Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2015, Vol. 50, № 3, pp. 361-368.

doi: 10.15389/agrobiology.2015.3.361eng

UDC 632.937:579.64:579.26

Supported by the Ministry of Education and Sciences of the Russian Federation (Agreement № 14.604.21.0024, RFMEFI60414X0024).

EFFICIENCY OF BATSIKOL BASED ON A NEW STRAIN
Bacillus thuringiensis var. darmstadiensis № 25 FOR BIOCONTROL
OF PHYTOPHAGOUS PESTS AND PHYTOPATHOGENS

S.D. Grishechkina, V.P. Ermolova

All-Russian Research Institute for Agricultural Microbiology, Federal Agency of Scientific Organizations, 3, sh. Podbel’skogo, St. Petersburg, 196608 Russia,
e-mail: svetagrishechkina@mail.ru

Received March 30, 2015

 

Environmentally friendly products are one of the most important tasks in agriculture. Microorganisms of different origin are used for the production of biological preparations pathogenic to pests. The preparations based on entomopathogenic bacteria Bacillus thuringiensis (Bt) are of the most interest due to their selective action, safety for humans, warm-blooded animals, beneficial insects and the environment. The departmental collection of beneficial microorganisms for agricultural purposes (RCAM collection, St. Petersburg) stores B. thuringiensis with known cultural, morphological, physiological, biochemical, technological, insecticidal and other properties. One of the priorities of collection strains is B. thuringiensis var. darmstadiensis (BtH10) № 25, which bases the preparation Batsikol and has a specific effect on Coleoptera and antifungal activity against a number of pathogenic fungi. Strain BtH10 № 25 deposited in the RCAM collection under registration number 01490 RCAM (patent № 2514023, 2014). To obtain qualitative preparation with a maximum accumulation of exotoxin and high titer we need to create optimal conditions for cultivation and improve the formulation of nutrient media for microorganisms-producers of biological preparations. The objective of our research was to choose nutrient medium, work out cultivation regimes (aeration, temperature, pH, dosage, form, and age of inoculum) of strain BtH10 № 25, and to determine its technological effectiveness, insecticidal and antifungal activity as well as the extent of exotoxin formation in test objects. The best results were obtained in the pea flour medium, instead of soy flour: spores titer in liquid culture was 4×109/ml, activity to housefly (exotoxin content) was LC50 3.1 mkl/g feed, the inhibitory activity was 70.0 % to Fusarium oxysporum and 75.0 % to Botrytis cinerea. It was found that barley, oat flour, and mixtures of soybean and barley flour could be used for the preparation production. The optimal development of the culture was observed at a ratio of medium volume-to-air from 1:30 to 1:15, and pH of the nutrient medium 6.65-7.20. Liquid culture media and the spore culture rinse can be used as an inoculum. Laboratory and field evaluation of preparation revealed its high efficiency against phytophagous pests (79-100 %) and plant pathogens(70-90 %).Thus, the activity of the strain B. thuringiensis var. darmstadiensis № 25 against the larvae of beetles on different crops is 1.3-1.4 times higher than the activity of strain B. thuringiensis var. darmstadiensis № 109.

Keywords: Bacillus thuringiensis, strain, batcikol, nutrient solutions, efficiency.

 

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