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Bogdanova E.M., Bertova A.D., Kirpichnikova A.A., Biktasheva M.O., Kondratieva A.V., Shapiro A.S., Puzanskiy R.K., Korotenko T.L., Mukhina Z.M., Yemelyanov V.V., Shishova M.F. Growth and viability of coleoptiles under oxygen deficiency in Oryza sativa L. from the collection of the Federal rice research center. Sel'skokhozyaistvennaya Biologiya [Agricultural Biology], 2023, Vol. 58, № 3, p. 538-553.
(how to cite this article)

doi: 10.15389/agrobiology.2023.3.538eng

UDC: 633.18:581.1:57.04

Acknowledgements:
The equipment of the Research Park “Center for Molecular and Cell Technologies” at St. Petersburg State University was used.
Supported financially by the Russian Science Foundation, grant No. 22-14-00096, https://rscf.ru/en/project/22-14-00096

 

GROWTH AND VIABILITY OF COLEOPTILES UNDER OXYGEN DEFICIENCY IN Oryza sativa L. FROM THE COLLECTION OF THE FEDERAL RICE RESEARCH CENTER

E.M. Bogdanova1, A.D. Bertova1, A.A. Kirpichnikova1,
M.O. Biktasheva1, A.V. Kondratieva1, A.S. Shapiro1,
R.K. Puzanskiy1,2, T.L. Korotenko3, Z.M. Mukhina3,
V.V. Yemelyanov1, M.F. Shishova1 ✉

1Saint-Petersburg State University, 7-9, Universitetskaya nab., St. Petersburg, 199034 Russia, e-mail bogdanova.ekaterina15@gmail.com, tasiabertova@gmail.com, nastin1972@mail.ru, togepi03@mail.ru, ann.knd17@gmail.com, al.shapiro@bk.ru, bootika@mail.ru, mshishova@mail.ru (✉ corresponding author);
2Komarov Botanical Institute RAS, 2, ul. Professora Popova, St. Petersburg, 197022 Russia, e-mail puzansky@yandex.ru;
3Federal Rice Research Center, 3, Belozernii, Krasnodar, Russia 350921, e-mail korotenko.tatyan@mail.ru, agroplazma@gmail.com

ORCID:
Bogdanova E.M. orcid.org/0009-0005-6092-8462
Puzanskiy R.K. orcid.org/0000-0002-5862-2676
Bertova A.D. orcid.org/0009-0005-9774-6689
Korotenko T.L. orcid.org/0000-0002-3831-4879
Kirpichnikova A.A. orcid.org/0000-0001-5133-5175
Mukhina Z.M. orcid.org/0000-0003-3557-1615
Biktasheva M.O. orcid.org/0009-0000-9263-7815
Yemelyanov V.V. orcid.org/0000-0003-2323-5235
Kondratieva A.V. orcid.org/0009-0005-3688-3372
Shishova M.F. orcid.org/0000-0003-3657-2986
Shapiro A.S. orcid.org/0009-0002-2345-1958

Final revision received March 27, 2023
Accepted April 14, 2023

The distinctive ability of rice seedlings lies in the ability to germinate under conditions of oxygen lack. At the germination stage, the first to develop is the coleoptile, a juvenile organ that protects the true leaf in cereals. The mechanisms of regulation of growth and development of this organ have not been studied to a large extent. Special attention may be paid to a coleoptile in seedlings capable of germinating under oxygen conditions. In the presented study, for the first time, the importance of the growth rate and viability of coleoptiles of rice seedlings during flooding in determining survival and development was demonstrated. A total of 36 varieties and forms from the collection of the Federal Research Center for Rice, Krasnodar, were tested. Sprint and Kuban 3 were among the fastest growing varieties. Their coleoptiles reached 19-25 mm length both under normoxia and hypoxia. The slowest growing group included three Philippine varieties harbouring the SUB1A allele (HHZ11 Y6-Y2-SUB1, HHZ8 SAL 14 SUB1, HHZ9 DT12 SUB1), Chinese variety Xiannui and domestic varieties Amethyst, Zhemchug, Natasha, Rapan and Yuzhnaya noch. This group of varieties was characterized by inhibition of the growth of coleoptiles by 2.5-3 times under submergence. In main a positive correlation was estimated between the coleoptile length in normoxia and hypoxia ), (p = 0.70, p = 10-6), i.e. forms that actively grow in an aerobic environment also grow rapidly when flooded. Further detailed analysis of the growth of coleoptiles under hypoxic conditions showed that growth changes correspond to several patterns. The most common reaction to hypoxia in rice coleoptiles of the first group was growth suppression, but with the preservation of a small part of the plants that continue to grow. This group included all Philippine cultivars harbouring the SUB1A allele. In the second group, a significant proportion was plants which length under submergence exceeded that at normoxia. Approximately half of the plants of the third group slowed growth arrest, while the other part continued to grow as in normoxia. The fourth group included the variety Sprint, which was the only one to have traits of avoidance strategy (LOES — low oxygen escape syndrome) associated with growth enhancement, although this enhancement was not intensive. The variety Yuzhnaya noch had a unique growth pattern, the coleoptiles of which grew slowly both in normoxia and being submerged. In addition to growth, the viability of coleoptiles was analyzed in the work, which was assessed using a tetrazolium test. Under hypoxic conditions, the viability of all tested forms significantly decreased. In the fastest growing varieties (Sprint, Kuban 3), the color intensity of tetrazolium salts was higher both in the control (6-fold) and in the experiment (2-fold), compared to slow growing forms (Amethyst, Yuzhnaya noch, Philippine SUB1A varieties). In general, growth rate correlates with metabolic rate and submergence tolerance. Obtained results illustrate the tolerance to oxygen deficiency of the genotypes from the collection of the Federal Research Center of Rice, and show that coleoptile elongation can be used as a criterion for assessing the tolerance of rice varieties to the lack of oxygen.

Keywords: Oryza sativa, rice, submergence, hypoxia, coleoptile, growth, tolerance.

 

REFERENCES

  1. Voesenek L.A.C.J., Bailey-Serres J. Flooding tolerance: O2 sensing and survival strategies. Current Opinion in Plant Biology, 2013, 16(5): 647-653 CrossRef
  2. Mommer L., Visser E.J.W. Underwater photosynthesis in flooded terrestrial plants: a matter of leaf plasticity. Annals of Botany, 2005, 96(4): 581-589 CrossRef
  3. Bailey-Serres J., Voesenek L.A.C.J. Flooding stress: acclimations and genetic diversity. Annual Review of Plant Biology, 2008, 59: 313-339 CrossRef
  4. Polko J.K., Voesenek L.A.C.J., Peeters A.J.M., Pierik R. Petiole hyponasty: an ethylene-driven, adaptive response to changes in the environment. AoB PLANTS, 2011, 2011: plr031 CrossRef
  5. Voesenek L.A.C.J., Bailey-Serres J. Flood adaptive traits and processes: an overview. New Phytologist, 2015, 206(1): 57-73 CrossRef
  6. Chirkova T., Yemelyanov V. The study of plant adaptation to oxygen deficiency in Saint Petersburg University. Biological Communications, 2018, 63(1): 17-31 CrossRef
  7. Yemelyanov V.V., Shishova M.F. The role of phytohormones in the control of plant adaptation to oxygen depletion. In: Phytohormones and abiotic stress tolerance in plants. N. Khan, R. Nazar, N. Iqbal, N. Anjum (eds.). Springer, Berlin, Heidelberg, 2012: 229-248 CrossRef
  8. Hartman S., Sasidharan R., Voesenek L.A.C.J. The role of ethylene in metabolic acclimations to low oxygen. New Phytologist, 2021, 229(1): 64-70 CrossRef
  9. Hattori Y., Nagai K., Furukawa S., Song X.-J., Kawano R., Sakakibara H., Wu J., Matsumoto T., Yoshimura A., Kitano H., Matsuoka M., Mori H., Ashikari M. The ethylene response factors SNORKEL1 and SNORKEL2 allow rice to adapt to deep water. Nature, 2009, 460: 1026-1030 CrossRef
  10. Winkel A., Pedersen O., Ella E., Ismail A.M., Colmer T.D. Gas film retention and underwater photosynthesis during field submergence of four contrasting rice genotypes. Journal of Experimental Botany, 2014, 65(12): 3225-3233 CrossRef
  11. Bailey-Serres J., Fukao T., Gibbs D.J., Holdsworth M.J., Lee S.C., Licausi F., Perata P., Voesenek L.A.C.J., van Dongen J.T. Making sense of low oxygen sensing. Trends in Plant Science, 2012, 17(3): 129-138 CrossRef
  12. Bailey-Serres J., Lee S.C., Brinton E. Waterproofing crops: effective flooding survival strategies. Plant Physiology, 2012, 160(4): 1698-1709 CrossRef
  13. Schmitz A.J., Folsom J.J., Jikamaru Y., Ronald P., Walia H. SUB1A-mediated submergence tolerance response in rice involves differential regulation of the brassinosteroid pathway. New Phytologist, 2013, 198(4): 1060-1070 CrossRef
  14. Fukao T., Xu K., Ronald P.C., Bailey-Serres J. A variable cluster of ethylene response factor-like genes regulates metabolic and developmental acclimation responses to submergence in rice. The Plant Cell, 2006, 18(8): 2021-2034 CrossRef
  15. Takahashi H., Sato K., Takeda K. Mapping genes for deep-seeding tolerance in barley. Euphytica, 2001, 122: 37-43 CrossRef
  16. Rebetzke G.J., Zheng B., Chapman S.C. Do wheat breeders have suitable genetic variation to overcome short coleoptiles and poor establishment in the warmer soils of future climates? Functional Plant Biology, 2016, 43(10): 961-972 CrossRef
  17. Brown P.R., Singleton G.R., Tann C.R., Mock I. Increasing sowing depth to reduce mouse damage to winter crops. Crop Protection, 2003, 22(4): 653-660 CrossRef
  18. O’Sullivan P.A., Weiss G.M., Friesen D. Tolerance of spring wheat (Triticum aestivum L.) to trifluralin deep-incorporated in the autumn or spring. Weed Research, 1985, 25(4): 275-280 CrossRef
  19. Schillinger W.F., Donaldson E., Allan R.E., Jones S.S. Winter wheat seedling emergence from deep sowing depths. Agronomy Journal, 1998, 90(5): 582-586 CrossRef
  20. Bovill W.D., Hyles J., Zwart A.B., Ford B.A., Perera G., Phongkham T., Brooks B.J., Rebetzke G.J., Hayden M.J., Hunt J.R., Spielmeyer W. Increase in coleoptile length and establishment by Lcol-A1, a genetic locus with major effect in wheat. BMC Plant Biology, 2019, 19: 332 CrossRef
  21. Luo H., Hill C.B., Zhou G., Zhang X.-Q., Li C. Genome-wide association mapping reveals novel genes associated with coleoptile length in a worldwide collection of barley. BMC Plant Biology, 2020, 20: 346 CrossRef
  22. Ma M., Cen W., Li R., Wang S., Luo J. The molecular regulatory pathways and metabolic adaptation in the seed germination and early seedling growth of rice in response to low O2 stress. Plants, 2020, 9(10): 1363 CrossRef
  23. Korotenko T.L., Sadovskaya L.L. Trudy Kubanskogo gosudarstvennogo agrarnogo universiteta, 2018, 72: 202-206 CrossRef (in Russ.).
  24. Korotenko T.L., Chizhikova S.S., Pustovalov R.A. Byulleten’ Gosudarstvennogo Nikitskogo botanicheskogo sada, 2019, 133: 174-181 CrossRef (in Russ.).
  25. Yemelyanov V.V., Lastochkin V.V., Chirkova T.V., Lindberg S.M., Shishova M.F. Indoleacetic acid levels in wheat and rice seedlings under oxygen deficiency and subsequent reoxygenation. Biomolecules, 2020, 10(2): 276 CrossRef
  26. R Core Team. R: The R Project for Statistical Computing. Available: https://www.r-project.org/. Accessed: 02/26/2023.
  27. Huang S., Shingaki-Wells R.N., Petereit, J., Alexova R., Millar A.H. Temperature-dependent metabolic adaptation of Triticum aestivum seedlings to anoxia. Scientific Reports, 2018, 8: 6151 CrossRef
  28. Pucciariello C. Molecular mechanisms supporting rice germination and coleoptile elongation under low oxygen. Plants, 2020, 9(8): 1037 CrossRef
  29. Blokhina O., Fagerstedt K.V. Oxidative metabolism, ROS and NO under oxygen deprivation. Plant Physiology and Biochemistry, 2010, 48(5): 359-373 CrossRef
  30. Yemelyanov V.V., Lastochkin V.V., Prikaziuk E.G., Chirkova T.V. Activities of catalase and peroxidase in wheat and rice plants under conditions of anoxia and post-anoxic aeration. Russian Journal of Plant Physiology, 2022, 69(6): 117 CrossRef
  31. Chirkova T.V., Novitskaya L.O., Blokhina O.B. Lipid peroxidation and antioxidant systems under anoxia in plants differing in their tolerance to oxygen deficiency. Russian Journal of Plant Physiology, 1998, 45(1): 55-62.
  32. Shikov A.E., Lastochkin V.V., Chirkova T.V., Mukhina Z.M., Yemelyanov V.V. Post-anoxic oxidative injury is more severe than oxidative stress induced by chemical agents in wheat and rice plants.Acta Physiologiae Plantarum, 2022, 44(9): 90 CrossRef
  33. Emel’yanov V.V., Lastochkin V.V., Chirkova T.V., Slyusarenko A. Materialy dokladov VII S»ezda fiziologov rasteniy Rossii «Fiziologiya rasteniy — fundamental’naya osnova еkologii i innovatsionnykh biotekhnologiy». Nizhniy Novgorod, 4-10 iyulya 2011. Chast’ I /Pod redaktsiey Vl.V. Kuznetsova, A.P. Veselova, G.A. Romanova [Proc. of the 7th Congress of plant physiologists of Russia «Plant physiology is the fundamental basis of ecology and innovative biotechnologies». Nizhny Novgorod, July 4-10, 2011. Part I. Vl.V. Kuznetsov, A.P. Veselov, G.A. Romanov (eds.)]. Nizhniy Novgorod, 2011: 236-237 (in Russ.).
  34. Yemelyanov V.V., Lastochkin V.V., Chirkova T.V. The role of polyamines in signaling and adaptation to oxygen deprivation and subsequent re-aeration in plants. Proceedings of 4th International symposium «Plant Signaling & Behavior». St. Petersburg, 19-24 June 2016. V. Demidchik, O. Voitsekhovskaja, E. Tyutereva, G. Pozhvanov (eds.). SINEL Co.Ltd., SPb, 2016: 107.

 

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