doi: 10.15389/agrobiology.2017.3.561eng

UDC 633.11:631.524.86


Stagonospora nodorum BLOTCH

T.M. Kolomiets, L.F. Pankratova, E.V. Pakholkova

All-Russian Research Institute of Phytopathology, Federal Agency of Scientific Organizations, 5, ul. Institute, pos. Bol’shie Vyazemy, Odintsovskii Region, Moscow Province, 143050 Russia,

Kolomiets T.M.
Pankratova L.F.

Received June 16, 2016


Septoria tritici blotch (STB) or Stagonospora nodorum blotch (SNB) are among the most harmful and economically significant diseases of wheat in the grain growing regions of the world, especially in the countries with a temperate climate. In epiphytotic years the losses from the disease can reach 30-40 %. In Russia the diseases holds a dominant position in a pathogenic complex of fungus diseases of grain crops. In this paper we first determined the parameters of partial resistance in the cultivars of wheat (genus Triticum) from the collection of the Germplasm Resources Information Network (GRIN, USA) using the stable strains of Septoria tritici and Stagonospora nodorum pathogens. The aim of our study was to select wheat varieties with long-term resistance to blotch based on field and laboratory tests. A long-term study (2009-2015) of the disease development on the wheat cultivars from GRIN Collection were conducted at artificial infection in infection nursery (Central region of Russia, Moscow Province). The samples studied belonged to various genetic groups. A total of 20 samples were diploids (2n = 14), 409 samples were tetraploids (2n = 28), 1688 samples were hexaploids (2n = 42), and also 397 lines derived from crossing of Triticum aestivum with Aegilotriticum were tested. The area under disease progress curve was determined, and the index of resistance (IR) was calculated. The cultivars, that characterized by slow development of the disease in the field, i.e. with high-and middle IR, were selected for laboratory studies. The plants were grown in artificial climate chambers till the 3rd leaf fully unfolded. Pieces of leaves were inoculated by a drop of spore suspension of S. tritici (4 isolates) or St. nodorum (4 isolates), 10 replications per each variety-to-pathotype combination. The samples were grouped according to the latent period length and size of infectious spots. As a result, 191 samples of T. aestivum subsp. aestivum and a sample of T. aestivum subsp. spelta with a high index of resistance to the disease were selected among hexaploid wheat; 16 samples were found in tetraploid wheat, including 8 samples of T. turgidum subsp. durum, 2 samples of T. turgidum subsp. turgidum, 3 samples of T. turgidum subsp. dicoccon, 3 samples of T. timopheevii subsp. timopheevii; and 4 samples were selected from diploid wheat T. monococcum subsp. aegilopoides. Eleven lines derived from crossing of T. aestivum and Aegilotriticum sp. showed the slowed-down in the disease development. The selected hexaploid wheat cultivars were mostly from North American ecology-geographical group of T. aestivum subsp. aestivum, including 77 cultivars from the USA and 18 — from Canada (34.5 % in total). Selected tetraploid wheat samples of T. turgidum subsp. durum were from North and Central America, and those of T. turgidum subsp. turgidum, T. timopheevii subsp. timopheevii and T. turgidum subsp. dicoccon from Europe and Asia. The samples from Iraq and Hungary with a high index of blotch resistance were found among diploid wheat T. monococcum subsp. aegilopoides. The synthetic lines of wheat from the USA and Mexico were also characterized by a slowed-down development of the disease. Thus the wheat cultivars with partial resistance have been revealed, including 10 cultivars with partial resistance to Septoria tritici blotch and 40 cultivars — to Stagonospora nodorum blotch. The accessions PI 494096 Tadinia, Cltr 17904 Owens, Cltr 15645 II-62-4 (USA), VIR 63915 Flame (England), Cltr 14492 Azteca, PI 520555 Alondora ‘S’ (Mexico), PI 404115 Timson (Australia), Cltr 11765 Chinese 166 (Germany), PI 422413 CNT 1 (Brazil), PI 168724 Benvenuto, Cltr 15378 Piamontes, PI 344468 Piamontes Inta (Argentina), PI 306551 2944 (Romania), PI 355706 69Z5.715 (Azerbaijan), PI 355560 SK 1B (Switzerland), PI 94743 290 (Russia) are of special interest for breeding as a source of long term resistance.

Keywords: Septoria tritici blotch, STB, Stagonospora nodorum blotch, SNB, partial resistance, long-term resistance, Triticum L., diploid, tetraploid and hexaploid wheat, synthetic lines, the Germplasm Resources Information Network (GRIN) Collection.


Full article (Rus)

Full text (Eng)



  1. Ponomarenko A., Goodwin S.B., Kema G. Septoria tritici blotch (STB). The Plant Health Instructor, 2011 CrossRef
  2. Brown J.K.M., Arraiano L.S. Association genetics of resistance to Septoria tritici blotch in north-west European wheat cultivars and breeding lines, and implications for resistance breeding. 8th International Symposium on Mycosphaerella and Stagonospora diseases of Cereals. Book of Abstracts. Mexico city, Mexico, 2011: 48.
  3. Wiik L. Control of fungal diseases in winter wheat. Evaluation of long-term field research in southern Sweden. Acta Universitatis agriculturae Sueciae, 2009, 97: 3-45.
  4. Suffert F., Sache I., Lannou C. Assessment of quantitative traits of aggressiveness in Mycosphaerella graminicola on adult wheat plants. Plant Pathol., 2013: 62: 1330-1341 CrossRef
  5. Jørgensen L.N., Hovmøller M.S., Hansen J.G., Lassen P., Clark B., Bayles R., Rodemann B., Flath K., Jahn M., Goral T., Czembor J.J., Cheyron P., Maumene C., De Pope C., Ban R., Cordsen Nielsen G., Berg G. IPM strategies and their dilemmas including an introduction to of Integrative Agriculture, 2014, 13: 265-281 CrossRef
  6. Arraiano L.S., Kirby J., Brown J.K.M. Cytogenetic analysis of the susceptibility of the wheat line Hobbit sib (Dwarf A) to Septoria tritici blotch. Theor. Appl. Genet., 2007, 116(1): 113-122 CrossRef
  7. Arrfiano L.S., Braiding P.A., Debryver F., Brown J.K.M. Resistance of wheat to septoria tritici blotch (Mycosphaerella graminicola)and associations with ideotype and the 1BL-1RS translocation. Plant Pathol., 2006, 55: 54-61 CrossRef
  8. McDonald M.C., McDonald B.A., Solomon P.S. Recent advances in the Zymoseptoria tritici—wheat interaction: insights from pathogenomics. Front. Plant Sci., 2015, 6: 102 CrossRef
  9. Raman H., Milgate A. Molecular breeding for Septoria tritici blotch resistance in wheat. Cereal Res. Commun., 2012, 40(4): 451-466 CrossRef
  10. Takele A., Lencho A., Getaneh W/Ab, Hailu E., Kassa B. Status of wheat Septoria leaf blotch (Septaria tritici Roberge in Desmaz) in South West and Western Shewa Zones of Oromiya Regional State, Ethiopia. Research in Plant Sciences, 2015, 3(3): 43-48.
  11. Simon M.R., Cordo C.A., Castillo N.S., Struik P.C., Borner A. Population structure of Mycosphaerella graminicola and location of genes for resistance to the pathogen: Recent advances in Argentina. International Journal of Agronomy, 2012, 2012: Article ID 680275, CrossRef
  12. Said A. Epidemics of Septoria tritici blotch and its development over time on bread wheat in Haddiya-Kambata Area of Southern Ethiopia. Journal of Biology, Agriculture and Healthcare, 2016, 6(1): 47-57.
  13. Singh P.K., Duveiller E., Singh R.P., Singh S., Herrera-Foessel S.A., Huerta-Espino J., Manes Y., Bonnett D., Dreisigasker S. Characterization of CIMMYT germplasm for resistance to Septoria diseases of wheat. 8th International Symposium on Mycosphaerella and Stagonospora diseases of Cereals. Book of Abstracts. Mexico city, Mexico, 2011: 55.
  14. Simon M.R., Perello A.E., Cordo C.A., Larran S., van der Putten P.E.L., Struik P.C. Association between Septoria tritici blotch, plant height, and heading date in wheat. Agron. J., 2005, 97(4): 1072-1081 CrossRef
  15. Zhang X., Haley S.D., Jin Y. Inheritance of Septoria tritici blotch resistance in winter wheat. Crop Sci., 2001, 41: 323-326 CrossRef
  16. Sanin S.S., Sanina A.A. Septorioz pshenitsy. Diagnostika, fitosanitarnye nablyudeniya, upravlenie zashchitoi rastenii [Septorialeaf blotch in wheat — indication, survey, control of plant protection]. Moscow, 2013 (in Russ.).
  17. Sanin S.S., Sanina A.A., Motovilin A.A., Pakholkova E.V., Korne-
    va L.G., Zhokhova T.P., Polyakova T.M. Zashchita i karantin rastenii, 2012, 4: 61-82 (in Russ.).
  18. Pakholkova E.V., Sal'nikova N.N., Kurkova N.A. Genetic structure of regional populations of Mycosphaerella graminicola (Septoria tritici), the septoria leaf blotch agent of wheat. Agricultural Biology, 2016, 51(5): 722-730 CrossRef
  19. Kolomiets T.M., Pankratova L.F., Skatenok O.O., Pakholkova E.V. Zashchita i karantin rastenii, 2015, 7: 44-46 (in Russ.).     
  20. Pakholkova E.V., Sal'nikova N.N., Akimova E.A., Sanina A.A. Materialy III Mezhdunarodnogo mikologicheskogo foruma «Sovremennaya mikologiya v Rossii», tom 5 [Proc. III Int. Forum «Present state of mycology in Russia»]. Moscow, 2015, V. 5: 107-108 (in Russ.).
  21. Pakholkova E.V. Zashchita i karantin rastenii, 2015, 3: 39-40 (in Russ.). 
  22. Zeleneva Yu.V. Vestnik Tambovskogo universiteta. Seriya: Estestvennye i tekhnicheskie nauki, 2008, 13(5): 333-337 (in Russ.).
  23. Sanin S.S., Nazarova L.N. Zashchita i karantin rastenii, 2010, 2: 70-80 (in Russ.).
  24. Kochorov, A.S., Sagitov A.O., Aubakirova A.T. Zashchita i karantin rastenii, 2013, 9: 44-45 (in Russ.).     
  25. Kolomiets T.M., Pakholkova E.V., Dubovaya L.P. Otbor iskhodnogo materiala dlya sozdaniya sortov pshenitsy s dlitel'noi ustoichivost'yu k septoriozu. Metodicheskie ukazaniya [Selection of donor plants for breeding wheat varieties with durable leaf blotch resistance: recommendations]. Moscow, 2017 (in Russ.).
  26. Giligan C.A. Sustainable agriculture and plant diseases: An epidemiological perspective. Philosophical Transactions of The Royal Society B Biological Sciences, 2008, 363(1492): 741-59 CrossRef
  27. Bockus W.W., De Wolf E.D., Gill B.S., Jardine D.J., Stack J.P., Bow-
    den R.L., Fritz A.K., Martin T.J. Historical durability of resistance to wheat diseases in Kansas. Plant Health Progress, 2011, August: 2 August 2011 CrossRef
  28. Johnson D.A., Wilcoxson R.D. A table of areas under disease progress curves. Texas Agric. Exp. Stn. Tech. Bull., 1981, 1337: 2-10.
  29. Makarov A.A., Strizhekozin Yu.A., Solomatin D.A., Demicheva T.A., Kukhtina A.V. V sbornike: Immunitet sel'skokhozyaistvennykh kul'tur k vozbuditelyam gribnykh boleznei [In: Crop immunity to fungal diseases]. Moscow, 1991: 105-110 (in Russ.). 
  30. Vavilov N.I. Uchenie ob immunitete rastenii k infektsionnym zabolevaniyam. Tom 1 [Doctrine of plant immunity to infectious diseases. V. 1]. Leningrad, 1967 (in Russ.).
  31. Vavilov N.I. Proiskhozhdenie i geografiya kul'turnykh rastenii [Geographic distribution of crop origins]. Leningrad, 1987 (in Russ.).