doi: 10.15389/agrobiology.2013.4.53eng

UDC 639.3.09:619:578:616-079.7:577.083.3


I.B. Prokaeva1, B.V. Novikov2

1All-Russian Research Institute of Veterinary Virology and Miсrobiology, Russian Academy of Agricultural Sciences,
Pokrov, Petushinskii Region, Vladimir Province, 601120 Russia,
2N.F. Gamaleya Research Institute of Epidemiology and Microbiology,
18, ul. Gamalei, Moscow, 123098 Russia

Received January 25, 2013

Herpesvirus disease of Siberian sturgeon, caused by SbSHV, is especially severe in the industrial populations. The commonly used method for its specific diagnostics has a sufficient disadvantage because of SbSHV active reproduction in vivo when the water temperature is in the range of 10-18 °С, and therefore, can be applied mainly in spring and sometimes in autumn. At other seasons the amount of the virus in the fish tissues reduces so that it becomes nearly unrevealable. We have developed a method for retrospective diagnosis of herpesvirus disease of Siberian sturgeon using indirect ELISA version. Experimental data on ELISA development for the detection of antibodies against Siberian sturgeon herpesvirus are presented. Procedures for preparing the ELISA specific reagents are described, including the virus reproduction in cell culture, isolation of IgM-like immunoglobulin from hyperimmune antisera of sturgeon, etc. The effectiveness of the proposed method and the reaction of neutralization was compared. It is shown, that by elaborated method the SbSHV antibodies can be detected both in the Siberian sturgeon, and among closely related species of sturgeon, or their hybrids.

Keywords: herpesvirus of the Siberian sturgeon, immunoglobulin, ELISA, serodiagnostics.


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1. Gerpesvirusnaya bolezn' sibirskogo osetra [Herpesvirus Disease of Siberian Sturgeon].
2. Shchelkunov I.S. Virusnye infektsii u osetrovykh ryb. Rybnoe khozyaistvo. Analiticheskaya i referativnaya informatsiya. Seriya: Bolezni gidrobiontov v akvakul'ture [Fish Industry. Analytical and Reference Information. Series: Diseases of Hydrobionts in Aquaculture]. Moscow, VNIERKH, 2000, v. 1: 3-16.
3. Hedrick R.P., McDowell T.S., Groff J.M., Yun S., Wingfield W.F. Characteristics of two viruses isolated from white sturgeon, Acipenser transmontanus. Proc. of Second International Symposium on viruses of lower vertebrates /J.L. Fryer (ed.). Corvallis, OR, Oregon State University Press, 1991: 165-174.
4. Kalabekov I.M., Kalabekova F.S., Shchelkunova T.I., Shchelkunov I.S., Kolbasov D.V. Mat. 3-i Mezhdunarodnoi konferentsii «Problemy immunologii, patologii i okhrany zdorov'ya ryb» [Proc. III Int. Conference «Problems of Fish Immunity, Pathology and Health Protection»]. Borok-Moscow, 2011: 303-305.
5. Shchelkunova T.I., Kupinskaya O.A., Mashchenko N.A., Shchelkunov I.S. Tezisy dokadov Pervogo kongressa ikhtiologov Rossii [Abstracts of Papers, I Congress of Russian Ichthyologists]. Astrakhan', 1997: 302-303.
6. Hedrick R.P., McDowell T.S., Groff J.M., Yun S., Wingfield W.H. Isolation of epitheliotropic herpesvirus from white sturgeon Acipenser transmontanus. Diseases of Aquatic Organisms, 1991, 11(1): 49-56.
7. Bourne F.J. Ig A immunoglobulin from porcine serum. BBRC, 1969, 36: 138-142.
8. Gel-filtration. Principles and methods. Amersham Bioscience AB, 2002.
9. Ion exchange chromatography. Principles and methods. Amersham Bioscience AB, 2002.
10. Immunologicheskie metody /Pod red. G. Frimelya [Immunological Methods /G. Frimel' (ed.)]. Moscow, 1987.
11. Lowry O.H., Farr A.J., Paudall R.Y. Protein measurement with the Folin phenol reagent. J. Biol. Chem., 1951, 193: 265-275.
12. Sbornik instruktsii po bor'be s boleznyami ryb. Chast'.1 [Instructions for the Control of Fish Diseases. Part I]. Moscow, 1998: 60-113.
13. Wilson M.B., Nakane P.K. Recent development in the periodate method of conjugating horseradish peroxidase (HPRO) to antibodies. In: Immunofluorescence and related staining techniques /W. Knapp et al. (eds.). Elsevier/North Holland Biomedical Press, Amsterdam, 1978: 215-244.