Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2018, Vol. 53, ¹ 3, p. 547-556.
(how to cite this article)

doi: 10.15389/agrobiology.2018.3.547eng

UDC 633.812:631.527.5:581.192:577.13:665.53

Acknowledgments:
Supported financially by Russian Science Foundation (grant ¹ 14-5000079)

 

INTERSPECIFIC HYBRIDIZATION IN LAVANDIN
(Lavandula × intermedia Emeric ex Loisel.) BREEDING FOR ESSENTIAL
OIL QUALITY

V.D. Rabotyagov, A.E. Palii, Yu.S. Khokhlov

Nikita Botanical Gardens — National Scientific Center RAS, Federal Agency for Scientific Organizations, 52, ul. Nikitskii spusk, pgt Nikita, Yalta, Republic of Crimea, 298648 Russia, e-mail onlabor@yandex.ru, runastep@mail.ru (✉ corresponding author), aomor@mail.ru

ORCID:
Rabotyagov V.D. orcid.org/0000-0002-0139-5089
Khokhlov Yu.S. orcid.org/0000-0001-8591-9208
Palii A.E. orcid.org/0000-0002-5234-3393

Received April 12, 2018

 

Lavandin cultivars (Lavandula × intermedia Emeric ex Loisel.) are sterile interspecies hybrids Lavandula angustifolia Mill. × L. latifolia Medic. They are of great interest for the essential oil industry. Lavandin cultivars express 1.5-2-fold higher yields of raw biomass and essential oil production, as well as 4-fold higher essential oil yield per area unit as compared to the used lavender cultivars. World production of lavandin essential oil is 1200 thousand tons, and lavender oil production is only 200 thousand tons. However, the quality of lavandin essential oil is lower compared to lavender one because of camphor, 1,8-cineole and borneol significant amounts. Besides, it is impossible to use lavandin cultivars in further breeding works, as they are sterile. The main trend in lavandin breeding is to improve the essential oil quality by reducing unwanted components to a minimum. In order to create lavandin hybrids characterized by high essential oil quality we had first synthesized tetraploid forms of L. angustifolia and L. latifolia and then crossed these forms with each other. As the result, their sterility was overcome and amphidiploid hybrids were obtained. Those hybrids were further used to create new highly effective cultivars. Crossing between amphidiploid hybrid ¹ 48 and lavender cultivars (Belyanka, Record, Prima) let us to select cross combinations and create lavandin cultivars with minimum amount of camphor, borneol and 1,8-cineol. It was revealed that obtained plants often demonstrated intermediate when compared to their parental forms. Thus, initial forms with high content of linalool and linalyl acetate and lower content of unwanted compounds should be used in breeding works. In cross combinations Amphidiploid 48 × Belyanka, Lavandin hybrids with high content of linalool (up to 68.8 %) were derived. Some hybrids had the content of borneol (up to 0.5 %), camphor (1.9 %) and 1,8-cineole (1.8 %) similar to that of parental forms. In cross combination Amphidiploid 48 × Prima hybrids were obtained with high content of linalool (up to 57.9 %) and linalyl acetate (up to 32.8 %) and low content of camphor (0.2 %), borneol (1.6 %) and 1,8-cineole (up to 0.9 %). The results of our investigations demonstrated that it is possible to create Lavandin hybrids with borneol amount lower than in the original forms. Camphor and 1,8-cineole content depression was not beyond the intermediate type of inheritance. We suppose that the extremely low content of 1,8-cineole and camphor in the lavender chemotypes selected for breeding corresponds to their lower biological limit of these metabolites. Hybrid plants (L. × intermedia) can only approximate these characteristicsof L. angustifolia. Lavandin hybrids with the best essential oil composition have been obtained by crossings between the most closely related, from a biological point of view, chemotypes with dominant alleles of linalool and linalyl acetate and recessive alleles of camphor and 1,8-cineole in L. angustifolia, which is possible under creating Lavandin hybrids with two genomes of L. angustifolia and one genome of L. latifolia.

Keywords: allotriploid, Lavandin, Lavandula × intermedia Emeric ex Loisel., hybrid, essential oil composition.

 

Full article (Rus)

Full article (Eng)

 

REFERENCES

  1. Lis-Balchin M. Lavander. The genus Lavanda.Taylor & Francis Group, London, NY, 2002.
  2. Wiesenfeld E. Aroma profiles of various Lavandula species. Noville, South Hackensack, NY, 1999.
  3. Kara N., Bayadar H. Essential oil characteristics of lavandins (Lavandula ½ intermedia Emeric ex Loisel.) of Isparta province, Kuyucak district, where lavender production center of Turkey. Journal of Selcuk Agriculture and Food Sciences, 2011, 25(4): 41-45.
  4. Guitton Y.L., Nicole F., Moja S., Valot N., Jullien F., Legendre L. Differential  accumulation  of  volatile  terpene  and terpene  synthase  mRNAs  during  lavender  (Lavandula  angustifolia and L. × intermedia) inflorescence development. Physiologia Plantarum, 2010, 138(2): 150-163 CrossRef
  5. ISO 3054:2017 Essential oil of lavandin Abrial (Lavandula angustifolia Mill. ½ Lavandula latifolia Medik.), French type. 2017. Accessed https://www.iso.org/standard/67961.html. Available June 19, 2018.
  6. Renaud E.N.C., Charles D.J., Simon J.E. Essential oil quantity and composition from 10 cultivars of organically grown Lavender and Lavandin. J. Essent. Oil Res., 2011, 13(4): 269-273 CrossRef
  7. Jianu C., Pop G., Gruia A.T., Horhat F.G. Chemical composition and antimicrobial activity of essential oils of lavender (Lavandula angustifolia) and lavandin (Lavandula × intermedia) grown in Western Romania. International Journal of Agriculture & Biology, 2013, 15(4): 772-776.
  8. Boeckelmann A. Monoterpene production and regulation in lavanders (Lavandula angustifolia × La-vandula × intermedia). Master thesis. University of British Columbia, Vancouver, 2008.
  9. Bajalan I., Rouzbahani R., Pirbalouti A.G., Maggi F. Chemical composition and antibacterial activity of Iranian Lavandula × hybrida. Chem. Biodiversity, 2017, 14(7): e1700064 CrossRef
  10. Blazekovic B., Vladimir-Knezevic S., Brantner A., Stefan M.B. Evaluation of antioxidant potential of Lavandula × intermedia Emeric ex Loisel. ‘Budrovka’: a comparative study with L. angustifolia Mill. Molecules, 2010, 15(9): 5971-5987 CrossRef
  11. Palii A.E., Rabotyagov V.D. Khimiya rastitel'nogo syr'ya, 2015, 3: 77-81 CrossRef (in Russ.).
  12. Woronuk G., Demissie Z., Rheault M., Mahmoud S. Biosynthesis and therapeutic properties of Lavandula essential oil constituents. Planta Medica, 2011, 77(1): 7-15 CrossRef
  13. Rabotyagov V.D., Akimov Yu.A. Genetika, 1986, 22(7): 1163-1172 (in Russ.).
  14. Urwin N.A.R., Horsnell J., Therese M. Generation and characterization of colchicine autotetraploid Lavandula angustifolia. Euphytica, 2007, 156(1-2): 257-266 CrossRef
  15. Urwin N.A.R. Generation and characterization of colchicine-induced polyploidy Lavandula × intermedia. Euphytica, 2014, 197(3): 331-339 CrossRef
  16. Tsuro M., Ito Y., Morisue T., Nakao Y. Induction and characterization of chromosome-doubled Lavandin (Lavandula × intermedia Emeric ex Loisel.) by colchicine treatment of leaf-derived callus. Horticultural Research (Japan), 2011, 10(3): 303-308 CrossRef
  17. Raev R.T., Jordanov R., Zheljazov V. Induced polyploidy in lavender. Acta Horticulturae, 1996, 426: 561-572 CrossRef
  18. Pausheva Z.P. Praktikum po tsitologii rastenii. Moscow, 1988 (in Russ.).
  19. Adams R.P. Identification of essential oil compounds by gas chromatography/quadrupole mass spectroscopy. Allured Pub. Corp., USA, 2007.
  20. Rabotyagov V.D., Mitrofanova O.V. Byulleten' Gosudarstvennogo Nikitskogo botanicheskogo sada, 2016, 118: 18-23 (in Russ.).
  21. Adal A.M., Demissie Z.A., Mahmoud S.S. Identification, validation and crossspecies transferability of novel Lavandula EST-SSRs. Planta, 2015, 241(4): 987-1004 CrossRef
  22. Cavanagh H.M.A., Wilkinson J.M. Lavender essential oil: a review. Australian Infection Control, 2005, 10(1): 35-37 CrossRef
  23. Carrasco A., Martinez-Gutierrez R., Tomas V., Tudela J. Lavandin (Lavandula× intermedia Emeric ex Loiseleur) essential oil from Spain: determination of aromatic profile by gas chromatography—mass spectrometry, antioxidant and lipoxygenase inhibitory bioactivities. Nat. Prod. Res., 2016, 30(10): 1123-1130 CrossRef
  24. Tsuro M., Ikedo H. Changes in morphological phenotypes and essential oil components in lavandin (Lavandula × intermedia Emeric ex Loisel.) transformed with wildtype strains of Agrobacterium rhizogenes.Scientia Horticulturae, 2011, 130(3): 647-652 CrossRef
  25. Bombarda I., Dupuy N., Le Van Da J.P., Gaydou E. Comparative chemometric analyses of geographic origins and compositions of lavandin var. Grosso essential oils by midinfrared spectroscopy and gas chromatography. Anal. Chim. Acta, 2008, 613(1): 31-39 CrossRef
  26. Rabotyagov V.D., Khokhlov Yu.S., Palii A.E. Byulleten' Gosudarstvennogo Nikitskogo botanicheskogo sada, 2017, 123: 83-89 (in Russ.). 
  27. Kivrak S. Essential oil composition and antioxidant activities of eight cultivars of Lavender and Lavandin from western Anatolia. Industrial Crops and Products, 2018, 117: 88-96 CrossRef
  28. Barocelli E., Calcina F., Chiavarini M., Impicciatorea M., Brunib R., Bianchib A., Ballabeni V. Antinociceptive and gastroprotective effects of inhaled and orally administered Lavandula hybrida Reverchon ‘Grosso’ essential oil. Life Sci., 2004, 76(2): 213-223 CrossRef

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