Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2015, Vol. 50, № 3, pp. 377-383.

doi: 10.15389/agrobiology.2015.3.377eng

UDC 633.853.52:579.64(470.319)

EFFICIENCY OF INTRODUCTION OF ACC-UTILIZING RHIZOBACTERIA IN SOYBEAN AGROCENOSES IN THE OREL REGION

Yu.V. Kuzmicheva, I.L. Tychinskaya, S.N. Petrova, N.V. Parakhin

Orel State Agrarian University, 69, ul. Generala Rodina, Orel, 302019 Russia,
e-mail juliemons@yandex.ru;

Received March 30, 2015

 

Resource-saving technologies contribute to the reproduction of the soil fertility and increase sustainability of agrocenoses. Legumes are most relevant to the modern requirements for formation of ecological balance. They form a multicomponent symbiosis with various groups of useful soil microflora, which not only improves soil properties and increase the yield and its quality, but also makes them a valuable precursor in the rotation. In this regard, promising search and practical use of new microorganisms with multifunctional properties that will improve the economic efficiency of legume cropcultivation. Of particular interest is the use of ACC-utilizing bacteria having a versatile anti-stress effect on plants.We have carried out a study on the impact of the introduction of promising strains ACC utilizing rhizobacteria (Pseudomonas oryzihabitans Ep4 and Variovorax paradoxus 3P-4) and nodule bacteria (Bradyrhizobium japonicum 634b) on efficiency of cultivation of the northern soybean ecotype Svapa in the Orel region depending on mineral nutrition (NPK 100 % and NPK 70 %). The strain of nodule bacteria was used for inoculation of seeds in the amount of 200 g per hectare seed rate. Strains of associative ACC utilizing microorganisms were added to the rows in the seedling stage (10 % solution). NPK was applied to the soil before planting in the form of fertilizer mixture with the percentage of mineral nutrients 10:26:26. Biochemical assessment of the quality of soybean was performed in the laboratory conditions. In forming symbioses with introduced microflora the soybean agrocenoses were more environmentally adaptable, which was reflected in increasing the productivity in drought years by 35-98 %. The positive effect of the bacteria was evident in increasing the grain protein content by 34 %. Getting a high yield and high yield quality of the studied soybean variety due to formation of effective plant-microbe system leaded to the increase of total protein up to 1.05-1.30 t/ha. The introduction of microorganisms was the most effective at lowering the dose of mineral fertilizers by 30 %. It was found that the use of rhizobacteria Pseudomonas oryzihabitans together with nodule bacteria ensured maximum economic benefit and allowed to obtain cheaper and high-quality protein with high profitability. This will reduce the costs associated with the application of mineral fertilizers without crop losses.

Keywords: soybean, agrocenosis, ACC-utilizing rhizobacteria, symbiosis, economic efficiency, resource conservation.

 

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REFERENCES

  1. Zhuchenko A.A. Vestnik OrelGAU, 2009, 18(3): 8-12.
  2. Sytnikov D.M. Bіotekhnologіya, 2012, 5(4): 34-45.
  3. Davydenko O.G., Golenko D.V. Soya dlya umerennogo klimata [Soybean production under temperate climate conditions]. Minsk, 2004.
  4. Parakhin N.V., Petrova S.N. Sel'skokhozyaistvennye aspekty simbioticheskoi azotfiksatsii [Agricultural aspects of symbiotic nitrogen fixation]. Moscow, 2006.
  5. Zotikov V.I., Naumkina T.S. Vestnik OrelGAU, 2007, 3: 11-14.
  6. Vance C.P., Spaink H.P., Kondorosi A., Hooykaas P.J.J. Legume symbiotic nitrogen fixation: agronomic aspects. In: The Rhizobiaceae. Dordrecht, 1998: 509-530.
  7. Kuzmicheva Yu.V., Petrova S.N. Zernobobovye i krupyanye kul'tury, 2013, 8(4): 43-48.
  8. Tikhonovich I.A., Provorov N.A. Simbiozy rastenii i mikroorganizmov: molekulyarnaya genetika agrosistem budushchego [Plant-microbe symbioses: molecular genetics of future agrosystems]. St. Petersburg, 2009.
  9. Borisov A.Yu., Shtark O.Yu., Kazakov A.E., Naumkina T.S., Chebotar' V.K. Materialy Vserossiiskoi nauchno-prakticheskoi konferentsii «Puti povysheniya ustoichivosti sel'skokhozyaistvennogo proizvodstva v sovremennykh usloviyakh» [Proc. All-Russian Conf. «Ways to improve the sustainability of agricultural production in modern conditions»]. Orel, 2005: 325-348.
  10. Kuzmicheva Yu.V., Parakhin N.V. Rossiiskaya sel'skokhozyaistvennaya nauka, 2015, 1-2: 31-33.
  11. Kuzmicheva Yu.V., Parakhin N.V. Vestnik OrelGAU, 2014, 49(4): 3-6.
  12. Parakhin N.V., Kuzmicheva Yu.V., Petrova S.N., Botuz N.I., Tychin-
    skaya I.L. VestnikOrelGAU, 2014, 47(2): 3-6.
  13. Parakhin N.V., Kuzmicheva Yu.V. Izvestiya Orenburgsko gogosudarstvennogo agrarnogouniversiteta, 2012, 38(6): 66-68.
  14. Parakhin N.V., Petrova S.N., Kuzmicheva Yu.V. Izvestiya Nizhnevolzhskogo agrouniversitetskogo kompleksa, 2012, 28(4): 85-89.
  15. Belimov A.A., Safronova V.I. Sel'skokhozyaistvennaya biologiya [Agricultural Biology], 2011, 3: 23-28 (http://www.agrobiology.ru/3-2011safronova-eng.html).
  16. Belimov A.A., Hontzeas N., Safronova V.I., Demchinskaya S.V., Piluzza G., Bullitta S., Glick B.R. Cadmium-tolerant plant growth-promoting bacteria associated with the roots of Indian mustard (Brassica juncea L. Czern.). Soil Biol. Biochem, 2005, 37: 241-250 CrossRef
  17. Czarny J.C., Grichko V.P., Glick B.R. Genetic modulation of ethylene biosynthesis and signaling in plants. Biotech. Adv., 2006, 24: 410-419 CrossRef
  18. Belimov A.A. Vzaimodeistvie assotsiativnykh bakterii i rastenii v zavisimosti ot bioticheskikh i abioticheskikh faktorov. Avtoreferat doktorskoi dissertatsii [An interaction of associative bacteria and plants depending on biotic and abiotic factors. DSci Thesis]. St. Petersburg, 2008.
  19. Cheng Z., Park E., Glick B.R. 1-Aminocyclopropane-1-carboxylate deaminase from Pseudomonas putida UW4 facilitates the growth of canola in the presence of salt. Canadian. J. Microbiol., 2007, 53: 912-918.
  20. Glick B.R. Phytoremediation: synergistic use of plants and bacteria to clean up the environment. Biotech. Adv., 2003, 21: 383-393 CrossRef
  21. Burd G.I., Dixon D.G., Glick B.R. A plant growth promoting bacterium that decreases nickel toxicity in seedlings. Appl. Environ. Microbiol., 1998, 64: 3663-3668.
  22. Holguin G., Glick B.R. Transformation of Azospirillum brasilense Cd with an ACC deaminase gene from Enterobacter cloacae UW4 fused to the Tetr gene promoter improves its fitness and plant growth promoting ability. Microbial Ecology, 2003, 4: 122-133.
  23. Belimov A.A., Dodd I.C., Safronova V.I., Davies W.J. ACC deaminase-con-taining rhizobacteria improve vegetative development and yield of potato plants grown under water-limited conditions. Aspects of Applied Biology, 2009, 98: 163-169.
  24. Saravanakumar D., Samiyappan R. ACC deaminase from Pseudomonas fluorescens mediated saline in groundnut (Arachis hypogea) plants. J. Appl. Microbiol., 2007, 102: 1283-1292 CrossRef
  25. Grichko V.P., Glick B.R. Amelioration of flooding stress by ACC deaminase-containing plant growth-promoting bacteria. Plant Physiol. Biochem., 2001, 39: 11-17.
  26. Mayak S., Tirosh T., Glick B.R. Plant growth-promotion bacteria that confer resistance to water stress in tomatoes and peppers. Plant Sci., 2004, 166: 525-530.
  27. Safronova V.I., Stepanok V.V., Engqvist G.L., Alekseyev Y.V., Belimov A.A. Root-associated bacteria containing 1-aminocyclopropane-1-carboxylate deaminase improve growth and nutrient uptake by pea genotypes cultivated in cadmium supplemented soil. Biol. Fertil. Soils, 2006, 42: 267-272 CrossRef
  28. Belimov A.A., Dodd I.C., Hontzeas N., Theobald J.C., Safronova V.I., Davies W.J. Rhizosphere bacteria containing ACC deaminase increase yield of plants grown in drying soil via both local and systemic hormone signalling. New Phytologist, 2009, 181: 413-423 CrossRef
  29. Guinel F.C., Geil R.D. A model for the development of the rhizobial and arbuscular mycorrhizal symbioses in legumes and its use to understand the roles of ethylene in the establishment of these two symbioses. Can. J. Bot., 2002, 80: 695-720 CrossRef
  30. Metodika gosudarstvennogo sortoispytaniya sel'skokhozyaistvennykh kul'tur. Obshchaya chast' [Methods of state crop variety trials. Issue 1]. Moscow, 1971. Vypusk 1.

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