doi: 10.15389/agrobiology.2013.3.28eng
UDC 635.11:575.2:577.29:57.088.1
RAPD ANALYSIS OF COLLECTION ACCESSIONS OF THE GENUS Beta L. SPECIES
Yu.V. Chesnokov, V.I. Burenin, A.A. Ivanov
N.I. Vavilov Research Institute of Plant Industry, Russian Academy of Agricultural Sciences,
44, ul. Bolshaya Morskaya, St. Petersburg, 190000 Russia,
e-mail: yu.chesnokov@vir.nw.ru
Received April 11, 2012
The primitive (transient) forms and wild varieties of beet are related to cultivated beet (sugar, table, fodder and mangold), have the useful properties, can be crossed with cultivated beet and used in breeding programs. However, there are some difficulties in estimating the status of beet samples and procedure for their storage in gene banks, which are caused by problems in beet taxonomy and systematics. In particular, a correct discrimination ofВeta maritima vs В. vulgaris is hard to accomplish, and also the distinctions betweenВ. maritimа and В. adanensis or В. macrocarpa aredifficult to identify. With the use of classical morphobiological analysis and molecular RAPD-markers, the authors for the first time determined the phylogenetic relationships between different specimens of wild, primitive and cultivated Beta L. from world collection of the N.I. Vavilov All-Russian Scientific Research Institute of Plant Growing (VIR). On the basis of electrophoresis data, by means of UPGMA method the tested samples were clustered and phylogenetic trees created. The obtained data suggest the phylogenetic division of Beta L. genus, which have been proposed by V.I. Burenin in 1983, and indicate an accuracy of this classification.
Keywords: species of Beta L. genus, RAPD analysis, phylogenetic interactions.
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