doi: 10.15389/agrobiology.2017.2.242eng

UDC 636.3.033:591.463.1:57.086

 

ADDITIVES USED IN EXTENDERS TO IMPROVE THE FREEZABILITY OF RAM SEMEN IN RECENT YEARS: a mini review

Y. Öztürkler, U.Ç. Ari

Department of Reproduction & Artificial Insemination, Clinical Sciences Section, Veterinary Faculty,KafkasUniversity, 36000, Kars, Turkey,
e-mail yavuzozturkler@hotmail.com (corresponding author)

The authors declare no conflict of interests

Received December 30, 2016

 

One of the most important reasons why artificial insemination does not spread as much in cattle as sheep is that ram sperm is highly fragile against cryodamage and consequently the optimum fertility results can not be obtained from cervical inseminations compared to laparoscopic insemination. To establish an ideal ram sperm freezing model, many studies have been carried out for more than 60 years and various methods have been tested by making great efforts. One of the topics where these tests have been done intensively in recent years are additives used in freezing extenders in order to increase the tolerance of sperm against oxidative stress in frozen-thawed ram semen. In this review, covering the studies carried out between the years 2000-2016, we have mostly compiled the additives used in ram semen freezing media which have given better results compared to others supplements. It is understood that, usually, seminal plasma and proteins, thiol compounds, enzymatic antioxidants, sugars, fatty acids and vitamins have been studied as additive agents in recent years. As a result, this review suggests us that further studies are needed to explore the novel techniques and new additives, and their combinations should be tested in different doses in order to install an ideal cryopreservation template for ram semen. Furthermore, even though addition of various compounds to freezing media have improved freezability of ram semen in experimental conditions, anyway these results must be confirmed in field studies. Therefore, the value of sperm which potential fertility is predicted from laboratory survey must be compared with conception or lambing rates.

Keywords: ram semen, additives, freezability, extender.

 

Full article (Rus)

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REFERENCES

  1. Quinn P.J., White I.G., Cleland K.W. Chemical and ultrastructural changes in ram spermatozoa after washing, cold shock and freezing. J. Reprod. Fertil., 1969, 18: 209-220.
  2. King M.E., McKelvey W.A.C., Dingwall W.S., Matthews K.P., Gebbie F.E., Mylne M.J.A., StewartRobinson J.J. Lambing rates and litter sizes following intrauterine or cervical insemination of frozen-thawed semen with or without oxytocin administration. Theriogenology, 2004, 62: 1236-1244 CrossRef
  3. Woelders H., Hiemstra S.J. The potential of cryopreservation and reproductive technologies for animal genetic resources conservation strategies. Cryobiology, 2011, 63(3): 316-317 CrossRef
  4. Rodríguez-Martínez H. Sperm biotechnologies in domestic species: state of the art. Anim. Reprod., 2013, 10(3): 268-276.
  5. Baran A., Ak K., Ileri I.K., Soylu M.K. Effects of adding bull seminal plasma to ram semen extenders on post-thaw spermatozoa motility and morphology. Indian Vet. J., 2004, 81(7): 780-783.
  6. Marti E., Marti J.I., Muino-Blanco T., Cebrian-Perez J.A. Effect of the cryopreservation process on the activity and immunolocalization of antioxidant enzymes in ram spermatozoa. J. Androl., 2008, 29: 4 CrossRef
  7. Leahy T., de Graaf S.P. Seminal plasma and its effect on ruminant spermatozoa during processing. Reprod. Domest. Anim., 2012, 47(4): 207-213 CrossRef
  8. de Graaf S.P., Leahy T., Marti J., Evans G., Maxwell W.M.C. Application of seminal plasma in sex-sorting and sperm cryopreservation. Theriogenology, 2008, 70(8): 1360-1363 CrossRef
  9. Alvarez J.G., Storey B.T. Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol. Reprod. Dev., 1995, 42(3): 334-346 CrossRef
  10. Ari U.Ç., Öztürkler Y. Oxidative stress during long and short term storage of sperm and usage of antioxidant. Turkiye Klinikleri J. Reprod. Artif. Insemin.-Special Topics, 2015, 1(3): 16-21.
  11. Öztürkler Y., Yildiz S., Güngör Ö., Pancarci SM., Kaçar C., Ari U.Ç. The effects of L-ergothioneine and L-ascorbic acid on in vitro maturation and embryonic development in sheep oocytes. Kafkas Univ. Vet. Fak. Derg., 2010, 16: 757-763.
  12. Bansal A.K., Bilaspuri G.S. Impacts of oxidative stress and antioxidants on semen functions. Veterinary Medicine International, 2011, 2011: Article ID 686137 CrossRef
  13. Uysal O., Bucak M.N. Effects of oxidized glutathione, bovine serum albumin, cysteine and lycopene on the quality of frozen-thawed ram semen. Acta Vet. Brno, 2007, 76(3): 383-390 CrossRef
  14. Bucak M.N., Atessahin A., Yüce A. Effect of anti-oxidants and oxidative stress parameters on ram semen after the freeze-thawing process. Small Ruminant Res., 2008, 75: 128-134 CrossRef
  15. Anghel A., Zamfirescu S., Coprean D., Sogorescu E. The effects of cysteine, bovine serum albumin and vitamin E on the qualitative parameters of frozen-thawed ram semen. Annals of the Romanian Society for Cell Biology, 2009, 14(2): 97-103.
  16. Ari U.Ç., Kulaksiz R., Öztürkler Y. Freezability of Tushin ram semen extended with goat or cow milk based extenders. Reprod. Domest. Anim., 2011, 46(6): 975-979 CrossRef
  17. Ari U.Ç., Kulaksiz R., Öztürkler Y., Lehimcioglu N.C., Yildiz S. Effect of N-acetylcysteine (NAC) on post-thaw semen quality of Tushin rams. Kafkas Univ. Vet. Fak. Derg., 2016., 22(6): 883-887.
  18. Yildiz S., Öztürkler Y., Ari U.Ç., Lehimcioglu N.C., Atakisi E., Kulaksiz R. The effects of L-ergothioneine, N-acetylcystein and cystein on freezing of ram semen. Kafkas Univ. Vet. Fak. Derg., 2015, 21(1): 81-86.
  19. Agarwal A., Gupta S., Sharma R.K. Role of oxidative stress in female reproduction. Reprod. Biol. Endocrinol., 2005, 3: 28 CrossRef
  20. Maia Mda S., Bicudo S.D., Sicherle C.C., Rodello L., Gallego I.C. Lipid peroxidation and generation of hydrogen peroxide infrozen-thawed ram semen cryopreserved in extenders with antioxidants. Anim. Reprod. Sci., 2010, 122(1-2): 118-123 CrossRef
  21. Silva S.V., Soares A.T., Batista A.M., Almeida F.C., Nunes J.F., Peixoto C.A., Guerra M.M. In vitro and in vivo evaluation of ram sperm frozen in tris egg-yolk and supplemented with superoxide dismutase and reduced glutathione. Reprod. Domest. Anim., 2011, 46(5): 874-881 CrossRef
  22. Ari U.Ç., Kulaksiz R., Yildiz S., Lehimcioglu N.C., Öztürkler Y. Effects of antioxidants in glutathione redox cycle on freezability of ram semen (Proc. 18th Annual Conference of the ESDAR, Helsinki, Finland, 2014). Abstracts. Reprod. Domest. Anim., 2014, 49(suppl. 3): 54 CrossRef Available https://www.researchgate.net/publication/278870707_Effect_of_antioxidants_
    in_glutathione_redox_cycle_on_freezability_of_ram_semen. Accessed March 31, 2017.
  23. Santiani  A., Evangelista S., Sepúlveda N., Risopatrón J., Villegas J., Sánchez R. Addition of superoxide dismutase mimics during cooling process prevents oxidative stress and improves semen quality parameters in frozen/thawed ram spermatozoa. Theriogenology, 2014, 82(6): 884-889 CrossRef
  24. Bucak M.N., Atessahin A., Varisli Ö., Yücel A., Tekin N., Akçay A. The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen Microscopic and oxidative stress parameters after freeze-thawing process. Theriogenology, 2007, 67(5): 1060-1067 CrossRef
  25. Panyaboriban S., Suwimonteerabutr J., Phutikanit N., Swangchan-Uthai T., Tharasanit T., Techakumphu M. Effect of various combinations of sugar supplementation in the extender on frozen-thawed ram semen quality and fertility. Thai. J. Vet. Med., 2015, 45(2): 229-237.
  26. Najafi A., Najafi M.H., Zanganeh Z., Sharafi M., Martinez-Pastor F., Adeldust H. Cryopreservation of ram semen in extenders containing soybean lecithin as cryoprotectant and hyaluronic acid as antioxidant. Reprod. Domest. Anim., 2014, 49(6): 934-940 CrossRef
  27. Emamverdi M., Zhandi M., Shahneh A.Z., Sharafi M., Akhlaghi A., Motlagh K.M., Dadkhah F., Davachi N.D. Flow cytometric and microscopic evaluation of post-thawed ram semen cryopreserved in chemically de?ned home-made or commercial extenders. Animal Production Science, 2015, 55(4): 551-558 CrossRef
  28. Mata-Campuzano M., Álvarez-Rodríguez M., Álvarez M., Tamayo-Canul J., Anel L., de Paz P., Martínez-Pastor F. Post-thawing quality and incubation resilience of cryopreserved ram spermatozoa are affected by antioxidant supplementation and choice of extender. Theriogenology, 2015, 83(4): 520-528 CrossRef
  29. Üstüner B., Alçay S., Nur Z., Sagirkaya H., Soylu M.K. Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility. Kafkas Univ. Vet. Fak. Derg., 2014, 20(3): 393-398 CrossRef
  30. Forouzanfar M., Sharafi M., Hosseini S.M., Ostadhosseini S., Hajian M., Hosseini L., Abedi P., Nili N., Rahmani H.R., Nasr-Esfahani M.H. In vitro comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of ram semen. Theriogenology, 2010, 73(4): 480-487 CrossRef
  31. Towhidi A., Zeinoaldini S., Ardebili R., Davachi N.D., Nasiri A.H. Combined w-3 fatty acids and a-tocopherol supplementation improved the ovine sperm cryosurvival. Iranian Journal of Biotechnology, 2013, 11(4): 238-243 CrossRef
  32. Talebiyan R., Amidi F., Samini M., Mirshokraei P., Dehkordi S.H. Effect of met-anandamide on prevention of hyperactivation, cryo-capacitation and acrosome reaction in ram semen cryopreservation. Kafkas Univ. Vet. Fak. Derg., 2015, 21(4): 545-551 CrossRef
  33. da Silva E.C.B., Cajueiro J.F. de P., Silva S.V., Guerra M.M.P. Ethylene  glycol  and  acetamide cryoprotectants on in vitro viability of thawed ram spermatozoa. Ciência Rural, 2012, 42(6): 1083-1088 CrossRef
  34. Chinoy N.J. Ascorbic acid levels in mammalian tissues and its metabolic significance. Comp. Biochem. Physiol. A. Comp. Physiol., 1972; 42(4): 945-952 CrossRef
  35. Buettner G.R. The pecking order of free radicals and antioxidants: lipid peroxidation, a-tocopherol, and ascorbate. Arch. Biochem. Biophys., 1993, 300(2): 535-543 CrossRef
  36. Fraga C.D., Motchnik P.A., Shinegana M.K., Helbock H.J., Jacob R.A., Ames B.N. Ascorbic acid protects against oxidative DNA damage in human sperm. PNAS USA, 1991, 88(24): 11003-11006 CrossRef
  37. Yildiz S., Daskin A. Koç spermasinin farkli antioksidan içeren sulandiricilarla kisa süreli saklanmasi. Kafkas Univ. Vet. Fak. Derg., 2004, 10(2): 155-159.
  38. Hamedani M.A., Tahmasbi A., Naserian A., Ahangari Y.J. Influence of added vitamin S on chilled and frozen-thawed ram sperm cryopreserved in tris extender. International Journal of Biology, Pharmacy and Allied Sciences, 2015, 4(9): 5848-5859
  39. Hamedani M.A., Tahmasbi A.M., Ahangari Y.J. Effects of vitamin supplementation on the quality of Ovine spermatozoa. Open Veterinary Journal, 2013, 3(2): 140-144.
  40. Cai J.G., Sun S.Q., Wang L.G., Gu H.J. The effect of adding vitamin B12 in sperm diluter on quality of bull’s straw frozen sperm. J. Liaoning Agricult. Coll., 2004, 6: 10-11.
  41. Appasamy M., Muttukrishna S., Pizzey A.R., Ozturk O., Groome N.P., Serhal P., Jauniaux E. Relationship between male reproductive hormones, sperm DNA damage and markers of oxidative stress in infertility. Reproductive BioMedicine Online, 2007, 14(2): 159-165 CrossRef
  42. Bellanti F., Matteo M., Rollo T., de Rosario F., Greco P., Vendemiale G. Sex hormones modulate circulating antioxidant enzymes: impact of estrogen therapy. Redox Biology, 2013, 19(1): 340-346 CrossRef
  43. Succu S., Berlinguer F., Pasciu V., Satta V., Leoni G.G., Naitana S. Melatonin protects ram spermatozoa from cryopreservation injuries in a dose-dependent manner. Journal of Pineal Research, 2011, 50(3): 310-318 CrossRef
  44. Das Graças C.P., Lim A. in P.G., Fidelis A.A.G., Cardoso J.R., Blume H., Mondadori R.G. Metil-formamida na criopreservação de sêmen ovino [Methyl-formamide in ram semen cryopreservation]. Ciênc. anim. bras., 2013, 14(4): 481-487 CrossRef
  45. Motamedi-Mojdehi R., Roostaei-Ali Mehr M., Rajabi-Toustani R. Effect of different levels of glycerol and cholesterol-loaded cyclodextrin on cryosurvival of ram spermatozoa. Reprod. Domest. Anim., 2014, 49(1): 65-70 CrossRef
  46. Gil J., Söderquist L., Rodriguez-Martinez H. Influence  of  centrifugation  and  different  extenders  on  post-thaw sperm  quality  of  ram semen. Theriogeneolgy, 2000, 54(1): 93-108 CrossRef
  47. Anel L., de Paz P., Álvarez M., Chamorro C.A., Boixo J.C., Manso A., González M., Kaabi M., Anel E. Field and in vitro assay of three methods for freezing ram semen. Theriogenology, 2003, 60(7): 1293-1308 CrossRef
  48. Öztürkler Y., Ak K., Ileri I.K. Koç spermasinin yogun gliserollü sulandiricilarda dondurulmasi. IstanbulUniv. Vet. Fak. Derg., 1999, 25(2): 399.
  49. Öztürkler Y., Ak K., Ileri I.K. The effect of season on post thaw spermatological properties in Kivircik rams. KafkasUniv. Vet. Fak. Derg., 1997, 3(1): 73-79.
  50. Frazão Sobrinho M., Castelo Branco M.A., Sousa A. Júnior, Nascimento I.M.R., Mota L.H.C.M., Carvalho Y.N.T., Ferreira S.B., Costa D.N.M., Moraes F.J. Júnior, Souza J.A.T. Características do sêmen de carneiros Dorper, Santa Inês e sem padrão racial definido, pré e pós-congelação, nos períodos chuvoso e seco [Characteristics of the semen of Dorper, Santa Ines and undefined breed sheep, pre- and post-freezing, in the rainy and dry period]. Arq. Bras. Med. Vet. Zootec., 2014, 66(4): 969-976 CrossRef

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