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doi: 10.15389/agrobiology.2019.2.347eng

UDC: 619:578:57.083.2:577.2

 

ONE-RUN REAL TIME PCR ASSAYS FOR THE DETECTION OF CAPRIPOXVIRUSES, FIELD ISOLATES AND VACCINE STRAINS OF LUMPY SKIN DISEASE VIRUS

A.V. Sprygin, Ya.E. Pestova, E.S. Kostrova, S.V. Kononova,
O.P. Byadovskaya, T.V. Zhbanova, A.V. Kononov

Federal Center for Animal Health Control, FGBU VNIIZZh, mkr. Yurievets, Vladimir, 600901 Russia, e-mail spriginav@mail.ru (✉ corresponding author), pestova@arriah.ru, kostrova@arriah.ru, kononova@arriah.ru, bjadovskaya@arriah.ru, zhbanova@arriah.ru, kononov@arriah.ru

ORCID:
Sprygin A.V. orcid.org/0000-0001-5982-3675
Byadovskaya O.P. orcid.org/0000-0002-8326-7151
Pestova Ya.E. orcid.org/0000-0002-7974-623X
Zhbanova T.V. orcid.org/0000-0002-9857-5915
Kostrova E.S. orcid.org/0000-0001-7150-7632
Kononov A.V. orcid.org/0000-0002-5523-3261
Kononova S.V. orcid.org/0000-0002-3932-2416

Received September 25, 2018

 

The cattle and sheep industry is economically important for sustainable growth. However, the increasing demand for livestock products drives animal population growth and risks for infection diseases. Lumpy skin disease (LSD) has recently expanded its historical range northward reaching countries that were never affected before. Prior to 2015 the territory of the Russian Federation was free of lumpy skin disease, whereas by 2017 Turkey, Serbia, Greece, Azerbaijan have reported incursions of this virus. Not only lumpy skin disease but also sheep pox has increased in incidence. Given this scenario, timely detection of these pathogens is key towards successful control and eradication. Moreover, diagnostic tools should detect both LSDV genome in the face of the use of live vaccine LSD virus strains and distinguish between the two. In this paper we report the development of a set of one-run real-time PCR assays to detect and differentiate between Capripoxvirus genome, field and vaccine LSD virus genomes. The assay for field LSD virus targets the 27 bp deletion in ORF126, the assay for vaccine LSD virus targets genetic signatures unique to Neethling vaccine strains, and the capripoxvirus assay targets the conserved P32 gene. The assays proved highly sensitive and specific. The set of PCRs was validated against a panel of 596 samples collected in the field, including whole blood, serum, skin lesions, nasal and ocular discharge, milk, lymph nodes, lungs, trachea, spleen and aborted calves. Using the assays reported here some samples obtained as part of national surveillance for LSD virus from animals exhibiting clinical signs consistent with LSDV turned out to be positive for vaccine LSD virus genome in 2017. This vaccine strain is highly likely to have derived from commercial live-attenuated vaccines against LSD virus. The way of introduction of a vaccine LSD virus strain into Russian cattle remains to be investigated.

Keywords: lumpy skin disease, vaccine, diagnostics, real-time PCR, genome, virus.

 

 

REFERENCES

  1. Coetzer J.A.W. Lumpy skin disease. In: Infectious diseases of livestock. J.A.W. Coetzer, R.C. Tustin (eds.). Oxford University Press, Cape Town, 2004.
  2. Hunter P., Wallace D. Lumpy skin disease in southern Africa: a review of the disease and aspects of control. Journal of the South African Veterinary Association, 2001, 72: 68-71 CrossRef
  3. Woods J.A. Lumpy skin disease: a review. Tropical Animal Health and Production, 1988, 20(1): 11-17.
  4. Sameea Yousefi P., Mardani K., Dalir-Naghadeh B., Jalilzadeh-Amin G. Epidemiological study of lumpy skin disease outbreaks in North-western Iran. Transboundary and Emerging Diseases, 2016, 64(6): 1782-1789 CrossRef
  5. Buller R.M., Arif B.M., Black D.N., Dumbell K.R., Esposito J.J., Lefkowitz E.J., McFad-den G., Moss B., Mercer A.A., Moyer R.W., Skinner M.A., Tripathy D.N. Family Poxviridae. In: Virus taxonomy: classification and nomenclature of viruses. Eighth Report of the International Committee on Taxonomy of Viruses. C.M. Fauquet, M.A. Mayo, J. Maniloff, U. Desselberger, L.A. Ball (eds.). Elsevier Academic Press, San Diego, 2005.
  6. Tulman E.R., Afonso C.L., Lu Z., Zsak L., Kutish G.F., Rock D.L. Genome of lumpy skin disease virus. Journal of Virology, 2001, 75(15): 7122-7130 CrossRef
  7. Elhaig M.M., Selim A., Mahmoud M. Lumpy skin disease in cattle: frequency of occurrence in a dairy farm and a preliminary assessment of its possible impact on Egyptian buffaloes. Onderstepoort Journal of Veterinary Research,2017, 84(1): e1-e6 CrossRef
  8. Gomo C., Kanonhuwa K., Godobo F., Tada O., Makuza S.M. Temporal and spatial distribution of lumpy skin disease (LSD) outbreaks in Mashonaland West Province of Zimbabwe from 2000 to 2013. Tropical Animal Health and Production, 2017, 49(3): 509-514 CrossRef
  9. Swiswa S., Masocha M., Pfukenyi D.M., Dhliwayo S., Chikerema S.M. Long-term changes in the spatial distribution of lumpy skin disease hotspots in Zimbabwe. Tropical Animal Health and Production, 2017, 49(1): 195-199 CrossRef
  10. Zeynalova S., Asadov K., Guliyev F., Vatani M., Aliyev V. Epizootology and molecular diagnosis of lumpy skin disease among livestock in Azerbaijan. Frontiers in Microbiology, 2016, 7: 1022 CrossRef
  11. Carn V.M., Kitching R.P. An investigation of possible routes of transmission of lumpy skin disease virus (Neethling). Epidemiology & Infection,1995, 114: 219-226 CrossRef
  12. Chihota C.M., Rennie L.F., Kitching R.P., Mellor P.S. Mechanical transmission of lumpy skin disease virus by Aedes aegypti (DipteraCulicidae). Epidemiology & Infection, 2001, 126: 317-321 CrossRef
  13. Lubinga J.C., Tuppurainen E.S., Coetzer J.A., Stoltsz W.H., Venter E.H. Transovarial passage and transmission of LSDV by Amblyomma hebraeum, Rhipicephalus appendiculatus and Rhipicephalus decoloratus ticks. Experimental and Applied Acarology, 2014, 62(1): 67-75 CrossRef
  14. Tuppurainen E.S., Venter E.H., Coetzer J.A., Bell-Sakyi L. Lumpy skin disease: attempted propagation in tick cell lines and presence of viral DNA in field ticks collected from naturally-infected cattle. Ticks and Tick-borne Diseases, 2015, 6(2): 134-140 CrossRef
  15. Tasioudi K.E., Antoniou S.E., Iliadou P., Sachpatzidis A., Plevraki E., Agianniotaki E.I., Fouki C., Mangana-Vougiouka O., Chondrokouki E., Dile C. Emergence of lumpy skin disease in Greece, 2015. Transboundary and Emerging Diseases, 2016, 63(3): 260-265 CrossRef
  16. Carn V.M., Kitching R.P. An investigation of possible routes of transmission of lumpy skin disease virus (Neethling). Epidemiology and Infection, 1995, 114(1): 219-226.
  17. Alkhamis M.A., Vander Waal K. Spatial and temporal epidemiology of lumpy skin disease in the Middle East, 2012-2015. Frontiers in Veterinary Science, 2016, 3: 19 CrossRef
  18. Mercier A., Arsevska E., Bournez L., Bronner A., Calavas D., Cauchard J., Falala S., Cau-four P., Tisseuil C., Lefrançois T., Lancelot R. Spread rate of lumpy skin disease in the Balkans, 2015-2016. Transboundary and Emerging Diseases, 2017, 65: 240-243 CrossRef
  19. Abutarbush S.M., Ababneh M.M., Al Zoubi I.G., Al Sheyab O.M., Al Zoubi M.G., Alekish M.O., Al Gharabat R.J. Lumpy skin disease in Jordan: disease emergence, clinical signs, complications and preliminary-associated economic losses. Transboundary and Emerging Diseases, 2015, 62(5): 549-554 CrossRef
  20. Biryuchenkova M.V., Timina A.M, Zinyakov N.G., Shcherbakov A.V.. Veterinariya segodnya, 2015, 4(15): 43-45 (in Russ.). 
  21. Sprygin A., Artyuchova E., Babin Y., Prutnikov P., Kostrova E., Byadovskaya O., Kononov A.  Epidemiological characterization of lumpy skin disease outbreaks in Russia in 2016. Transboundary and Emerging Diseases, 2018, 65(6): 1514-1521 CrossRef
  22. OIE. Follow-up report No. 20. Report Date: 28/12/2018. Available http://www.oie.int/wa-his_2/temp/reports/en_fup_0000029055_20181228_142744.pdf. No date.
  23. Bedeković T., Šimić I., Krešić N., Lojkić I. Detection of lumpy skin disease virus in skin lesions, blood, nasal swabs and milk following preventive vaccination. Transboundary and Emerging Diseases, 2018, 65(2): 491-496 CrossRef
  24. Kononov A., Prutnikov P., Shumilova I., Kononova S., Nesterov A., Byadovskaya O., Pestova Y., Diev V., Sprygin A1. Determination of lumpy skin disease virus in bovine meat and offal products following experimental infection. Transboundary and Emerging Diseases, 2019, 00: 1-9 CrossRef
  25. Tuppurainen E.S., Venter E.H., Shisler J.L., Gari G., Mekonnen G.A., Juleff N., Lyons N.A., De Clercq K., Upton C., Bowden T.R., Babiuk S., Babiuk L.A. Capripoxvirus diseases: current status and opportunities for control. Transboundary and Emerging Diseases, 2017, 64(3): 729-745 CrossRef
  26. Bedeković T., Šimić I., Krešić N., Lojkić I. Detection of lumpy skin disease virus in skin lesions, blood, nasal swabs and milk following preventive vaccination. Transboundary and Emerging Diseases, 2018, 65(2): 491-496 CrossRef
  27. Pestova Ya.E, Artyukhova E.E., Kostrova E.E, Shumilova I.N., Kononov A.V., Sprygin A.V. Real time PCR for the detection of field isolates of lumpy skin disease virus in clinical samples from cattle. Agricultural Biology [Sel’skokhozyaistvennaya Biologiya], 2018, 53(2): 422-429 CrossRef
  28. Lin L.I. A concordance correlation coefficient to evaluate reproducibility. Biometrics, 1989, 45(1): 255-268 CrossRef
  29. Ireland D.C., Binepal Y.S. Improved detection of capripoxvirus in biopsy samples by PCR. Journal of Virological Methods, 1998, 74(1): 1-7 CrossRef
  30. Stubbs S., Oura C.A., Henstock M., Bowden T.R., King D.P., Tuppurainen E.S. Validation of a high-throughput real-time polymerase chain reaction assay for the detection of capripoxviral DNA. Journal of Virological Methods,2012, 179(2): 419-422 CrossRef
  31. Vidanovic D., Sekler M., Petrovic T., Debekjak Z., Vaskovic N., Matovic K., Hoffmann B. Real-time PCR assays for the species detection of field Balkan strains of lumpy skin disease virus. Acta Veterinaria Belgrade,2016, 66(4): 444-454 CrossRef
  32. Agianniotaki E.I., Chaintoutis S.C., Haegeman A., Tasioudi K.E., De Leeuw I., Katsoulos P.D., Sachpatzidis A., De Clercq K., Alexandropoulos T., Polizopoulou Z.S., Chondrokouki E.D., Dovas C.I. Development and validation of a TaqMan probe-based real-time PCR method for the differentiation of wild type lumpy skin disease virus from vaccine virus strains. Journal of Virological Methods, 2017, 249: 48-57 CrossRef
  33. Babiuk S., Bowden T.R., Parkyn G., Dalman B., Manning L., Neufeld J., Embury-Hyatt C., Copps J., Boyle D.B. Quantification of lumpy skin disease virus following experimental infection in cattle. Transboundary and Emerging Diseases,2008, 55(7): 299-307 CrossRef
  34. Sprygin A., Pestova Y., Prutnikov P., Kononov A. Detection of vaccine-like lumpy skin disease virus in cattle and Musca domestica L. flies in an outbreak of lumpy skin disease in Russia in 2017. Transboundary and Emerging Diseases, 2018, 65(5): 1137-1144 CrossRef
  35. Sprygin A.V, Kononov A.V., B'yadovskaya O.P. et al. Oligonukleotidnye praimery i fluorestsentno-mechenyi zond, sposob i test-sistema PTSR v rezhime real'nogo vremeni dlya vyyavleniya genoma kapripoksvirusov.  Pat. 2658493, FGBU «Federal'nyi tsentr okhrany zdorov'ya zhivotnykh» (FGBU «VNIIZZH»). № 2017130118. Zayavl. 24.08.2017. Opubl. 21.06.2018. Byul. № 18 [Oligonucleotide primers and labeled fluorescent probe, method and real-time PCR test system for detecting the genome of capripoxviruses. No. 2017130118. Appl. 24.08.2017. Publ. 21.06.2018. Bul. No. 18] (in Russ.).
  36. Sprygin A.V, Kononov A.V., B'yadovskaya O.P. et al. Oligonukleotidnye praimery i fluorestsentno-mechenyi zond, sposob i test-sistema dlya vyyavleniya genoma polevykh izolyatov virusa zaraznogo uzelkovogo dermatita (nodulyarnogo dermatita) KRS v reaktsii polimeraznoi tsepnoi reaktsii v rezhime real'nogo vremeni.  Pat. 2668398, FGBU «Federal'nyi tsentr okhrany zdorov'ya zhivotnykh» (FGBU «VNIIZZH»). № 2017124318. Zayavl. 07.07.2017. Opubl. 28.09.2018. Byul. № 28 [Oligonucleotide primers and labeled fluorescent probe, method and test system for detecting the genome of field isolates of infectious nodular dermatitis virus (nodular dermatitis) of cattle in real-time polymerase chain reaction. No. 2017124318. Appl. 07.07.2017. Publ. 28.09.2018. Bul. No. 6](in Russ.).

 

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