and caspase inhibition. J. Biol. Chem., 2000, 275: 10954-10961.
6. Ischiropoulos H., Zhu L., Beckman J. Peroxynitrite formation from macrophage-derived nitric oxide. Arch. Biochem. Biophys., 1992, 298: 446-451. CrossRef
7. Zhou J., Brune B. NO and transcriptional regulation: from signaling to death. Toxicology, 2005, 208: 223-233. CrossRef
8. Severina I., Bussygina O., Pyatakova N., Malenkova I., Vanin A. Activation of soluble guanylate cyclase by NO donors—Snitrosothiols, and dinitrosyl-iron complexes with thiol-containing ligands. Nitric Oxide,2003, 8: 155-163.
9. Vanin A. Dinitrosyl iron complexes with thiolate ligands: physicochemistry, biochemistry and physiology. Nitric Oxide,2009, 21: 1-13.
10. Lima E., Bonini M., Augusto O., Barbeiro H., Souza H., Abdalla D. Nitrated lipids decompose to nitric oxide and lipid radicals and cause vasorelaxation. Free Radic. Biol. Med.,2005, 39: 532-539. CrossRef
11. Battaglia C., Ciottii P., Notarangelo L., Fratto R., Facchinetti F., De Aloysio D. Embryonic production of nitric oxide and its role in implantation: a pilot study. J. Assisted Reproduction and Genetics, 2003, 20: 449-454. CrossRef
12. Retsky M.I., Shakhov A.G., Bliznetzova G.N., Artemieva S.S., Kave-
rin N.N., Ermakova N.V., Kosmatych, N.A. Age dynamics of formation of nitric oxide at the large cattle. Russian Agricultural Science, 2004, 30: 58-60.
13. Titov V.Yu., Vinnikova E.Z., Akimova N.S., Fisinin V.I. Nitric oxide (NO) in bird embryogenesis: physiological role and ability of practical use. World’s Poultry Science Journal, 2012, 68: 83-95.
14. Titov V.Yu., Akimova N.S., Vinnikova E.Z., Fisinin V.I. Doklady RASKHN, 2009, 35: 47-48.
15. Titov V.Yu., Petrenko Yu.M., Vanin A.F. Mechanism of inhibition of catalase by nitro and nitroso compounds. Biochemistry (Moscow), 2008, 73: 92-96. CrossRef
16. Titov V.Yu., Petrenko Yu.M., Vanin A.F., Stepuro I.I. Biofizika, 2010, 55: 95-106.
17. Titov V.Yu., Petrenko Yu.M., Vanin A.F. Klinicheskaya laboratornaya diagnostika, 2009, 9: 6-14.
18. Stamler J., Meissner G. Physiology of nitric oxide in skeletal muscle. Physiol Rev., 2001, 81: 209-237.
19. Lee H., Baek M., Moon K., Song W., Chung Ch., Ha D, Kang M.-S. Nitric oxide as a messenger molecule for myoblast fusion. J. Biol. Chem., 1994, 269: 14371-14374.
20. Cazzato D., Assi E., Moscheni C., Brunelli S., De Palma C., Cervia D., Perrotta C., Clementi E. Nitric oxide drives embryonic myogenesis in chicken through the upregulation of myogenic differentiation factors. Exp. CellRes. 2014, 320: 269-80. CrossRef