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Сельскохозяйственная биология, 2009, № 3, с. 114-117.

УДК 633.11:632.488

DIAGNOSTICS OF QUARANTINE PHYTOPATHOGENS BY THE PCR METHOD IN THE FLASH FORMAT

D.Yu. Ryazantsev¹, D.D. Abramov², S.K. Zavriev¹

On the basis of PCR in the FLASH format the authors developed the specific and sensitive diagnostic systems for a revealing of following quarantine phytopathogens: Ralstonia solanacearum, Clavibacter michiganensis subsp. Sepedonicus, Globodera pallida and G. rostochiensis, Plum Pox Potyvirus, Erwinia amylovora. The test-systems are suitable for routine diagnostics and were approved on wide range of isolates and race of phytopathogens.

Key words: quarantine plant pathogens, molecular diagnostics, fluorescent detection, PCR.

 

1Институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова РАН, 117997 г. Москва, ГСП-7, ул. Миклухо-Маклая, 16/10, e-mail: szavriev@ibch.ru; 2ЗАО «НПФ ДНК-Технология», 115446 г. Москва, Каширское ш., 23, корп. 5

Поступила в редакцию 7 апреля 2009 года

All-Russian Institute for Selection and Seed-Breeding of Vegetables All-Russian Selection and Technological Institute of Horticulture and Breeding Nursery Institute of Agricultural Biotechnology Joint Stock Company "GosNIISyntezBelok"