doi: 10.15389/agrobiology.2013.3.28eng

UDC 635.11:575.2:577.29:57.088.1


Yu.V. Chesnokov, V.I. Burenin, A.A. Ivanov

N.I. Vavilov Research Institute of Plant Industry, Russian Academy of Agricultural Sciences,
44, ul. Bolshaya Morskaya, St. Petersburg, 190000 Russia,

Received April 11, 2012

The primitive (transient) forms and wild varieties of beet are related to cultivated beet (sugar, table, fodder and mangold), have the useful properties, can be crossed with cultivated beet and used in breeding programs. However, there are some difficulties in estimating the status of beet samples and procedure for their storage in gene banks, which are caused by problems in beet taxonomy and systematics. In particular, a correct discrimination ofВeta maritima vs В. vulgaris is hard to accomplish, and also the distinctions betweenВ. maritimа and В. adanensis or В. macrocarpa aredifficult to identify. With the use of classical morphobiological analysis and molecular RAPD-markers, the authors for the first time determined the phylogenetic relationships between different specimens of wild, primitive and cultivated Beta L. from world collection of the N.I. Vavilov All-Russian Scientific Research Institute of Plant Growing (VIR). On the basis of  electrophoresis data, by means of UPGMA method the tested samples were clustered and phylogenetic trees created. The obtained data suggest the phylogenetic division of Beta L. genus, which have been proposed by V.I. Burenin in 1983, and indicate an accuracy of this classification.

Keywords: species of Beta L. genus, RAPD analysis, phylogenetic interactions.


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1. Vavilov N.I. Teoreticheskie osnovy selektsii [Theoretical Fundamentals of Breeding]. Moscow, 1935.
2. Krasochkin V.T. Svekla. Kul'turnaya flora SSSR [Beet. Cultural Flora of the USSR]. Leningrad, 1971: 7-266.
3. Burenin V.I. Geneticheskie resursy roda Beta L. (svekla) [Genetic Resources of the Genus Beta L. (Beet)]. St. Petersburg, 2007.
4. Van Geyt J.P.C., Lange W., Oleo M., De Bock T.S.M. Natural variation within the genus Beta and its possible use for breeding sugar beet. Euphytica, 1990, 49: 57-76.
5. Vavilov N.I. Zakon gomologicheskikh ryadov v nasledstvennoi izmenchivosti [The Law of Homological Series in Hereditary Variability]. Saratov, 1920.
6. Lewellen R.T. Pre-breeding sugar beet for virus and Rhizomania resistance (abstr.). A Report on the Third Int. Beta Genetic Resources Workshop and World Network Conference. Fargo, USA, 1993: 11.
7. Teurer J.C. Pre-breeding to change root architecture (abstr.). A Report on the Third Intrl. Beta Genetic Resources Workshop and World Network Conference. Fargo, USA, 1993: 11.
8. Lange W., De Bock T.M.S., Lankhorst R.K. Pre-breeding for nematode resistance in beet (abstr.). A Report on the Third Intrl. Beta Genetic Resources Workshop and World Network Conference. Fargo, USA, 1993: 13.
9. Dalke L., Szota M. Possibility for utilizing the male sterility from Beta maritima in sugar beet breeding (abstr.). A Report on the Third Intrl. Beta Genetic Resources Workshop and World Network Conference. Fargo, USA, 1993: 14.
10. Burenin V.I., Pivovarov V.F. Svekla [Beet]. St. Petersburg, 1998.
11. Williams J.G.K., Kubilek A.R., Livak K.J., Rafalski J.A., Tingey S. DNA polymorphism ampliphied by arbitrary primers are useful as genetic markers. Nucl. Acid Res., 1990, 18: 6531-6535.
12. Chesnokov Yu.V. Geneticheskie resursy rastenii i sovremennye metody DNK-tipi-rovaniya [Genetic Resources of Plants and Modern Methods of DNA-Fingerprinting]. St. Petersburg, 2007.
13. Chesnokov Yu.V. Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2005, 1: 20-40.
14. Potokina E.K., Chesnokov Yu.V. Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2005, 3: 3-18.
15. Barzen E., Mechelke W., Ritter E., Shulte-Kappert E. An extended map of the sugar beet genome containing RFLP and RAPD loci. Theor. Appl. Genet., 1995, 90: 189-193. CrossRef
16. Nilsson N.-O., Hallden C., Hansen M., Hjerdin A., Säll T. Comparing the distribution of RAPD and RFLP markers in high density linkage map of sugar beet. Genome, 1997, 40: 644-651. CrossRef
17. Abe J., Yoshikawa H., Tsuda C. Genetic analysis for annularity and self fertility in wild relatives of sugar beet, made by using the pigment gene and some enzyme loci as markers. Proceeding of the Sugar Beet Reseach Association (Japan), 1985, No. 27.
18. Abe J., Tsuda C. Genetic analysis for enzyme variation in the section Vulgares, genus Beta. Japan. J. Breed., 1987, 37: 253-261.
19. Sabir A., Newbury H.J., Todd G., Catty J.P., Ford-Lloyd B.V. Determination of genetic stability using isozymes and RFLPs in beets plants regenerated in vitro. Theor. Appl. Genet., 1992, 84: 113-117. CrossRef
20. Mita G., Dani M., Casciari P., Pasquqli A., Selva E., Minganti C., Piccardi P. Assessment of the degree of genetic variation in beet based on RFLP analysis and taxonomy of Beta. Euphytica, 1991, 55: 1-6. CrossRef
21. Nagamine T., Catty J.P., Ford-Lloyd B.V. Phenotypic polymorphism and allele differentiation of isozymes in fodder beet, multigerm sugar beet and monogerm sugar beet. Theor. Appl. Genet., 1989, 77: 711-720. CrossRef
22. Levall M.W., Bengtsson K., Nilsson N., Hjerdin A., Hallden C. Molecular characterization of UV-treated beet somaclones using RFLP markers. Physiologia Plantarum, 1994, 90: 216-220. CrossRef
23. Boundry P., Weiber R., Saumitou-Laprade P., Pillen K., Van Dijk H., Jung C. Identification of RFLP markers closely linked to the bolting gene B and their significance for the study of the annual habit in beets (Beta vulgaris L.). Theor. Appl. Genet., 1994, 88: 852-858. CrossRef
24. Salentijin E.M.J., Sandal N.N., Klein-Lankhorst R., Lange W., De Bock T.H., Marcker K.A., Stiekema W.J. Long-range organization of the satellite DNA family flanking the beet cyst nematode resistance locus (HsI) on chromosome 1 of B. patellaris and procumbens. Theor. Appl. Genet., 1994, 89: 459-466. CrossRef
25. Lorenz M., Weihe A., Borner T. DNA fragments of organellar origin in random amplified polymorphic DNA (RAPD) patterns of sugar beet (Beta vulgaris L.). Theor. Appl. Genet., 1994, 88: 775-779. CrossRef
26. Senda M., Onodera Y., Kinoshita T., Mikami T. Mitochondrial gene variation and the relationships in the genus Beta. Theor. Appl. Genet., 1995, 90: 914-919. CrossRef
27. Dorokhov D.B., Kloke E. Genetika, 1997, 33(4): 358-365.
28. Virk P.S., Ford-Lloyd B.V., Jackson M.T., Newbury H.J. Use of RAPD for the study of diversity within plant germplasm collections. Heredity, 1995, 74: 170-179. CrossRef
29. Nei M., Li W.H. Mathematical model for studying genetic variation in terms of restriction endonuclease. PNAS USA, 1979, 76: 5269-5273.
30. Hennink S., Zeven A.C. The interpretation of Nei and Shannon-Weaver index within population variation indexes. Euphytica, 1990, 51: 235-240.
31. Sneath P.H.A., Sokal R.R. Numerical taxonomy. Freeman, San Francisco, 1973.
32. Rohlf F.J. NTSYS-pc numerical taxonomy and multivariate analysis system. Exeter, NY, 1992.
33. Shen Y., Ford-Lloyd B.V., Newbury H.J. Genetic relationships within the genus Beta determined using both PCR-based marker and DNA sequencing techniques. Heredity, 1998, 80: 624-632. CrossRef
34. Grimmer M.K., Trybush S., Hanley S., Francis S.A., Karp A., Asher M.J.C. An anchored linkage map for sugar beet based on AFLP, SNP and RAPD markers and QTL mapping of a new source of resistance to Beet necrotic yellow vein virus. Theor. Appl. Genet., 2007, 114: 1151-1160. CrossRef
35. Scholten O.E., Klein-Lankhorst R.M., Esselink D.G., De Bock T.S.M.,
Lange W. Identification and mapping of random polymorphic DNA (RAPD) markers linked to resistance against beet necrotic yellow vein virus (BNYVV) in Beta accessions. Theor. Appl. Genet., 1997, 94: 123-130. CrossRef
36. Hagihara E., Itchoda N., Habu Y., Iida S., Mikami T., Kubo T. Molecular mapping of a fertility restorer gene for Owen cytoplasmic male sterility in sugar beet. Theor. Appl. Genet., 2005, 111: 250-255. CrossRef
37. Mglinets A.V. Doklady Akademii nauk, 2008, 420: 556-558.
38. Saltoni S., Berville A. Characterization of the nuclear ribosomal DNA units and phylogeny Beta L. wild forms and cultivated beets. Theor. Appl. Genet., 1992, 83: 533-542. CrossRef
39. Jung C., Pillen K., Frese L., Fähr S., Melchinger A.E. Phylogenetic relationships between cultivated and wild species of the genus Beta revealed by DNA «fingerprinting». Theor. Appl. Genet., 1993, 86: 449-457. CrossRef
40. Kubis S., Heslop-Harrison J.S., Schmidt T. A family of differentially amplified repetitive DNA sequences in the genus Beta reveals genetic variation in Beta vulgaris subspecies and cultivars. J. Mol. Evol., 1997, 44: 310-320. CrossRef
41. Nishizawa S., Mikami T., Kubo T. Mitochondrial DNA phylogeny of cultivated and wild beets: relationships among cytoplasmic male-sterility-inducing and nonsterilizing cytoplasms. Genetics, 2007, 177: 1703-1712. CrossRef
42. Burenin V.I. Svekla — Beta L. (sistematika, genetika, iskhodnyi material i metody selektsii). Doktorskaya dissertatsiya [Beet — Beta L. (Systematics, Genetics, Source Material and Breeding Methods)]. Leningrad, 1983.