Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2017, V. 52, № 2, pp. 223-231
(how to cite this article)

doi: 10.15389/agrobiology.2017.2.223eng

UDC 636.2:576.08:577.29

 

OMICS-TECHNOLOGIES TO ANALYZE INDIVIDUAL BOVINE OOCYTES
FOR IDENTIFICATION OF DEVELOPMENTAL OOCYTE COMPETENCE BIOMARKERS

S. Uzbekova

Laboratory «Integrative Ovarian Biology», Research Unit «Physiology of Reproduction and Behavior», UMR INRA 85-CNRS 7247-Université de Tours, Department of Animal Physiology and Livestock Systems, French National Institute for Agricultural Research (INRA), Centre Val de Loire 37380 Nouzilly France, e-mail svetlana.uzbekova@inra.fr

The authors declare no conflict of interests

ORCID:
Uzbekova S. orcid.org/0000-0002-6989-6904

Received December 30, 2016

 

Oocyte quality is a capacity to be fertilized and to develop into viable embryo and this is crucial for reproductive biotechnologies in farm animals.  Technical progress and possible miniaturization of «omics» technologies made possible application of transcriptomic, proteomic and lipidomic methodologies to single oocyte, and thus to search molecular factors representing possible markers of oocyte quality. Oocyte quality is determined by its follicular environment and affects transcript, protein and lipid composition of an oocyte, which has to progress through maturation — a final step before fertilization, crucial for the acquisition of oocyte developmental competence. Here we describe the examples of «omics» analysis performed on single bovine oocytes and their neighboring cumulus cells through the comparison of the oocytes with different competence to maturate and to develop blastocyst in vitro. In particular, we focus to original technologies of proteomics and lipidomics based on mass spectrometry phenotyping of intact cells and identification of molecular biomarkers.

Keywords: «omics» technologies, single oocyte, bovine.

 

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