doi: 10.15389/agrobiology.2019.3.597eng

UDC: 633.11:632.4.01/.08:577:57.082.5



N.S. Zhemchuzhina, M.I. Kiseleva, A.I. Zhemchuzhina, S.A. Elizarova

All-Russian Research Institute of Phytopathology, 5, ul. Institute, pos. Bol’shie Vyazemy, Odintsovskii Region, Moscow Province, 143050 Russia, e-mail (✉ corresponding author),,,

Zhemchuzhina N.S.
Zhemchuzhina A.I.
Kiseleva M.I.
Elizarova S.A.

Received July 5, 2018


The State Collection of phytopathogenic microorganisms (ARRIP) accumulates a great number of wheat leaf rust agent (Puccinia triticina Eriks.) isolates, an extremely harmful and epiphytotic pathogen. Annually the collection is replenished with new leaf rust isolates from different populations. Annual estimation of the virulence genes’ frequency in isolates makes it possible to track the dynamics of the fungal populations. One of the main tasks of the State collection is to preserve the isolates of the fungus without losing their biological properties to involve these isolates in further laboratory and field experiments. For this purpose, the viability and virulence of P. triticina collection isolates was evaluated during 10-year preservation in a household refrigerator (+4 °С) and in a REVCO freezer (-80 °С, Revco, USA). We used 124 P. triticina isolates collected in 2005, 2006, 2008, 2009, 2010 and 2012 from damaged wheat (Triticum aestivum L.) samples in the Central, North Caucasus and West Siberian regions of the Russian Federation. The isolates differed in virulence and were assigned to 74 phenotypes. The viability of the isolates after storage was determined by spore germination on 2 % water agar plates and by inoculation of susceptible wheat cultivars. Storing of the leaf rust uredospores at low positive temperatures quickly led to a weakening of the viability of the fungus, up to complete destruction. After 1-2 months at +4 °С, the isolates had a high germination capacity, from 48 to 95 %, which decreased in 6 months to 3.0-22.7 %. The correlation between the number of germinated spores on 2 % agar-agar and the duration of storage at a low positive temperature was 0.79. Leaf rust isolates remained viable during storage for 3-10 years under low negative temperatures (-80 °С). The number of germinated spores of different isolates regardless of the preservation period was 25-79 %, and the disease intensity reached 25-100 %. Many factors influence viability of isolates. These mostly are improper sample processing prior to putting into storage and during reviving from an anabiotic state, or disturbance of storage of technical character. However, storage of P. triticina isolates at low negative temperatures ensured a rather high survival rate for 10 years. Checking the virulence of the isolates after 7-year storage with the use of susceptible wheat cultivar and Lr9 and Lr19 lines showed identical indicators before and after the storage. The method of storing spores at -80 °C allows rather high rate of preservation without changing fungal viability and virulence.

Keywords: collection of microorganisms, leaf rust, isolate, population, virulence, preservation.



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