PLANT BIOLOGY
ANIMAL BIOLOGY
SUBSCRIPTION
E-SUBSCRIPTION
 
MAP
MAIN PAGE

 

 

 

 

Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2019, Vol. 54, № 2, p. 326-336.
(how to cite this article)

doi: 10.15389/agrobiology.2019.2.326eng

UDC: 636.5:636.085.8

 

LUPINE IS APPLICABLE IN DIETS FOR LAYER CHICKEN OF PARENTAL FLOCK

E.N. Andrianova1, I.A. Egorov1, E.N. Grigoryeva1, A.N. Shevyakov1,
V.V. Pronin2

1Federal Scientific Center All-Russian Research and Technological Poultry Institute RAS, 10, ul. Ptitsegradskaya, Sergiev Posad, Moscow Province, 141311 Russia, e-mail andrianova@vnitip.ru (corresponding author ✉), olga@vnitip.ru, vnitip@vnitip.ru, alex.shevy@mail.ru;
2Federal Center for Animal Health Control, FGBU VNIIZZh, mkr. Yurievets, Vladimir, 600901 Russia, e-mail proninvv63@mail.ru

ORCID:
Andrianova E.N. orcid.org/0000-0002-6769-6351
Shevyakov A.N. orcid.org/0000-0001-7117-1067
Egorov I.A. orcid.org/0000-0001-9122-9553
Pronin V.V. orcid.org/0000-0002-6240-3062

Received September 21, 2018

 

Climatic conditions in most Russian regions are unfavorable for the cultivation of soy beans which are considered the best protein source in diets for all types of poultry. Soya yield in Russian soya-producing regions (Far East, Krasnodar Krai, and some other southern territories) cannot provide the growing poultry production with this important protein source. The alternatives for soybean meal and other soya products are therefore in need; domestically selected low-alkaloid cultivars of white and narrow-leaf lupine are increasingly gaining importance as vegetable protein sources. Feed-grade lupine usually contains up to 42 % of crude protein. The disadvantages of lupine are high contents of fiber (12.5-16.0 %) and lignin (0.9 %), and the presence of alkaloids. Alkaloid content in sweet lupine cultivars is 0.008-0.12 %, in bitter cultivars 1-3 %. Chemical and amino acid composition of white lupine modern cultivars was earlier determined, and their efficiency in poultry diets was studied in vivo. It was found that the grain of low-alkaloid cultivars Gamma, Dega, Dikaf 14 can be included into the diets for poultry at 15-20 % dosage. Supplementation of lupine-containing diets with proper enzyme preparations can improve the digestibility of dietary nutrients and poultry performance. Dehulling of lupine grain decreases fiber content and increases protein content in concentrated lupine-based protein feeds; the latter in dehulled lupine is close to that in soybean products. This original research for the first time proves the possibility of soybean and sunflower meals substitution for white low-alkaloid lupine cultivar Dega the in diets for parental flock of laying hens. The trial was performed on 5 groups of parental White Leghorn layers (cross SP 789) from 184 to 365 days of age fed balanced diets containing 0; 5; 7; 10; and 15 % of dehulled Dega lupine grain (39.61 % crude protein, 5.60 % crude fiber). It was found that 5-10 % lupine does not impair livability and productivity parameters in layers. Lupine was found to influence the intensity of lay, egg fertility, hatch of chicks, micromorphology of liver in hens. The substitution of soy for lupine (7, 10, and 15 %) improved egg production and egg weight output per hen by 1.51 and 7.10 %; 3.31 and 1.64 %; 6.56 and 3.64 %, respectively, in compare to control; feed expenses per 1 kg egg weight in these groups was 0.9; 4.07; and 1.81 % lower. The percentages of infertile eggs and early embryonic deaths in using 10 and 15 % lupine were lower compared to control, evidencing the absence of negative impact of lupine Dega on early embryonic development. The highest doses of lupine did not increase the incidence of late embryonic deaths in eggs from layers aged 47 weeks: the percentages of late embryonic deaths and weak chicks from layers fed 15 % lupine were 4 and 5 % vs. 9 and 8 % in control. The histological investigation of liver revealed no significant differences between lupine-fed and control layers; nucleoplasmic ratio in the hepatocytes was similar in all treatments. In all treatments liver had no abnormalities, the connective tissues were poorly developed and located in the peripheral segments of the liver (where it forms a capsule) and near the portal triad. The hepatic plate structure is well developed, and the tortuous plates are radially oriented. The clusters of blood cells are seen in the lumen of the central veins and sinuous capillaries. Hepatocytes are not clearly bordered, polygonal in shape; nuclei centered or sometimes slightly peripherally shifted, round or oviform, has 1-4 nucleoli. Cytoplasm is unevenly stained, granular; lymphoid cells are found in the stroma and parenchyma. The results of histological investigation of liver, productivity parameters in layers, and the efficiency of incubation of eggs obtained evidence that 5-15 % of Dega lupine dehulled grain as a protein source in diets of laying hens from parental flock does not impair productivity and rendered no cytotoxic effects on the liver of hens. This cultivar of white lupine can be recommended as a dietary protein source both for commercial and parental layer flocks.

Keywords: white lupine, alkaloids, laying hens, productivity, egg fertility, hatchability, liver histomorphology.

 

 

REFERENCES

  1. Egorov I., Andrianova E., Prisyazhnaya L., Shtele A. Ptitsevodstvo, 2009, 9: 25-27 (in Russ.).
  2. Egorov I.A., Andrianova E.N., Tsygutkin A.S., Shtele A.L. Dostizheniya nauki i tekhniki APK, 2010, 9: 36-38 (in Russ.).
  3. Yagovenko T., Afonina E. Kombikorma, 2018, 3: 66-68 (in Russ.).
  4. Egorov I.A., Lenkova T.N., Manukyan V.A. et al. Nastavleniya po ispol'zovaniyu netraditsion-nykh kormov v ratsionakh ptitsy /Pod red. V.I. Fisinina [Instructions on the use of unconventional feed in poultry diets. V.I. Fisinin (ed.)]. Sergiev Posad, 2016 (in Russ.).
  5. Vashchekin E.P., D'yachenko A.P. Metabolism and semen production of sires using in the ration the ground bean of blue lupine. Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2008, 4: 58-63 (in Russ.).
  6. Shtele A.L. Belyi lyupin, 2015, 1: 15-20 (in Russ.).
  7. Kubiś M., Kaczmarek S.A., Nowaczewski S., Adamski M., Hejdysz M., Rutkowski A. Influence of graded inclusion of white lupin (Lupinus albus) meal on performance, nutrient digestibility and ileal viscosity of laying hens. British Poultry Science, 2018, 59(4): 477-484 CrossRef
  8. Beyene G., Ameha N., Urge M., Estifanos A. Replacing soybean meal with processed lupin (Lupinus albus) meal as poultry layers feed.Livestock Research for Rural Development,2014, 26(11): 204.
  9. Laudadio V., Tufarelli V. Influence of substituting dietary soybean meal for dehulled-micronized lupin (Lupinus albus cv. Multitalia) on early phase laying hens production and egg quality. Livestock Science, 2011, 140(1-3): 184-188 CrossRef
  10. Krawczyk M., Przywitowski M., Mikulski D. Effect of yellow lupine (L. luteus) on the egg yolk fatty acid profile, the physicochemical and sensory properties of eggs, and laying hen performance. Poultry Science, 2015, 94(6): 1360-1367 CrossRef
  11. Park J.H., Lee S.I., Kim I.H. Effects of lupin seed supplementation on egg production performance, and qualitative egg traits in laying hens. Veterinarni Medicina, 2016, 61(12): 701-709 CrossRef 
  12. Rutkowski A., Hejdysz M., Kaczmarek S., Adamski M., Nowaczewski S., Jamroz D. The effect of addition of yellow lupin seeds (Lupinus luteus L.) to laying hen diets on performance and egg quality parameters. Journal of Animal and Feed Sciences, 2017, 26(3): 247-256 CrossRef 
  13. Jeroch H., Kozlowski K., Mikulski D., Jamroz D., Schoene F., Zdunczyk Z. Lupines (Lupinus spp.) as a protein feedstuff for poultry. 2. Results of poultry feeding trials and recommendations on diet formulation. European Poultry Science, 2016, 80: 166 CrossRef
  14. Jamroz D., Kubizna J. Harmful substances in legume seeds — their negative and beneficial properties. Polish Journal of Veterinary Sciences, 2008, 11: 389-404.
  15. Mierlita D., Simeanu D., Pop I.M., Criste F., Pop C., Simeanu C., Lup F. Chemical composition and nutritional evaluation of the lupine seeds (Lupinus albus L.) from low-alkaloid varieties. Revista de Chemie, 2018, 69(2): 453-458.
  16. Hejdysz M., Kaczmarek S.A., Kubis M., Jamroz D., Kasprowicz-Potocka M., Zaworska A., Rutkowski A. Effect of increasing levels of raw and extruded narrow-leafed lupin seeds in broiler diet on performance parameters, nutrient digestibility and AME(N) value of diet. Journal of Animal and Feed Sciences, 2018, 27(1): 55-64 CrossRef
  17. Lee M.R.F., Parkinson S., Fleming H.R., Theobald V.J., Leemans D.K., Burgess T. The potential of blue lupins as a protein source in the diets of laying hens. Veterinary and Animal Science, 2016, 1-2: 29-35 CrossRef
  18. Hammershøj M., Steenfeldt S. Effects of blue lupin (Lupinus angustifolius) in organic layer diets and supplementation with foraging material on egg production and some egg quality parameters. Poultry Science, 2005, 84(5): 723-733 CrossRef 
  19. Andrianova E.N. Nauchnoe obosnovanie povysheniya effektivnosti ispol'zovaniya kormov pri proizvodstve yaits i myasa ptitsy. Dokt. dis. [Scientific rationale for improving the efficiency of feed use in production of poultry eggs and meat. DSci Thesis]. Sergiev Posad, 2013 (in Russ.).
  20. Tsygutkin A.S., Shtele A.L., Andrianova E.N., Medvedeva N.V. Dostizheniya nauki i tekhniki APK, 2011, 9: 41-43 (in Russ.).
  21. Artyukhov A.I., Yagovenko T.V., Afonina E.V., Troshina L.V. Kolichestvennoe opredelenie alkaloidov v lyupine: metodicheskie rekomendatsii [Quantitative determination of alkaloids in lupine: methodical recommendations]. Bryansk, 2012 (in Russ.).
  22. Mera-Zúñiga F., Pro-Martínez A., Zamora-Natera J.F., Sosa-Montes E., Guerrero-Rodrí-guez J.D., Mendoza-Pedroza S.I., Cuca-García J.M., López-Romero R.M., Chan-Díaz D., Becerril-Pérez C.M., Vargas-Galicia A.J., Bautista-Ortega J. Soybean meal substitution by dehulled lupine (Lupinus angustifolius) with enzymes in broiler diets. Asian-Australasian Journal of Animal Science, 2018, 32(4): 564-573 CrossRef
  23. Egorov I.A., Manukyan V.A., Okolelova T.M. et al. Rukovodstvo po kormleniyu sel'skokhozyaistvennoi ptitsy /Pod red. V.I. Fisinina, I.A. Egorova [Poultry feeding — a guideline. V.I. Fisinin, I.A. Egorov (eds.)]. Sergiev Posad, 2018 (in Russ.).
  24. Tashke K. Vvedenie v kolichestvennuyu tsito-gistologicheskuyu morfologiyu [Introduction to quantitative cyto-histological morphology]. Rumyniya, 1980 (in Russ.). 
  25. Lakin G.F. Biometriya [Biometrics]. Moscow, 1990 (in Russ.).   
  26. Plokhinskii N.A. Rukovodstvo po biometrii dlya zootekhnikov [Guide to biometrics for livestock specialists]. Moscow, 1969 (in Russ.).     
  27. Andrianova E.N., Krivopishina L.V., Chvanova O.A., Tsygutkin A.S. Ptitsa i ptitseprodukty, 2015, 5: 47-49 (in Russ.).   
  28. Konopleva A.P., Andreeva A.A., Trokholis T.N. Sbornik nauchnykh trudov VNITIP, 2012, 86: 24-35 (in Russ.).   
  29. Avtandilov G.G. Yabluchanskii N.I., Gubenko V.G. Sistemnaya stereometriya v izuchenii patologicheskogo protsessa [System stereometry in the study of the pathological process]. Moscow, 1981 (in Russ.).    
  30. Kolda J., Komarek V. Anatomia domacich ptaku. Praha, 1958: 146-154.
  31. Hamodi H.M., Abed A.A., Taha A.M. Comparative anatomical, histological and histochemical study of the liver in three species of birds. Raf. J. Sci., 2013, 24(5): 12-23.
  32. Hochleithner M., Hochleithner C., HarrisonL.D. Evaluating and treating the liver. Ch. 15. In: Clinical Avian Medicine, Vol. 1. Spix Publishing Inc., Palm Beach, Florida, 2006: 441-449.
  33. Hoffman C.E., Kraupe P., Weiss L., Wittman J. Avian ATP citrate (pro-3s)-lyase. Hoppe-Seylers Z. Physiol. Chem., 1980, 361(7): 1117-1119.
  34. Noyan A., Lossow W.J., Brot N., Chaikoff I.L. Pathway and form of absorption of palmitic acid in the chicken. J. Lipid Res., 1964, 5(4): 538-541.

 

back

 


CONTENTS

 

 

Full article PDF (Rus)

Full article PDF (Eng)