doi: 10.15389/agrobiology.2018.2.318eng

UDC 636.32/.38:636.018:591.463.1

The authors would like to thank the Shuli research station, Chahamahal Bakhtiari Province, for assisting in sample collection.


AND Lori Bakhtiari RAMS

S.A. Hoseini1, S. Mohammadzadeh1, A. Kadivar2

1Department of animal science, Lorestan University,Lorestan Province, Khorramabad, A81, Iran, e-mail (✉ corresponding author),;
2Shahrekord University, Veterinary school, Animal physiology Department,Chaharmahal and Bakhtiari Province, Shahrekord, University, Rahbar Blvd, Iran, e-mail (✉ corresponding author)
The authors declare no conflict of interests

Received August 7, 2017


One of the important aims in animal breeding is using the advantages of bio diversity. Romanov sheep is Russian native breed and has prominent features, such as high percent of twinning. Lori-Bakhtiari breed is dual-purpose (meat and wool) in Iran. Regarding the governmental policies, confluence between these two breeds is on the agenda. The aim of this research is to study comparatively some of the sperm features of rams in both breeds in terms of concentration, motility, morphometry. Semen samples of Lori and Romanov rams were collected in husbandry research station known as Shuli in Chaharmahal Bakhtiari Province in Iran. In this experiment, sperm parameters in Romanov ram including large diameter, small diameter, perimeter and area of head, tail length, mid piece and end piece length were estimated as 9.16±0.37 μ, 4.77±0.41 μ, 24.47±0.97 μ, 24.89±1.94 μ2, 57.59±1.50 μ, 15.40±1.20 μ and 42.19±2.10 μ, respectively. A significant difference was observed between the large diameter and head area of sperm, while the small diameter and perimeter of sperm were similar. Motility of type A in Romanov rams was significantly higher. The tail and mid piece of Romanov sperms was significantly taller than these parts in Lori’s rams. It seems that taller length of the mid piece and more mitochondria concentration are the causes of higher motility in Romanov spermatozoa.

Keywords: ram, Romanov breed, Lori Bakhtiari breed, sperm, motility, morphology.


Full article (Rus)

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  1. Salimi R., Asadi H. Effective parameters on reproduction. Danesh Parvar, 2014.
  2. Kamkar K. Sheep husbandry. Farhang Jame Pub., 2008.
  3. Soori M., Nurian Sarvar E. Sheep husbandary. Iran Razi University, Kermanshah, 2007.
  4. Louda F., Doney J.M., Štolc L., Krízek J., Šmerha J. The development of sexual activity and semen production in ram lambs of two prolific breeds: Romanov and Finnish Landrace. Anim. Prod., 1981, 33(2): 143-148 CrossRef
  5. Büyükleblebici S., Tuncer P.B., Bucak M.N., Tasdemir U., Eken A., Büyükleblebici O., Durmaz E., Sariözkan S., Endirlik B.Ü. Comparing ethylene glycol with glycerol and with or without dithiothreitol and sucrose for cryopreservation of bull semen in egg-yolk containing extenders. Cryobiology, 2014, 69: 74-78 CrossRef
  6. Mohammadi G., Mahdiun H., Gurani Nezhad S. Improvement of semen evaluation techniques. Veterinary Journal and Laboratory, 2011, 3: 135-145.
  7. Tajik P. Tajik P., Ghasemzadeh-Nava H., Lotfollahzadeh S., Shirzad M.R. Assessment of live/dead and protoplasmic droplets in epididymal sperm cells in Iranian Zell rams. J. Fac. of Vet. Med., University of Tehran, 2003, 58(1): 25-28 (abs. in English).
  8. Wainer R., Albert M., Dorion A., Bailly M., Bergère M., Lombroso R., Gombault M., Selva J. Influence of the number of motile spermatozoa inseminated and of their morphology on the success of intrauterine insemination. Hum. Reprod., 2004, 19(9): 2060-2065 CrossRef
  9. Lindheim S.R., Barad D.H., Zinger M., Witt B., Amin H., Cohen B., Fisch H., Barg P. Abnormal sperm morphology is highly predictive of pregnancy outcome during controlled ovarian hyperstimulation and intrauterine insemination. J. Assist. Reprod. Gen., 1996, 13(7): 569-572 CrossRef
  10. Fazli Nezhd J., Mamoeii M., Kheradmand A., Sukhteh Zari A. The effect of melatonin on testicular circumference and semen characteristics in non-breeding season in Lori-Bakhtiari ram. Journal of Veterinary Research, 2016, (71)1: 27-32.
  11. Lafortune E., Blanc M.R., Orgeur P., Pelletier J., Perreau C., Terqui M., Hochereau-de Reviers M.T. A comparison of the changes in LH, FSH and testosterone in spring-born ram lambs of two different breeds. Reprod. Nutr. Dev., 1984, 24: 947-952.
  12. Druart X., Cognié J., Baril G., Clément F., Dacheux J.-L., Gatti J.-L. In vivo imaging of in situ motility of fresh and liquid stored ram spermatozoa in the ewe genital tract. Reproduction, 2009, 138(1): 45-53 CrossRef
  13. Noakes D., Parkinson T.J., England G.C., Arthur G.H. Arthur's veterinary reproduction and obstetrics. Saunders Ltd., 2001.
  14. Mohammadi G., Barati F. Artificial insemination in farm animals. Shahid Chamran University, Ahvaz, 2009.
  15. Yániz J., Capistrós S., Vicente-Fiel S., Soler C., De Murga J.N., Santolaria P. Study of nuclear and acrosomal sperm morphometry in ram using a computer-assisted sperm morphometry analysis fluorescence (CASMA-F) method. Theriogenology, 2014, 82(6): 921-924 CrossRef
  16. Gravance C.G., Champion Z.J., Casey P.J. Computer-assisted sperm head morphometry analysis (ASMA) of cryopreserved ram spermatozoa. Theriogenology, 1998, 49(6): 1219-1230 CrossRef
  17. Yániz J.L., Capistrós, S., Vicente-Fiel S., Hidalgo C.O., Santolaria P. A comparative study of the morphometry of sperm head components in cattle, sheep, and pigs with a computer-assisted fluorescence method. Asian J. Androl., 2016, 18(6): 840-843 CrossRef