Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2017, V. 52, № 2, pp. 409-417
(how to cite this article)

doi: 10.15389/agrobiology.2017.2.409eng

UDC 636.085.19:636.086.2/.3:632.4(470)

Acknowledgements:
Supported in part by Russian Science Foundation (project № 14-16-00114)

 

MYCOTOXINS IN THE LEGUMES OF NATURAL FODDER
OF THE EUROPEAN RUSSIA

A.A. Burkin1, G.P. Kononenko1, O.P. Gavrilovav2, T.Yu. Gagkaeva2

1All-Russian Research Institute of Sanitary, Hygiene and Ecology, Federal Agency of Scientific Organizations, 5, Zvenigorodskoe sh., Moscow, 123022 Russia,
e-mail kononenkogp@mail.ru (corresponding author);
2All-Russian Research Institute of Plant Protection, Federal Agency of Scientific Organizations,3, sh. Podbel’skogo, St. Petersburg, 196608 Russia,
e-mail t.gagkaeva@yahoo.com (corresponding author)

The authors declare no conflict of interests

ORCID:
KononenkoG.P. orcid.org/0000-0002-9144-615X

Received August 26, 2016

 

Evaluation of the negative effects of mycotoxins on ruminants and horses is a complex scientific problem and has important economic significance. Mycotoxicoses of animals caused by feeding leguminous grasses have been known for a long time, but their causes remain largely unclear. The purpose of this work was a comparative study of mycotoxin contamination of legumes of the genera Lathyrus, Trifolium, Vicia, Melilotus, Medicago, Galega and Lupinus from the natural fodder lands of the European part of Russia. The collection of ground parts of plants was carried out in May-September 2015 in the Moscow, Tver, Leningrad, Pskov, Novgorod, Smolensk, Astrakhan regions, Perm Krai, and the Republic of Karelia. The mycotoxins determined by enzyme immunoassay were T-2 toxin (T-2), diacetoxycirpenol (DAS), deoxynivalenol (DON), zearalenone (ZEN), fumonisins (FUM), alternariol (AOL), roridin A (POA), aflatoxin B1 (AB1), sterigmatocystin (STE), cyclopiazonic acid (CPA), emodin (EMO), ochratoxin A (OA), citrinin (CIT), mycophenolic acid (MPA), PR toxin (PR) and ergot alkaloids (EA). In grasses of the genus Lathyrus (peavines), all the analyzed mycotoxins (with the exception of FUM and ROA) were found in more than 80 % of the samples; for clovers (Trifolium), the same frequency was found in T-2, OA, MPA, EA, AOL, CPA and EMO, and in species of the genus Vicia (vetchs), sweetclovers (Melilotus) and alfalfa (Medicago) there were only EA, AOL, CPA and EMO. A common feature for the genera Lathyrus, Trifolium and Vicia plants were high, > 1000 μg/kg, accumulation of DAS, AOL and CPA, and for peavines and clovers, PR also. Among the peculiarities of meadow clover (T. pratense L.), we found the ultra-high content of EMO, up to 30 000 μg/kg and more. White clover (T. repens L.) was characterized by moderate contamination as compared to other species of the genus. Representatives of Vicia,  bush vetch (V. sepium L.) and cow vetch (V. cracca L.), showed similarities in the frequency of the majority of mycotoxins, but in cow vetch with a smaller occurrence in comparison to bush vetch, especially visible for FUM, the upper limits of the contents of EA, AOL, POA, STE, MPA were higher. Meadow sweetclovers and alfalfa were found to be contaminated less than other grasses, while sweetclovers were close to Vicia in terms of detection frequency and amounts of OA and PR, and alfalfa was low in MPA and relatively high in EMO accumulation. Among other crops less common in meadows, Lupinus polyphyllus Lindl. had the highest mycotoxicological load — of 16 metabolites analyzed, all but FUM and ROA were more than 80 % in frequency. At Caucasian goat’s rue (Galega orientalis Lam.) and narrow-leaved vetch (Vicia sativa L.) mycotoxins were detected less often and in smaller quantities. In this study previously published data on mycotoxicological analysis for meadow clover and white clover (A.A. Burkin and G.P. Kononenko, 2015) have been confirmed, but for other legumes the findings are presented for the first time.

Keywords: meadow grasses, legumes, Lathyrus, Trifolium, Vicia, Melilotus, Medicago, Galega, Lupinus, mycotoxins, T-2 toxin (T-2), diacetoxycirpenol (DAS), deoxynivalenol (DON), zearalenone (ZEN), fumonisins (FUM), alternariol (AOL), roridin A (POA), aflatoxin B1 (AB1), sterigmatocystin (STE), cyclopiazonic acid (CPA), emodin (EMO), ochratoxin A (OA), citrinin (CIT), mycophenolic acid (MPA), PR toxin (PR), ergot alkaloids (EA).

 

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REFERENCES

  1. Fink-Gremmels J. V knige: Mikotoksiny i mikotoksikozy /Pod redaktsiei D. Diaza [Mycotoxins and mycotoxicoses. D. Diaz (ed.)]. Moscow, 2006: 157-178 (in Russ.).
  2. Kuldau G.A., Mansfield M.A., Jones A.D., Archibald D.D., De Wolf E.D. The less understood: mycotoxins and fungi in forages in silages. Proc. The World Mycotoxin Forum® — the fifth conference. Noordwijk aan Zee, the Nethwerlands, 2008: 34.
  3. Nedelnik J., Strejckova M., Cholastova T., Both Z., Palicova J., Hortova B., Hopkins A., Collins R.P., Fraser M.D., King V.R., Lloyd D.C., Moorby J.M. Robson P.R. Feeding, mycological and toxicological quality of haylage. Grassland Science in Europe, 2014, 19: 606-609.
  4. Gallo A., Giuberti G., Frisvad J.C., Bertuzzi T., Nielsen K.F. Review on mycotoxin issues in ruminants: occurrence in forages, effects of mycotoxin ingestion on health status and animal performance and practical strategies to counteract their negative effects. Toxins, 2015, 7: 3057-3111CrossRef
  5. Kononenko G.P., Burkin A.A. About fusariotoxins contamination of cereals used for fodder. Agricultural Biology, 2009, 4: 81-88 (in Russ.).
  6. Gavrilova O.P., Gagkaeva T.Yu., Burkin A.A., Kononenko G.P. Mycological infection by fusarium strains and mycotoxins contamination of oats and barley grain in the north of Nonchernozem’e.Agricultural Biology, 2009, 6: 89-93 (in Russ.).
  7. Kononenko G.P., Burkin A.A. Fusariotoxins content in maize and rice grain harvested in the main regions of cultivation in the Russian Federation. Agricultural Biology, 2008, 5: 88-91 (in Russ.).
  8. Burkin A.A., Kononenko G.P., Soboleva N.A. Doklady Rossiiskoi akademii sel'skokhozyaistvennykh nauk, 2005, 2: 47-49 (in Russ.).
  9. Kononenko G.P., Burkin A.A. Peculiarities of feed contamination with citrinin and ochratoxin A. Agricultural Sciences, 2013, 4(1): 34-38 CrossRef
  10. Gagkaeva T.Yu., Gavrilova O.P., Kononenko G.P., Burkin A.A. Sovremennaya mikologiya v Rossii, 2015, 5(5): 230-232 CrossRef (in Russ.)
  11. Gagkaeva T.Yu., Gavrilova O.P., Burkin A.A., Kononenko G.P. Fusarium fungi and mycotoxins on cultivated forage grasses. Proc. 13th European Fusarium Seminar dedicated to the Memory of Wally Marasas «Fusarium — Pathogenicity, Mycotoxins, Taxonomy, Genomics, Biosynthesis, Metabolomics, Resistance, Disease control». Martina Franca, Italy, 2015: 149.
  12. Kononenko G.P., Burkin A.A., Gavrilova O.P., Gagkaeva T.Yu. Fungal species and multiple mycotoxin contamination of cultivated grasses and legumes crops. Agricultural and Food Science, 2015, 24: 323-330.
  13. Kononenko G.P., Burkin A.A. Mycotoxin contaminations in commercially used hay. Agricultural Biology, 2014, 4: 120-126 CrossRef (in Russ.).
  14. Kononenko G.P., Burkin A.A. Mycotoxin contaminations in commercially used haylage and silage. Agricultural Biology, 2014, 6: 116-122 CrossRef (in Engl.).
  15. Laptev G. Yu., Novikova N.I., Il'ina L.A., Yyldyrym E.A., Soldatova V.V., Nikonov I.N., Filippova V.A., Brazhnik E.A., Sokolova O.N. Dynamics of mycotoxin accumulation in silage during storage. Agricultural Biology, 2014, 6: 123-130 CrossRef (in Engl.).
  16. Burkin A.A., Kononenko G.P., Gavrilova O.P., Gagkaeva T.Yu. About zearalenone levels in grass fodders and toxine producing activity of Fusarium fungi. Agricultural Biology, 2015, 50(2): 255-262 CrossRef (in Engl.).
  17. Burkin A.A., Kononenko G.P. Mycotoxin contamination of meadow grasses in European Russia. Agricultural Biology, 2015, 50(4): 503-512 CrossRef (in Engl.).
  18. Gubanov I.A., Kiseleva K.V., Novikov V.S., Tikhomirov V.N. Illyustrirovannyi opredelitel' rastenii Srednei Rossii. Tom 2 [Illustrated identification keys of plants in Central Russia. V. 2]. Moscow, 2003 (in Russ.).
  19. Skvortsov V.E. Illyustrirovannoe rukovodstvo dlya botanicheskikh praktik i ekskursii v Srednei Rossii [Illustrated guide for botanical practics and excursions in Central Russia]. Moscow, 2004 (in Russ.).
  20. Burkin A.A., Kononenko G.P. Prikladnaya biokhimiya i mikrobiologiya, 2013, 49(5): 522-530 CrossRef (in Russ.).
  21. Korneichuk N.S., Tkachenko N.V. V sbornike: Problemy mikologii i fitopatologii v XXI veke [In: Chellenges in mycology and phytopathology in XXI century]. St. Petersburg, 2013: 161-163 (in Russ.).
  22. Burkin A.A., Kononenko G.P. Izvestiya RAN. Seriya biologicheskaya, 2014, 3: 228-235 CrossRef (in Russ.).
  23. Kusari S., Spiteller M. Metabolomics of endophytic fungi producing associated plant secondary metabolites: progress, challenges and opportunities. In: Metabolomics. U. Roessner (ed.). IN TECH d.o.o, Rijeka, Croatia, 2012.
  24. Cook D., Gardner D.R., Pfister J.A., Grum D. Biosynthesis of natural products in plants by fungal endophytes with an emphasis on swainsonine. In: Phytochemicals — biosynthesis, function and application, recent advances in phytochemistry. V. 44. R. Jetter (ed.). Springer International Publishing Switzerland, 2014: 23-32 CrossRef
  25. Cheeke P.R. Endogenous toxins and mycotoxins in forage grasses and the effects on livestock. J. Anim. Sci., 1995, 73(3): 909-918.
  26. Kusari S., Zühlke S., Kosuth J., Cellarova E., Spiteller M. Light-inde-
    pendent metabolomics of endophytic Thielavia subthermophila provides insight into microbial hypericin biosynthesis. J. Nat. Prod., 2009, 72: 1825-1835.
  27. Ravindra K.N., Thoyajaksha, Narayanappa M., Sharanappa P. Study of endophytic fungal community from bark of Ventilago madrasapatna Gaertn. International Journal of Pharma and Bio Sciences, 2013, 4(1): 309-316.
  28. Skalozub O.M. Kormoproizvodstvo, 2012, 12: 7-8 (in Russ.).
  29. Reshetnikova E.D. Kormoproizvodstvo, 2012, 12: 9-12 (in Russ.).

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