UDC 634.8:663.253.34:613.292:616.1

Supported by Russian Ministry of Education (Unique identifier PNI RFMEFI60414X0077 Agreement № 14.604.21.0077)



A.V. Kubyshkin1, A.M. Avidzba2, V.S. Borisyuk3, V.S. Stoyanov3,
I.I. Fomochkina1, Yu.A. Ogai2, I.V. Chernousova2, G.P. Zaitsev2,
T.I. Guguchkina4, V.A. Markosov4, N.M. Ageeva4, Yu.I. Shramko1

1S.I. Georgievskii Medical Academy of V.I. Vernadsky Crimean Federal University, 5/7, bul. Lenina, Simferopol, Republic of Crimea, 295051 Russia,
e-mail Kubyshkin_av@mail.ru (corresponding author), scipro@csmu.strace.net, soniashko@mail.ru, yuliana.shramko@yandex.ru;
2National Research Institute for Grape and Wine Magarach RAS, Federal Agency of Scientific Organizations, 31, ul. Kirova, Yalta, Republic of Crimea, 298671 Russia,
e-mail magarach@rambler.ru, enoant@yandex.ru, chernblack@mail.ru, gorg-83@mail.ru;
3Sanatorium Ai-Petri, 15, sh. Alupkinskoe, pgt Koreiz-1, Yalta, Republic of Crimea, 298671 Russia, e-mail ai-petry@mail.ru;
4North Caucasian Regional Research Institute of Horticulture and Viticulture, Federal Agency of Scientific Organizations, 39, ul. im. 40-letiya Pobedy, Krasnodar, 350901 Russia, e-mail guguchkina@mail.ru, ageyeva@inbox.ru

Kubyshkin A.V. orcid.org/0000-0002-1309-4005
Avidzba A.M. orcid.org/0000-0002-2354-1374
Fomochkina I.I. orcid.org/0000-0003-3065-5748
Ogai Yu.A. orcid.org/0000-0002-7619-0766
Chernousova I.V. orcid.org/0000-0001-5374-7683
Zaitsev G.P. orcid.org/0000-0001-6416-8417
Shramko Yu.I. orcid.org/0000-0003-4946-7317

Received November 9, 2016


In recent years, as cardiovascular mortality is increasing, the interest in studying the influence of polyphenol-rich grape products (PRGP) on cardiovascular risks is constantly growing. The estimation of a safe and effective dose of PRGP deserves special attention, because an excessive consumption can lead to endothelial dysfunction and oxidative stress. The hygienic and curative properties the young branches of grapevine, leaves, berries, juice and wine are used in traditional medicine for a long time. The curative properties of grapes are known to be due to the presence of biologically active grape polyphenols, which are accumulated in grapeskin, pulp, and seeds, etc. Polyphenols are extracted during alcoholic fermentation and determine the antioxidant status and biological activity of wines and other grape products. Here our objective was to analyze polyphenols in red wine (RW) and extracts from red grapes (EPG) and to compare the influence of fixed concentration of polyphenols on cardiovascular parameters, lipid peroxidation (LPO) and lipid metabolism at ischemic heart disease (IHD) and essential hypertension (EH) in the course of the SPA and resort-based treatment of 259 patients. The clinical trials of therapeutic and prophylactic properties of the experimental samples of red wine and extracts of polyphenols from red grapes showed that the use of these products as part of complex therapy contributes to the significant improvement of lipid metabolism, i.e. total cholesterol was reduced by 22 % and the atherogenic index decreased by 10 %, to a reduced free radical oxidation (end products of LPO decreased by 30.3 % when using the RW, and by 32.3 % in case of EPG), as compared to the patients from control groups who were not provided with PRGP  in addition to a standard rehabilitation. The normalization of cardiovascular indexes also occurred. In the most patients, the tolerance to physical activity increased by 22.4 % compared to the control group. The clinical effect was achieved during a 14-day course at a daily dose of 3.6 ml/kg for RW, and of 0.45 ml/kg for EPG. The mechanisms of action of these polyphenolic products and the possibility of their use for primary and secondary prevention of disorders in patients with cardiovascular disease are discussed.

Keywords: grape polyphenols, antioxidant activity, ischemic heart disease, hypertension, red wine, extract of polyphenols from red grapes.


Full article (Rus)

Full text (Eng)



  1. Montin'yak M. Sekrety zdorovogo pitaniya [Secrets of a healthy diet]. Moscow, 2006 (in Russ.).
  2. Ogai Yu.A. Vinogradarstvo i vinodelie, 2000, 1: 37-38 (in Russ.).
  3. Zagaiko A.L., Krasil'nikova O.A., Kravchenko A.B., Voloshchen-
    ko M.V., Ogai Yu.A. Biologicheski aktivnye veshchestva vinograda i zdorov'e [Bioactive substances of grape and human health benefits]. A.L. Zagaiko (ed.). Khar'kov, 2012 (in Russ.).  
  4. Haas I.C., Toaldo I.M., de Gois J.S., Borges D.L., Petkowicz C.L., Bordignon-Luiz M.T. Phytochemicals, monosaccharides and elemental composition of the non-pomace constituent of organic and conventional grape juices (Vitis labrusca L.): Effect of drying on the bioactive content. Plant Foods Hum. Nutr., 2016, 71(4): 422-528 CrossRef
  5. Ronksley P.E., Brien S.E., Turner B.J., Mukamal K.J., Ghali W.A. Association of alcohol consumption with selected cardiovascular disease outcomes: a systematic review and meta-analysis. BMJ, 2011, 342: d671 CrossRef
  6. Shevchenko O., Mishnev O., Shevchenko A., Trusov O., Slastnikova I. Ishemicheskaya bolezn' serdtsa [Cardiac ischemia]. Moscow, 2005 (in Russ.).  
  7. Badimon L., Vilahur G. LDL-cholesterol versus HDL-cholesterol in the atherosclerotic plaque: inflammatory resolution versus thrombotic chaos. Ann. NY Acad. Sci., 2012, 1254: 18-32 CrossRef
  8. Avidzba A., Kubyshkin A., Guguchkina T., Markosov V., Katsev A., Naumova N., Shramko Yu., Zaitsev G., Chernousova I., Ogai Yu., Fomochkina I. Voprosy pitaniya, 2016, 85(1): 99-110 (in Russ.).
  9. Xiang L., Xiao L., Wang Y., Li H., Huang Z., He X. Health benefits of wine: don't expect resveratrol too much. Food Chem., 2014, 156: 258-263 CrossRef
  10. Piepoli M.F., Hoes A.W., Agewall S., Albus C., Brotons C., Catapano A.L. et al. European Guidelines on cardiovascular disease prevention in clinical practice: the Sixth Joint Task Force of the European Society of Cardiology and Other Societies on Cardiovascular Disease Prevention in Clinical Practice (constituted by representatives of 10 societies and by invited experts): developed with the special contribution of the European Association for Cardiovascular Prevention & Rehabilitation (EACPR). Eur. Heart J., 2016 37(29): 2315-2381 CrossRef
  11. Mori T.A., Burke V., Beilin L.J., Puddey I.B. Randomized controlled intervention of the effects of alcohol on blood pressure in premenopausal women. Hypertension, 2015; 66(3): 517-523 CrossRef
  12. Mukamal K. Alcohol intake and noncoronary cardiovascular diseases. Ann. Epidemiol., 2007, 17(5): S8-S12 CrossRef
  13. Wu D., Cederbaum A.I. Alcohol, oxidative stress, and free radical damage. Alcohol Res. Health, 2003, 27(4): 277-284 (PMID: 15540798).
  14. Quideau S., Deffieux D., Douat-Casassus C., Pouységu L. Plant polyphenols: chemical properties, biological activities, and synthesis. Angew. Chem. Int. Ed., 2011, 50(3): 586-621 CrossRef
  15. Garaguso I., Nardini M. Polyphenols content, phenolics profile and antioxidant activity of organic red wines produced without sulfur dioxide/sulfites addition in comparison to conventional red wines. Food Chem., 2015, 179: 336-342 CrossRef
  16. Ogai Yu.A., Alekseeva L.M., Sikazan O.M., Katrich L.I. Materialy konferentsii «Biologicheski aktivnye prirodnye soedineniya vinograda: gigienicheskie i meditsinskie effekty primeneniya produktov s vysokim soderzhaniem polifenolov vinograda» [Proc. Conf. «Grape natural bioactive substances of grape: sanitary and curative effects of high-polyphenol-based products»]. Simferopol', 2005, V. 41, Part 1: 14-20 (in Russ.).  
  17. Apostolidou C., Adamopoulos K., Lymperaki E., Iliadis S., Papapreponis P., Kourtidou-Papadeli C. Cardiovascular risk and benefits from antioxidant dietary intervention with red wine in asymptomatic hypercholesterolemics. Clin. Nutr. ESPEN, 2015, 10(6): e224-e233 CrossRef
  18. Gepner Y., Golan R., Harman-Boehm I., Iris Shai L. Effects of initiating moderate alcohol intake on cardiometabolic risk in adults with type 2 diabetes: a 2-year randomized, controlled trial. Ann. Intern. Med., 2015, 163(8): 569-579 CrossRef
  19. Wang R., Sun Q., Chang Q. Soil types effect on grape and wine composition in Helan Mountain area of Ningxia. PLoS ONE, 2015, 10(2): e0116690 CrossRef
  20. Mulero J., Martínez G., Oliva J., Cermeño S., Cayuela J.M., Zafrilla P., Martínez-Cachá A., Barba A. Phenolic compounds and antioxidant activity of red wine made from grapes treated with different fungicides. Food Chem., 2015, 180: 25-31 CrossRef
  21. R.4.1.1672-03 Rukovodstvo po metodam kontrolya kachestva i bezopasnosti biologicheski aktivnykh dobavok k pishche [Quality and safety control of bioactive food additives — guidelines. R.4.1.1672-03]. Moscow, 2003 (in Russ.).
  22. GOST R 54037-2010 Produkty pishchevye. Opredelenie soderzhaniya vodorastvorimykh antioksidantov amperometricheskim metodom v ovoshchakh, fruktakh, produktakh ikh pererabotki, alkogol'nykh i bezalkogol'nykh napitkakh [RF State Standard R 54037-2010 Foodstuffs. Amperometric estimation of water-soluble antioxidants in vegetable and fruit products, non-alcoholic and alcoholic drinks].Moscow, 2010 (in Russ.).
  23. Highton J., Hessian P. A solid-phase enzyme immunoassay for C-reactive protein: clinical value and the effect of rheumatoid factor. J. Immunol. Methods, 1984, 68: 185-192.
  24. Vladimirov Yu.A., Archakov A.I. Perekisnoe okislenie lipidov v biologicheskikh membranakh [Lipid peroxidation in biomembranes]. Moscow, 1972 (in Russ.).