УДК 632.954:636.46:574.34:575:577.2

THE USE OF T-RFLP METHOD FOR STUDYING THE DYNAMICS OF SOIL MICROBIAL COMMUNITIES UNDER XENOBIOTICS TREATMENT

E.V. Pershina, E.E. Andronov, A.G. Pinaev, G.A. Akhtemova, V.A. Doumova, N.A. Provorov

The possibility of using T-RFLP (Terminal Restriction Fragment Length Polymorphism) and PCA (Principal Component Analysis) to quantify changes in soil microbial community composed of bacteria, fungi and archaea after acetone and 2,4-dichlorophenoxyacetic acid (2,4-D) treatment was investigated. The Shannon index calculation showed that diversity in fungi community increases after xenobiotics treatment, archaeal community has slightly decreasing indexes while indexes values for bacterial community remain constant. For the first time it was shown that PCA can be successfully used for investigation of the rate of succession for three components of the microbial community, maximum rates of succession speed were observed for fungi community.

Keywords: microbial community, T-RFLP, PCA, succession rate, xenobiotics.

ГНУ Всероссийский НИИсельскохозяйственной
микробиологии Россельхозакадемии,
196608 г. Санкт-Петербург—Пушкин, ш. Подбельского, 3,
e-mail: pershina.elizaveta@yandex.ru

Поступила в редакцию
16 февраля 2011 года

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