Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2017, Vol. 52, № 3, p. 535-543.
(how to cite this article)

doi: 10.15389/agrobiology.2017.3.535eng

UDC 633.11:631.527:577.21:577.088

Acknowledgements:
Supported by the grant from Russian Science Foundation, project
№ 16-16-00097 of January 27, 2016

 

PLUG MARKERS TO DETECT ALIEN GENETIC MATERIAL IN
BREAD WHEAT(Triticum aestivum L.) HYBRIDS DURING BREEDING

P.A. Sokolov1, 2, A.V. Polkhovsky1, 2, P.Yu. Kroupin1, 2, V.S. Rubets2,
V.V. Pylnev2, T.I. Khupatsariya2, G.I. Karlov1, 3, M.G. Divashuk1, 2

1K.A. Timiryazev Russian State Agrarian University—Moscow Agrarian Academy, Center for Molecular Biotechnology, 49, ul. Timiryazevskaya, Moscow, 127550 Russia,
e-mail divashuk@gmail.com (corresponding author);
2K.A. Timiryazev Russian State Agrarian University—Moscow Agrarian Academy, Department of Genetics, Biotechnology,Plant Breeding & Seed Science, 49, ul. Timiryazevskaya, Moscow, 127550 Russia, e-mail genetics@timacad.ru, selection@timacad.ru, pav2395147@yandex.ru;
3All-Russian Research Institute of Agricultural Biotechnology, Federal Agency of Scientific Organizations, 42, ul. Timiryazevskaya, Moscow, 127550 Russia,
e-mail karlovg@gmail.com

ORCID:
Sokolov P.A. orcid.org/0000-0002-9301-8175
Polkhovsky A.V. orcid.org/0000-0003-0746-1902
Kroupin P.Yu. orcid.org/0000-0001-6858-3941
Rubets V.S. orcid.org/0000-0003-1233-8837
Pylnev V.V. orcid.org/0000-0003-0400-0609
Karlov G.I. orcid.org/0000-0002-9016-103X
Divashuk M.G. orcid.org/0000-0001-6221-3659

Received October 11, 2016

 

Molecular markers make it possible to monitor the directional introgression of alien genetic material to the genome of wheat. Among the diverse molecular markers, the PLUG (PCR-based landmark unique gene) markers have the greatest advantage for analyzing distant hybrids due to the ability to amplify polymorphism fragments on closely related species and to detect simultaneously three genomes within one homoeological group in one PCR. A collection of 41 entries of different breeding stages developed in Russian State Agrarian University as a result of crossing of bread wheat (Triticum aestivum L.) and wheat T. timopheevii Zhuk. was studied. All hybrids were resistant to leaf rust and powedery mildew natural infection. Using this collection of distant hybrids, we demonstrated for the first time the possibilities of PLUG markers for identifying alien introgressions in the genome of bread wheat from T. timopheevii. In order to increase the degree of polymorphism between the entries, we additionally processed the products of amplification of PLUG markers with high-frequency restriction endonucleases TaqI and HaeIII. The entries were found to carry both single and multiple introgressions. The latter entries with introgressions in more than one homeological group are of particular interest. The heterogeneity of not only the late generations of entries, but also the entries from the nursery of the preliminary cultivar estimation, which may be associated with secondary chasmogamy, is shown. As a result of the studies, 14 PLUG markers for the long and short chromosome arm of each homeological group have been selected, which can be recommended for the detection of the genetic material of T. timopheevii in distant hybrids of T. timopheevii ½ T. aestivum. The advantages of PLUG markers as a tool for monitoring the transfer of alien hereditary material into the genome of bread wheat are discussed.

Keywords: common wheat, Triticum aestivum, Triticum timopheevii, wide hybridization, molecular markers, PLUG, PCR.

 

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