doi: 10.15389/agrobiology.2016.3.318eng

UDC 635.64:632.12:581.143:58.086

Supported by Russian Foundation for Basic Research, project № 16-34-01331 (mol_a)



L.R. Bogoutdinova1, 2, G.B. Baranova1, E.N. Baranova1,
M.R. Khaliluev1, 2

1All-Russian Research Institute of Agricultural Biotechnology, Federal Agency of Scientific Organizations, 42, ul. Timiryazevskaya, Moscow, 127550 Russia, e-mail;
2K.A. Timiryazev Russian State Agrarian University—Moscow Agrarian Academy, 49, ul. Timiryazevskaya, Moscow, 127550 Russia

Received January 9, 2016


Improving plant resistance to unfavorable environmental factors is one of the important tasks of modern agricultural production. The cultivated tomato is highly sensitive to salinity. Understanding the physiological, biochemical, molecular and genetic adaptive mechanisms of tomato resistance to salinity as a complex trait is an essential part of most fundamental studies, the results of which are increasingly finding practical application during the last decade. However, the data on the effect of sodium chloride on cell organization of different tomato tissues and organs are scarce. Thus, the purpose of the study was to investigate cell organization of the epidermis and parenchyma cortical tissues of tomato hypocotyl (Solanum lycopersicum L. line YaLF and cultivar Rekordsmen) under chloride salinity in vitro. Fragments of 10-12-day-old aseptically germinated tomato seedlings, whose roots have been removed, were transferred on root induction medium (1/2 МS, 2 % sucrose, 0.2 mg/l indole-3-butyric acid) supplemented with 0-250 mМ NaCl. After 8 days in culture, middle part of hypocotyls were excised from rooted seedlings and prepared for light microscopy. Histological examination revealed significant differences between genotypes in shape and average cross-sectional areas of the epidermal and cortical parenchyma cells of hypocotyl. The addition of Na+ and Cl- ions to culture medium significantly affected the size of the intercellular spaces in the cortical parenchyma as well as the average cross-sectional areas and shape epidermal and cortical parenchyma hypocotyl cells of both tomato genotypes. The average cross-sectional areas of epidermal and cortical parenchyma hypocotyl cells of tomato line YaLF under 50 mM NaCl were significantly less (1,2 and 1,6 times, respectively) compared with control conditions (medium without NaCl). Epidermal and cortical parenchyma hypocotyl cells of tomato cultivar Rekordsmen decreased in size at higher concentrations of NaCl in the culture medium (100 and 150 mM NaCl, respectively). Dramatic increase in the cells areas of both tissue types of tomato line YaLF were observed under 250 mM NaCl salinity. In addition, under high salinity treatments there was a considerable change in the shape of epidermal (cells obtained angular contours) and cortical parenchyma hypocotyl cells (cell flattening) of line YaLF. Unlike line YaLF, the cross-sectional areas of epidermal and cortical parenchyma hypocotyl cells of tomato cultivar Rekordsmen was no statistically significant differences between 0 and 250 mM NaCl treatment. The dramatic difference between the two tomato genotypes was observed by a change in the cross-sectional areas of intercellular spaces in cortical parenchyma hypocotyl cells under salt treatments. Epidermal and cortical parenchyma cells of tomato hypocotyls cultivar Rekordsmen were less sensitive to the presence of NaCl in the culture medium, compared with the line YaLF. The revealed changes in shape and size of epidermal and cortical parenchyma hypocotyl cells can be used as cytological markers for comparative evaluation of tomato genotypes in sensitivity and/or resistance to salinity.

Keywords: tomato, Solanum lycopersicum L., salt stress, hypocotyl anatomy, in vitroculture.


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  1. Boyer J.S. Plant productivity and environment. Science, 1982, 218: 443-448 CrossRef
  2. Foolad M.R. Recent advances in genetics of salt tolerance in tomato. Plant Cell Tiss. Org. Cult., 2004, 76(2): 101-119 CrossRef
  3. Flowers T.J. Improving crop salt tolerance. J. Exp. Bot., 2004, 55(396): 307-319 CrossRef
  4. Chinnusamy V., Jagendorf A., Zhu J.-K. Understanding and improving salt tolerance in plants. Crop Sci., 2005, 45(2): 437-448 CrossRef
  5. Cuartero J., Bolarin M.C., Asins M.J. Moreno V. Increasing salt tolerance in the tomato. J. Exp. Bot., 2006, 57(5): 1045-1058 CrossRef
  6. Shibli R.A., Kushad M., Yousef G.G., Lila M.A. Physiological and biochemical responses of tomato microshoots to induced salinity stress with associated ethylene accumulation. Plant Growth Regulation, 2007, 51(2): 159-169 CrossRef
  7. Perez-Alfocea F., Estañ M.T., Caro M., Bolarín M.C. Responses of tomato cultivars to salinity. Plant and Soil, 1993, 150(2): 203-211 CrossRef
  8. Cano E.A., Perez-Alfocea F., Moreno V., Caro M., Bolarín M.C. Evaluation of salt tolerance in cultivated and wild tomato species through in vitro shoot apex culture. Plant Cell Tiss. Org. Cult., 1998, 53(1): 19-26 CrossRef
  9. Baragé M. Identificación de fuentes de tolerancia a la salinidad y al estrés hídrico en especies silvestres de la familia Cucurbitaceae. PhD thesis. Universidad Politecnica de Valencia, 2002.
  10. Baranova E.N., Akanov E.N., Gulevich A.A., Kurenina L.V., Danilova S.A., Khaliluev M.R. Doklady RASKHN, 2013, 6: 13-16 (in Russ.).
  11. Romero-Aranda R., Soria T., Cuartero S. Tomato plant-water uptake and plant-water relationships under saline growth conditions. Plant Sci., 2001, 160(2): 265-272 CrossRef
  12. Yadav S., Irfan M., Ahmad A., Hayat S. Causes of salinity and plant manifestations to salt stress: a review. J. Environ. Biol., 2011, 32(5): 667-685.
  13. Munns R., Tester M. Mechanisms of salinity tolerance. Annu. Rev. Plant Biol., 2008, 59: 651-681 CrossRef
  14. Baranova E.N., Gulevich A.A. Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2006, 1: 39-56 (in Russ.).
  15. Foolad M.R. Genome mapping and molecular breeding of tomato. Int. J. Plant Genomics, 2007: 64358 CrossRef
  16. Monforte A.J., Asíns M.J., Carbonell E.A. Salt tolerance in Lycopersicon species. IV. Efficiency of marker-assisted selection for salt tolerance improvement. Theor. Appl. Genet., 1996, 93(5): 765-772 CrossRef
  17. Bhatnagar-Mathur M., Vadez Z., Sharma K.K. Transgenic approaches for abiotic stress tolerance in plants: retrospect and prospects. Plant Cell Rep., 2008, 27(3): 411-424 CrossRef
  18. Al-Tardeh S., Iraki N. Morphological and anatomical responses of two Palestinian tomato (Solanum lycopersicon L.) cultivars to salinity during seed germination and early growth stages. Afr. J. Biotechnol., 2013, 12(30): 4788-4797 CrossRef
  19. Sam O., Ramírez C., Coronado M.J., Testillano P.S., Risueño M.C. Changes in tomato leaves induced by NaCl stress: leaf organization and cell ultrastructure. Biologia Plantarum, 2004, 47(3): 361-366 CrossRef
  20. Strogonov B.P., Kabanov V.V., Shevyakova N.I., Lapina L.P., Komizerko E.I., Popov B.A., Dostanova R.Kh., Prikhod'ko L.S., Kursanov A.L. Struktura i funktsii kletok rastenii pri zasolenii [Plant cell structure and functions under salinity]. Moscow, 1970 (in Russ.).
  21. Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, 1962, 15: 473-497 CrossRef
  22. Uikli B. Elektronnaya mikroskopiya dlya nachinayushchikh [Electron microscopy for beginners]. Moscow, 1975 (in Russ.).
  23. Gorshkova T.A. Rastitel'naya kletochnaya stenka kak dinamichnaya sistema [Plant cell wall as a dynamic system]. Moscow, 2007 (in Russ.).
  24. Kudoyarova G.R., Kholodova V.P., Veselov D.S. Fiziologiya rastenii, 2013, 60(2): 155-165 (in Russ.) CrossRef
  25. Kholodova V.P., Meshcheryakov A.B., Aleksandrova S.N., Kuznetsov Vl.V. Vestnik Nizhegorodskogo gosudarstvennogo universiteta im. N.I. Lobachevskogo. Seriya biologiya, 2001, 1: 151-154 (in Russ.).
  26. Wang C., Zhang L., Yuan M., Ge Y., Liu Y., Fan J., Ruan Y., Cui Z., Tong S., Zhang S. The microfilament cytoskeleton plays a vital role in salt and osmotic stress tolerance in Arabidopsis. Plant Biology, 2010, 12: 70-98 CrossRef