PLANT BIOLOGY
ANIMAL BIOLOGY
SUBSCRIPTION
E-SUBSCRIPTION
 
MAP
MAIN PAGE

 

 

 

 

Isakova E.P., Ovseichik E.A., Gessler N.N., Klein O.I., Rozumiy A.V., Deryabina Yu.I., Lukashenko V.S. The effect of phytase-containing feed supplements of fungal and bacterial origin on the biochemical blood features of Japanese quail (Coturnix japonica). Sel'skokhozyaistvennaya Biologiya [Agricultural Biology], 2025,Vol. 60, № 2, p. 345-360.
(how to cite this article)

doi: 10.15389/agrobiology.2025.2.345eng

UDC: 636.5.034:636.084:612.12

Acknowledgements:
The work was supported by the Russian Science Foundation grant No. 22-16-00093 dated 12.05.2022

 

THE EFFECT OF PHYTASE-CONTAINING FEED SUPPLEMENTS OF FUNGAL AND BACTERIAL ORIGIN ON THE BIOCHEMICAL BLOOD FEATURES OF JAPANESE QUAIL (Coturnix japonica)

E.P. Isakova1✉, E.A. Ovseichik2, N.N. Gessler1, O.I. Klein1,
A.V. Rozumiy1, Yu.I. Deryabina1, V.S. Lukashenko2

1Federal Research Center Fundamentals of Biotechnology RAS, 33/2, Leninskii prospect, Moscow, 119071 Russia, e-mail elen_iss@mail.ru (✉ corresponding author), gessler51@mail.ru, klein_olga@list.ru, rozumiy01@mail.ru, yul_der@mail.ru;
2Federal Scientific Center All-Russian Research and Technological Poultry Institute RAS, 10, ul. Ptitsegradskaya, Sergiev Posad, Moscow Province, 141311 Russia, e-mail ovseychik@vnitip.ru, lukashenko@vnitip.ru

ORCID:
Isakova E.P. orcid.org/0000-0003-3250-1814
Rozumiy A.V. orcid.org/0009-0009-2900-4834
Ovseichik E.A. orcid.org/0000-0002-2312-1388
Deryabina Yu.I. orcid.org/0000-0002-8850-4881
Gessler N.N. orcid.org/0000-0003-4121-4673
Lukashenko V.S. orcid.org/000-0002-0107-8235
Klein O.I. orcid.org/0000-0001-7427-0669

Final revision received June 10, 2024

Accepted August 12, 2024

Recently, the use of various phytase preparations in the poultry has drawn more attention. The modern market offers a wide range of phytase preparations, mainly phytases of microbial origin, namely class A (PhyA) and class C (PhyC). However, the enzymes upon passing along the gastrointestinal tract of the birds, significantly lose their activity due to acid denaturation and proteolysis. The technology of phytase encapsulation in yeast cells as microcontainers can reduce much enzyme losses, and increase its thermal stability. The extremophilic Yarrowia lipolytica yeast is a promising object for current biotechnology, in particular for the production of encapsulated phytase. The study first shows the evidence of high digestibility of phytate-containing products by Japanese Manchurian golden quails upon application of the encapsulated phytase of bacterial origin, compared to the control and commercial phytase of Ladozim proxy. The study was aimed at assaying the effect of feed supplements containing the phytases of fungal and bacterial origin on some biochemical features of Japanese quails. The experiment was performed in the Zagorskoe EPH Research Center, a branch of the VNITIP Scientific Research Center of the Russian Academy of Sciences (Sergiev Posad, Moscow Province) using quails (Coturnix japonica), the Manchurian golden breeds. In the diet of quails, there were used the cultivated pilot batches of a feed additive containing microencapsulated phytase of Obesumbacte-rium proteus and the commercial phytase of Ladozim proxy from Aspergillus ficuum (Biopreparat LLC, Russia) with an activity of 500 and 4,500 units of phytase/g, respectively. To obtain a feed additive of microencapsulated phytase from O. proteus, the modified method for obtaining the Yarrowia lipolytica Po1f pUV3-Or cells transformed with the phytase gene from O. proteus based on the pUVLT2 vector with a mitochondrial porin promoter VDAC (Voltage Dependent Anion Channel) was used. Six groups of one-day-old quails with 25 heads each were formed with different types of feed, namely group I (control+) got feed with a total phosphorus content of 0.45 % of available phosphorus without any additives; group II (control-) got the feed with a total phosphorus content of 0.35 % of available phosphorus without any additives; group III got the feed with the addition of O. proteus microencapsulated phytase at a dose of 500 units of phytase/kg of feed with a total phosphorus content of 0.35 % of available phosphorus; group IV got feed with the addition of O. proteus microencapsulated phytase at a dose of 500 units of phytase/kg with a total phosphorus content of 0.45 % of available phosphorus; Group V got the feed with an additive containing the commercial phytase Ladozim proxy from A. ficuum at a dose of 4,500 units of phytase/kg of feed with a total phosphorus content of 0.45 % of available phosphorus; group VI got the feed with an additive containing commercial phytase Ladozim proxy from A. ficuum at a dose of 4,500 units of phytase/kg of feed and a total phosphorus content of 0.35 % of available phosphorus. Upon removing quails from the experiment on the 42nd and 60th days, blood and tissue samples were taken from four heads of each group and some hematological parameters were assayed using an automatic URIT-3020 analyzer No. 3020E01663 (China). Tissue samples (pectoral and femoral muscles) were used to get histological preparations. Cryosections were analyzed using an Axio Imager D2 microscope at a 400½ magnification (Zeiss, Germany). At the first stage of life (42 days), the application of phytases in the diet decreased liver aminotransferase, alkaline phosphatase, and glucose levels, increased serum protein amount. When a commercial phytase was applied to the poultry diet with phosphorus deficiency at the age of 60 days, the level of aspartate aminotransferase, alkaline phosphatase, cholesterol, and triglycerides increased, while application of the encapsulated phytase from O. proteus raised the total protein amount and reduced the liver enzymes level. At the age of 60 days, under the conditions of complete total phosphorus, the feed additive increased the phosphorus level in the blood serum by 60 and 40% for the bacterial and fungal phytase groups, respectively. Microstructural assay of histological sections of the pectoral muscles showed that in the muscles of the quails getting phytase myofibrils had more arranged architecture with pronounced sarcomeres, stained myocyte nuclei, and transverse striation of fibers that indicated high quality and technological properties of meat. The obtained results indicated the beneficial effect of phytase preparations on the growth of the poultry and high digestibility by the quails. It allows us to conclude that application of the microencapsulated phytase of class D in the poultry feeding could be promising nd perspective, as it has the best kinetic characteristics compared to those in the phytases of classes PhyA and PhyC upon passing in the chymus of the poultry.

Keywords: quails, phytase, encapsulation, histological properties, biochemical parameters of blood.

 

REFERENCES

  1. Seal B.S., Lillehoj H.S., Donovan D.M., Gay C.G. Alternatives to antibiotics: a symposium on the challenges and solutions for animal production. Animal Health Research Reviews, 2013, 14(1): 78-87 CrossRef
  2. Ponte P.I.P., Prates J.A.M., Crespo J.P., Crespo D.G., Mourão J.L., Alves S.P., Bessa R.J.B., Chaveiro-Soares M.A., Ferreira L.M.A., Fontes C.M.G.A. Improving the lipid nutritive value of poultry meat through the incorporation of a dehydrated leguminous-based forage in the diet for broiler chicks. Poultry Science, 2008, 87(8): 1587-1594 CrossRef
  3. Givens I. Animal nutrition and lipids in animal products and their contribution to human intake and health. Nutrients, 2009, 1(1): 71-82 CrossRef
  4. Dong S., Li L., Fanyu Hao F., Fang Z., Zhong R., Wu J., Fang X. Improving quality of poultry and its meat products with probiotics, prebiotics, and phytoextracts. Poultry Science, 2024, 103(2): 103287 CrossRef
  5. Pu J., Hu J., Xiao J., Shugang Li S., Wang B., Wang J., Geng F. Integrated landscape of chicken egg chalaza proteomics. Poultry Science, 2024, 103(5): 103629 CrossRef
  6. Gagaev O.K., Bideev B.A., Demurova A.R. Materialy 7-y Mezhdunarodnoy nauchno-prakticheskoy konferentsii «Perspektivy razvitiya APK v sovremennykh usloviyakh» [Proc. 7 Int. Conf. «Prospects for the development of the agro-industrial complex in modern conditions»]. Vladikavkaz, 2017, vyp. 7: 66-69 (in Russ.).
  7. Osmanyan A.K., Slashcheva Yu.V., Komarchev A.S. Ptitsa i ptitseprodukty, 2022, 2: 28-32 CrossRef (in Russ.).
  8. Gržinić G., Piotrowicz-Cieślak A., Klimkowicz-Pawlas A., Górny R.L., Ławniczek-Wałczyk A., Piechowicz L., Olkowska E., Potrykus M., Tankiewicz M., Krupka M., Siebielec G., Wolska L. Intensive poultry farming: A review of the impact on the environment and human health. Science of The Total Environment, 2023, 858(part 3): 160014 CrossRef
  9. Maga J.A. Phytate: its chemistry, occurrence, food interactions, nutritional significance, and methods of analysis. Journal of Agricaltural Food Chemistry, 1982, 30(1): 1-9 CrossRef
  10. Raboy V. Myo-inositol-1,2,3,4,5,6-hexakisphosphate. Phytochemistry, 2003, 64(6): 1033-1043 CrossRef
  11. Azeke M.A., Egielewa S.J., Eigbogbo M.U., Ihimire I.G. Effect of germination on the phytase activity, phytate and total phosphorus contents of rice (Oryza sativa), maize (Zea mays), millet (Panicum miliaceum), sorghum (Sorghum bicolor) and wheat (Triticum aestivum). Journal of Food Science and Technology, 2011, 48: 724-729 CrossRef
  12. Lenkova T.N., Egorov I.A., Egorova T.A., Manukyan V.A., Vertiprakhov V.G., Grozina A.A. Ptitsa i ptitseprodukty, 2019, 1: 30-32 (in Russ.).
  13. Egorov I.A., Vertiprakhov V.G., Lenkova T.N. Manukyan V.A., Egorova T.A., Grozina A.A. The effects of diets supplemented with phytase on the productive performance, biochemical and morphological blood indices in broilers and layers of broiler preparental lines. Bioscience Research, 2020, 17(4): 2465-2471.
  14. Sysoeva I.G., Egorova T.A., Lenkova T.N. Ptitsevodstvo, 2020, 4: 35-39 CrossRef (in Russ.).
  15. Selle P.H., Ravindran V. Microbial phytase in poultry nutrition. Animal Feed Science and Technology, 2007, 135(1-2): 1-41 CrossRef
  16. Anchikov E.V. Phytase application in feeds for swine and poultry (review) Sel'skokhozyaistvennaya biologiya [Agricultural Biology],2008, 4: 3-14 (in Russ.).
  17. Lenkova T.N., Egorova T.A., Sysoeva I. G., Krivopishina L.V. Ptitsevodstvo, 2015, 10: 2-6 (in Russ.).
  18. Lenkova T.N., Egorov I.A., Egorova T.A., Manukyan V.A., Vertiprakhov V.G., Grozina A.A., Nikonov I.N., Philippova V.A., Yildirim E.A., Ilina L.A., Dubrovin A.V., Laptev G.Yu. Intestinal microbiota and broiler performance upon administration of phytase to increase phosphorus digestibility and nutrient utilization from feed.  Sel'skokhozyaistvennaya biologiya [Agricultural Biology], 2020, 55(2): 406-416 CrossRef
  19. Gessler N.N., Serdyuk E.G., Isakova E.P., Deryabina Y.I. Phytases and the prospects for their application (review). Applied Biochemistry and Microbiology, 2018, 54: 352-360 CrossRef
  20. Miller K.K., Alper H.S. Yarrowia lipolytica: more than an oleaginous workhorse. Applied Microbiology and Biochemistry,2019, 103: 9251-9262 CrossRef
  21. Serdyuk E.G., Isakova E.P., Gessler N.N., Trubnikova E.V., Antipov A.N., Deryabina Yu.I. Prikladnaya biokhimiya i mikrobiologiya, 2019, 55(5): 498-505 CrossRef (in Russ.).
  22. Isakova E.P., Gessler N.N., Deryabina Y.I. Comparative assay of phytase activity in Yarrowia lipolytica strains transformed with the neutrophilic phytase genome from Obesumbacterium proteus in batch cultivation. Applied Biochemistry and Microbiology, 2022, 58(1): 126-131 CrossRef
  23. Omotoso A.O., Reyer H., Oster M., Ponsuksili S., Wimmers K. Jejunal microbiota of broilers fed varying levels of mineral phosphorus. Poultry Science, 2023, 102(12): 103096 CrossRef
  24. Rezaeipour V., Barsalani A., Abdullahpour R. Effects of phytase supplementation on growth performance, jejunum morphology, liver health, and serum metabolites of Japanese quails fed sesame (Sesamum indicum) meal-based diets containing graded levels of protein. Tropical Animal Health and Production, 2016, 48: 1141-1146 CrossRef
  25. Vieira B.S., Caramori Junior J.G., Correa G.S.S., Colvara I.G., Brusamarelo E., Pereira T.V.S., Barbosa S.A.P.V., Oliveira C.F.S. Combination of phytase and citric acid, but not phytase alone, ensures regular rates of growth and bone mineralization in quails under severe phosphorus restriction. Journal of Animal Physiology and Animal Nutrition, 2019, 103(2): 555-563 CrossRef
  26. Alam S., Masood S., Zaneb H., Rabbani I., Khan R.U., Shah M., Ashraf S., Alhidary I.A. Effect of Bacillus cereus and phytase on the expression of musculoskeletal strength and gut health in Japanese quail (Coturnix japonica). The Journal of Poultry Science, 2020, 57(3): 200-204 CrossRef
  27. Danilova M.A., Epova E.Y., Trubnikova E.V., Badrutdinov N.V., Kokoreva A.S., Pusev M.S., Deryabina Y.I., Isakova E.P. Encapsulated phytase produced by recombinant Yarrowia lipolytica exhibits high efficiency on broiler chickens in low dosage. Applied Sciences, 2022, 12(23): 11999 CrossRef
  28. Hassan S., Habashy W., Ghoname M., Elnaggar A. Blood hematology and biochemical of four laying hen strains exposed to acute heat stress. International Journal of Biometeorol, 2023, 67: 675-686 CrossRef
  29. Ovseychik E.A., Klein O.I., Gessler N.N., Deryabina Y.I., Lukashenko V.S., Isakova E.P. The efficacy of encapsulated phytase based on recombinant Yarrowia lipolytica on quails’ zootechnic features and phosphorus assimilation. Veterinary Sciences, 2024, 11(2): 91 CrossRef
  30. AbuAli A.M., Mokhtar D.M., Ali R.A., Wassif E.T., Abdalla K.E.H. Morphological characteristics of the developing cecum of japanese quail (Coturnix coturnix japonica). Microscopy and Microanalysis, 2019, 25(4): 1017-1031 CrossRef
  31. Oster M., Reyer H., Trakooljul N., Weber F.M., Xi L., Muráni E., Ponsuksili S., Rodehutscord M., Bennewitz J., Wimmers K. Ileal transcriptome profiles of Japanese quail divergent in phosphorus utilization. International Journal of Molecular Sciences, 2020, 21(8): 2762 CrossRef
  32. Bhavsar K., Khire J.M. Current research and future perspectives of phytase bioprocessing. RSC Adv., 2014, 4: 26677-26691 CrossRef
  33. Mehraei Hamzekolaei M.H., Zamani Moghaddam A.K., Tohidifar S.S., Dehghani Samani A., Heydari A. The effects of transportation stress on Japanese quail (Coturnix Coturnix japonica) fed corn-based diet in comparison with wheat-based diet supplemented with xylanase and phytase. J. Anim. Physiol. Anim. Nutr., 2016, 100(4): 618-622 CrossRef
  34. Danilova M.A., Yu E., Trubnikova E.V., Shevelev A.B. A feed additive containing encapsulated 6‐phytase within recombinant Yarrowia lipolytica cells produced by cultivation on fat‐containing waste. Appl. Sci., 2022, 12(6): 3094 CrossRef
  35. Fan V.K., Vatnikov Yu.A., Lenchenko E.M. Agrarnaya nauka, 2021, 346(3): 21-25 CrossRef (in Russ.).
  36. Abd El-Hack M.E., Alagawany M., El-Sayed S.A.A., Fowler J. Influence of dietary inclusion of untreated or heat-treated Jatropha meal on productive and reproductive performances and biochemical blood parameters of laying Japanese quail. Poultry Science, 2017, 96(8): 2761-2767 CrossRef

 

back

 


CONTENTS

 

Full article PDF (Rus)